Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50158 |
Resumo: | Bahia Research Support Foundation (Fapesb), grant number 9092/2015. The authors acknowledge CNPq, Capes and Fapesb for financial support via scholarships. |
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Luz, Yasmin da SilvaPaixão, Amanda R.Bernardes, Carla Polyana O. S.Silva, Thaílla Souza daTavares, Natalia MachadoBrodskyn, Claudia IdaVeras, Patricia Sampaio TavaresMenezes, Juliana Pereira Bezerra de2021-12-03T17:08:13Z2021-12-03T17:08:13Z2021LUZ, Yasmin da Silva et al. Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction. Journal of Visualized Experiments, 2021.1940-087Xhttps://www.arca.fiocruz.br/handle/icict/5015810.3791/62461 (2021).Bahia Research Support Foundation (Fapesb), grant number 9092/2015. The authors acknowledge CNPq, Capes and Fapesb for financial support via scholarships.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil.Leishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-resolving localized cutaneous lesions to a highly fatal visceral form of the disease. An estimated 12 million people worldwide are currently infected, and another 350 million face risk of infection. It is known that host cells infected by Leishmania parasites, such as macrophages or dendritic cells, can migrate to different host tissues, yet how migration contributes to parasite dissemination and homing remains poorly understood. Therefore, assessing these parasites' ability to modulate host cell response, adhesion, and migration will shed light on mechanisms involved in disease dissemination and visceralization. Cellular migration is a complex process in which cells undergo polarization and protrusion, allowing them to migrate. This process, regulated by actin and tubulinbased microtubule dynamics, involves different factors, including the modulation of cellular adhesion to the substrate. Cellular adhesion and migration processes have been investigated using several models. Here, we describe a method to characterize the migratory aspects of host cells during Leishmania infection. This detailed protocol presents the differentiation and infection of dendritic cells, the analysis of host cell motility and migration, and the formation of adhesion complexes and actin dynamics. This in vitro protocol aims to further elucidate mechanisms involved in Leishmania dissemination within vertebrate host tissues and can also be modified and applied to other cell migration studies.engMyJove CorporationLeishmaniaCélulas DendríticasMacrófagosAdesão CelularLeishmaniasisDendritic cellsMacrophagesCell adhesionCell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50158/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALLuz, Yasmin da Silva cell-migration-cell-adhesion-assays... (1).pdfLuz, Yasmin da Silva cell-migration-cell-adhesion-assays... (1).pdfapplication/pdf687941https://www.arca.fiocruz.br/bitstream/icict/50158/2/Luz%2c%20Yasmin%20da%20Silva%20cell-migration-cell-adhesion-assays...%20%281%29.pdf928a4cc49c095b1da8861b6cc9cf1fcbMD52icict/501582023-03-15 14:34:32.674oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| title |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| spellingShingle |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction Luz, Yasmin da Silva Leishmania Células Dendríticas Macrófagos Adesão Celular Leishmaniasis Dendritic cells Macrophages Cell adhesion |
| title_short |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| title_full |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| title_fullStr |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| title_full_unstemmed |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| title_sort |
Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction |
| author |
Luz, Yasmin da Silva |
| author_facet |
Luz, Yasmin da Silva Paixão, Amanda R. Bernardes, Carla Polyana O. S. Silva, Thaílla Souza da Tavares, Natalia Machado Brodskyn, Claudia Ida Veras, Patricia Sampaio Tavares Menezes, Juliana Pereira Bezerra de |
| author_role |
author |
| author2 |
Paixão, Amanda R. Bernardes, Carla Polyana O. S. Silva, Thaílla Souza da Tavares, Natalia Machado Brodskyn, Claudia Ida Veras, Patricia Sampaio Tavares Menezes, Juliana Pereira Bezerra de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Luz, Yasmin da Silva Paixão, Amanda R. Bernardes, Carla Polyana O. S. Silva, Thaílla Souza da Tavares, Natalia Machado Brodskyn, Claudia Ida Veras, Patricia Sampaio Tavares Menezes, Juliana Pereira Bezerra de |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Células Dendríticas Macrófagos Adesão Celular |
| topic |
Leishmania Células Dendríticas Macrófagos Adesão Celular Leishmaniasis Dendritic cells Macrophages Cell adhesion |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Dendritic cells Macrophages Cell adhesion |
| description |
Bahia Research Support Foundation (Fapesb), grant number 9092/2015. The authors acknowledge CNPq, Capes and Fapesb for financial support via scholarships. |
| publishDate |
2021 |
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2021-12-03T17:08:13Z |
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2021-12-03T17:08:13Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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LUZ, Yasmin da Silva et al. Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction. Journal of Visualized Experiments, 2021. |
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https://www.arca.fiocruz.br/handle/icict/50158 |
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1940-087X |
| dc.identifier.eissn.none.fl_str_mv |
10.3791/62461 (2021). |
| identifier_str_mv |
LUZ, Yasmin da Silva et al. Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction. Journal of Visualized Experiments, 2021. 1940-087X 10.3791/62461 (2021). |
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https://www.arca.fiocruz.br/handle/icict/50158 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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MyJove Corporation |
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MyJove Corporation |
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