Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy

Detalhes bibliográficos
Autor(a) principal: Santos, Adalberto Rezende
Data de Publicação: 2001
Outros Autores: Balassiano, Vivian, Oliveira, Maria Leide W., Pereira, Marcia Aparecida da Silva, Santos, Patricia Barros, Degrave, Wim M., Suffys, Philip Noel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02762001000800017
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36818
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Setor de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Adalberto RezendeBalassiano, VivianOliveira, Maria Leide W.Pereira, Marcia Aparecida da SilvaSantos, Patricia BarrosDegrave, Wim M.Suffys, Philip Noel2019-11-02T21:07:32Z2019-11-02T21:07:32Z2001SANTOS, Adalberto Rezende et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1129-1133, Nov. 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/3681810.1590/S0074-027620010008000171678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Mycobacterium lepraeReação em cadeia de polimeraseDiagnósticoTerapiaPós-tratamentoHanseníaseMycobacterium lepraePCRDiagnosisTherapyPost-treatmentPaucibacillary leprosyDetection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Setor de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Diagnóstico de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Thirty eight patients with indeterminate leprosy (HI), at least 4 to 6 years after discharge from multibacillary (MB) or paucibacillary (PB) schemes of anti leprosy multidrug therapy (MDT), were submitted to traditional diagnostic procedures for leprosy and to polymerase chain reaction (PCR) analysis of different clinical samples for detection of Mycobacterium leprae DNA. No significant difference was observed for any of the parameters analyzed between PB or MB schemes of treatment and no indications were found for more efficient outcome of HI using the MB scheme. Remarkably, 18 (54.5%) of the individuals were PCR positive in at least one of the samples: positivity of PCR was highest in blood samples and four individuals were PCR positive in blood and some other sample. Upon comparison of PCR results with clinical and histopathological parameters, no correlation was found between PCR-positivity and eventual relapse. This is the first report on detection of M. leprae DNA in PB patients, more than half a decade after completion of MDT, suggesting that live bacilli are present and circulating much longer than expected, although reinfection of the individuals can not be excluded. Overall, we feel that because of the high sensitivity of the assay, extreme care should be taken about association of PCR results, efficacy of treatment and disease status.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36818/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPatriciaSantos_PhilipSuffys_etal_IOC_2001.pdfPatriciaSantos_PhilipSuffys_etal_IOC_2001.pdfapplication/pdf52788https://www.arca.fiocruz.br/bitstream/icict/36818/2/PatriciaSantos_PhilipSuffys_etal_IOC_2001.pdf1a3ee8fb3ef6043f8eeb9a02ca6b8ecfMD52TEXTPatriciaSantos_PhilipSuffys_etal_IOC_2001.pdf.txtPatriciaSantos_PhilipSuffys_etal_IOC_2001.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
title Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
spellingShingle Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
Santos, Adalberto Rezende
Mycobacterium leprae
Reação em cadeia de polimerase
Diagnóstico
Terapia
Pós-tratamento
Hanseníase
Mycobacterium leprae
PCR
Diagnosis
Therapy
Post-treatment
Paucibacillary leprosy
title_short Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
title_full Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
title_fullStr Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
title_full_unstemmed Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
title_sort Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy
author Santos, Adalberto Rezende
author_facet Santos, Adalberto Rezende
Balassiano, Vivian
Oliveira, Maria Leide W.
Pereira, Marcia Aparecida da Silva
Santos, Patricia Barros
Degrave, Wim M.
Suffys, Philip Noel
author_role author
author2 Balassiano, Vivian
Oliveira, Maria Leide W.
Pereira, Marcia Aparecida da Silva
Santos, Patricia Barros
Degrave, Wim M.
Suffys, Philip Noel
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Adalberto Rezende
Balassiano, Vivian
Oliveira, Maria Leide W.
Pereira, Marcia Aparecida da Silva
Santos, Patricia Barros
Degrave, Wim M.
Suffys, Philip Noel
dc.subject.other.pt_BR.fl_str_mv Mycobacterium leprae
Reação em cadeia de polimerase
Diagnóstico
Terapia
Pós-tratamento
Hanseníase
topic Mycobacterium leprae
Reação em cadeia de polimerase
Diagnóstico
Terapia
Pós-tratamento
Hanseníase
Mycobacterium leprae
PCR
Diagnosis
Therapy
Post-treatment
Paucibacillary leprosy
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
PCR
Diagnosis
Therapy
Post-treatment
Paucibacillary leprosy
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Setor de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2019-11-02T21:07:32Z
dc.date.available.fl_str_mv 2019-11-02T21:07:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Adalberto Rezende et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1129-1133, Nov. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36818
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762001000800017
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SANTOS, Adalberto Rezende et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1129-1133, Nov. 2001.
0074-0276
10.1590/S0074-02762001000800017
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36818
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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