Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species

Detalhes bibliográficos
Autor(a) principal: Nogueira, Paula Monalisa
Data de Publicação: 2020
Outros Autores: Menezes Neto, Armando de, Borges, Valéria de Matos, Descoteaux, Albert, Torrecilhas, Ana Claudia, Xander, Patrícia, Revach, Or-Yam, Regev-Rudzki, Neta, Soares, Rodrigo Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43527
Resumo: FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ- 01100-14 and PPM-X 00102-16) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico) (302972/2019- 6, 405336/2017-9 and 431857/2018-0). AM-N was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) (AUXPE 1526/2011). AT and PX was supported by FAPESP (Fundação de Amparo do Estado de São Paulo (2016-01917-3 and 2016/172454) and CNPq (302104/2019- 4). AD holds the Canada Research Chair on the Biology of Intracellular Parasitism.
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spelling Nogueira, Paula MonalisaMenezes Neto, Armando deBorges, Valéria de MatosDescoteaux, AlbertTorrecilhas, Ana ClaudiaXander, PatríciaRevach, Or-YamRegev-Rudzki, NetaSoares, Rodrigo Pedro2020-09-21T17:52:31Z2020-09-21T17:52:31Z2020NOGUEIRA, Paula Monalisa et al. Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species. Frontiers in Cellular and Infection Microbiology, p. 1-9, Jul. 2020.2235-2988https://www.arca.fiocruz.br/handle/icict/4352710.3389/fcimb.2020.00380FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ- 01100-14 and PPM-X 00102-16) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico) (302972/2019- 6, 405336/2017-9 and 431857/2018-0). AM-N was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) (AUXPE 1526/2011). AT and PX was supported by FAPESP (Fundação de Amparo do Estado de São Paulo (2016-01917-3 and 2016/172454) and CNPq (302104/2019- 4). AD holds the Canada Research Chair on the Biology of Intracellular Parasitism.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Université du Québec. Centre Armand-Frappier Santé Biotechnologie. Laval, QC, Canada.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Diadema, SP, Brasil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Diadema, SP, Brasil.Weizmann Institute of Science. Department of Biomolecular Sciences Rehovot. Israel.Weizmann Institute of Science. Department of Biomolecular Sciences Rehovot. Israel.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Leishmania infection causes considerable human morbidity and may develop into a deadly visceral form in endemic regions. The parasite infects macrophages where they can replicate intracellularly. Furthermore, they modulate host immune responses by using virulence factors (lipophosphoglycan, glycoprotein-63, and others) that promote survival inside the cells. Extracellular vesicles (EVs) released by parasites are important for cell-cell communication in the proinflammatory milieu modulating the establishment of infection. However, information on the ability of EVs from different Leishmania species to modulate inflammatory responses is scarce, especially from those species causing different clinical manifestations (visceral vs. cutaneous). The purpose of this study was to compare macrophage activation using EVs from three Leishmania species from New World including L. infantum, L. braziliensis, and L. amazonensis. EVs were released from promastigote forms, purified by ultracentrifugation and quantitated by Nanoparticle Tracking Analysis (NTA) prior to murine macrophage exposure. NTA analysis did not show any differences in the EV sizes among the strains. EVs from L. braziliensis and L. infantum failed to induce a pro-inflammatory response. EVs from both L. infantum WT and LPG-deficient mutant (LPG-KO) did not show any differences in their interaction with macrophages, suggesting that LPG solely was not determinant for activation. On the other hand, EVs from L. amazonensis were immunomodulatory inducing NO, TNF-a, IL-6, and IL-10 via TLR4 and TLR2. To determine whether such activation was related to NF-kB p65 translocation, THP-1 macrophage cells were exposed to EVs. In the same way, only EVs from L. amazonensis exhibited a highly percentage of cells positive for NF-kB. Our results suggest an important role of EVs in determining the pattern of immune response depending on the parasite species. For L. infantum, LPG was not determinant for the activation.engFrontiers MediaVesículas extracelularesLeishmaniaInterações hospedeiro-parasitaImunidade inataLipofosfoglicanoExtracellular vesiclesLeishmaniaHost-parasite interactionInnate immunityLipophosphoglycanImmunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43527/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNogueira, Paula Monalisa Immomodulatory....pdfNogueira, Paula Monalisa 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dc.title.pt_BR.fl_str_mv Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
title Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
spellingShingle Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
Nogueira, Paula Monalisa
Vesículas extracelulares
Leishmania
Interações hospedeiro-parasita
Imunidade inata
Lipofosfoglicano
Extracellular vesicles
Leishmania
Host-parasite interaction
Innate immunity
Lipophosphoglycan
title_short Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
title_full Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
title_fullStr Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
title_full_unstemmed Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
title_sort Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species
author Nogueira, Paula Monalisa
author_facet Nogueira, Paula Monalisa
Menezes Neto, Armando de
Borges, Valéria de Matos
Descoteaux, Albert
Torrecilhas, Ana Claudia
Xander, Patrícia
Revach, Or-Yam
Regev-Rudzki, Neta
Soares, Rodrigo Pedro
author_role author
author2 Menezes Neto, Armando de
Borges, Valéria de Matos
Descoteaux, Albert
Torrecilhas, Ana Claudia
Xander, Patrícia
Revach, Or-Yam
Regev-Rudzki, Neta
Soares, Rodrigo Pedro
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Paula Monalisa
Menezes Neto, Armando de
Borges, Valéria de Matos
Descoteaux, Albert
Torrecilhas, Ana Claudia
Xander, Patrícia
Revach, Or-Yam
Regev-Rudzki, Neta
Soares, Rodrigo Pedro
dc.subject.other.pt_BR.fl_str_mv Vesículas extracelulares
Leishmania
Interações hospedeiro-parasita
Imunidade inata
Lipofosfoglicano
topic Vesículas extracelulares
Leishmania
Interações hospedeiro-parasita
Imunidade inata
Lipofosfoglicano
Extracellular vesicles
Leishmania
Host-parasite interaction
Innate immunity
Lipophosphoglycan
dc.subject.en.pt_BR.fl_str_mv Extracellular vesicles
Leishmania
Host-parasite interaction
Innate immunity
Lipophosphoglycan
description FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ- 01100-14 and PPM-X 00102-16) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico) (302972/2019- 6, 405336/2017-9 and 431857/2018-0). AM-N was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) (AUXPE 1526/2011). AT and PX was supported by FAPESP (Fundação de Amparo do Estado de São Paulo (2016-01917-3 and 2016/172454) and CNPq (302104/2019- 4). AD holds the Canada Research Chair on the Biology of Intracellular Parasitism.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-21T17:52:31Z
dc.date.available.fl_str_mv 2020-09-21T17:52:31Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOGUEIRA, Paula Monalisa et al. Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species. Frontiers in Cellular and Infection Microbiology, p. 1-9, Jul. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43527
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.pt_BR.fl_str_mv 10.3389/fcimb.2020.00380
identifier_str_mv NOGUEIRA, Paula Monalisa et al. Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-kB Translocation by Dermotropic and Viscerotropic Species. Frontiers in Cellular and Infection Microbiology, p. 1-9, Jul. 2020.
2235-2988
10.3389/fcimb.2020.00380
url https://www.arca.fiocruz.br/handle/icict/43527
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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