Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)

Detalhes bibliográficos
Autor(a) principal: Carvalho, Edirsana Maria Ribeiro
Data de Publicação: 2022
Outros Autores: Menezes, Francisca Gleire Rodrigues de, Santos, Daniel Rodrigues dos, Souza, Oscarina Viana de, Hofer, Ernesto, Vieira, Regine Helena Silva dos Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.35265/2236-6717-222-12082
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53557
Resumo: O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacEΔ1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão.
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spelling Carvalho, Edirsana Maria RibeiroMenezes, Francisca Gleire Rodrigues deSantos, Daniel Rodrigues dosSouza, Oscarina Viana deHofer, ErnestoVieira, Regine Helena Silva dos Fernandes2022-06-28T17:15:12Z2022-06-28T17:15:12Z2022CARVALHO, Edirsana Maria Ribeiro et al. Integrons adn gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (Litopenaeus Vannamei). Semana Acadêmica, Fortaleza, v. 10, Ed.222, p. 1 - 16, 2022.2236-6717https://www.arca.fiocruz.br/handle/icict/5355710.35265/2236-6717-222-12082O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacEΔ1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão.The purpose of the present study was to identify integrons and gene cassettes in vibrios isolated from the hemolymph of shrimp (Litopenaeus vannamei) farmed in Ceará (Northeastern Brazil). Twenty-five vibrio strains were evaluated on PCR for class 1 integrons in chromosomal and plasmidial DNA, using the primers In, Int1, qacEΔ1, sul1 and blaP1. Eight (32%) isolates yielded integrase (intI1) in chromosomal DNA belonging to class 1 integrons, with the gene cassette blaP1 detected in strains of V. natriengens (V10), V. coralliilyticus (V11) and V. parahaemolyticus (V25). The percentage was 100% in plasmids, with the detection of the gene cassettes blaP1 and ereA2 in V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) and V. mimicus (V22). The detection of antimicrobial resistance gene cassettes in the isolated strains indicates the possibility of dissemination of these genetic elements to autochthonous bacteria in shrimp culture environment.Centro Universitário Unifanor. Professor. Fortaleza, CE, Brasil.Centro Universitário Unifanor. Departamento de Engenharia de Pescas. Professor. Fortaleza, CE, Brasil.Cientista Ambiental.Universidade Federal do Ceará. Instituto de Ciências do Mar. Professor. Labomar. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Pesquisador. Rio de Janeiro, RJ, Brasil.Universidade Federal do Ceará. Instituto de Ciências do Mar. Professor. 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dc.title.pt_BR.fl_str_mv Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
title Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
spellingShingle Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
Carvalho, Edirsana Maria Ribeiro
Aquacultura
Antibióticos
Bactéria
Integron
Aquaculture
Antibiotic
Bacteria
Integron
title_short Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
title_full Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
title_fullStr Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
title_full_unstemmed Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
title_sort Integrons and Gene Cassettes Encoding Antimicrobial resistace in Vibrios isolated from Shrimp Hemolymph (LitoPenaeus Vannamei)
author Carvalho, Edirsana Maria Ribeiro
author_facet Carvalho, Edirsana Maria Ribeiro
Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena Silva dos Fernandes
author_role author
author2 Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena Silva dos Fernandes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Edirsana Maria Ribeiro
Menezes, Francisca Gleire Rodrigues de
Santos, Daniel Rodrigues dos
Souza, Oscarina Viana de
Hofer, Ernesto
Vieira, Regine Helena Silva dos Fernandes
dc.subject.other.pt_BR.fl_str_mv Aquacultura
Antibióticos
Bactéria
Integron
topic Aquacultura
Antibióticos
Bactéria
Integron
Aquaculture
Antibiotic
Bacteria
Integron
dc.subject.en.pt_BR.fl_str_mv Aquaculture
Antibiotic
Bacteria
Integron
description O objetivo do presente estudo foi identificar integrons e cassetes gênicos em vibrios isolados da hemolinfa de camarão (Litopenaeus vannamei) cultivado no Ceará (Nordeste do Brasil). Vinte e cinco linhagens de vibrio foram avaliadas em PCR para integrons classe 1 em DNA cromossômico e plasmidial, utilizando os primers In, Int1, qacEΔ1, sul1 e blaP1. Oito (32%) isolados produziram integrase (intI1) em DNA cromossômico pertencente a integrons classe 1, com o gene cassete blaP1 detectado em cepas de V. natriengens (V10), V. coralliilyticus (V11) e V. parahaemolyticus (V25). A porcentagem foi de 100% em plasmídeos, com a detecção dos cassetes gênicos blaP1 e ereA2 em V. vulnificus B1 (V4), V. coralliilyticus (V7), V. alginolyticus (V8), V. harveyi (V9), V. alginolyticus (V16), V. harveyi (V18), V. parahaemolyticus (V19), V. parahaemolyticus (V21) e V. mimicus (V22). A detecção de cassetes de genes de resistência antimicrobiana nas cepas isoladas indica a possibilidade de disseminação desses elementos genéticos para bactérias autóctones em ambiente de cultivo de camarão.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-28T17:15:12Z
dc.date.available.fl_str_mv 2022-06-28T17:15:12Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Edirsana Maria Ribeiro et al. Integrons adn gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (Litopenaeus Vannamei). Semana Acadêmica, Fortaleza, v. 10, Ed.222, p. 1 - 16, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53557
dc.identifier.issn.pt_BR.fl_str_mv 2236-6717
dc.identifier.doi.none.fl_str_mv 10.35265/2236-6717-222-12082
identifier_str_mv CARVALHO, Edirsana Maria Ribeiro et al. Integrons adn gene cassettes encoding antimicrobial resistance in vibrios isolated from shrimp hemolymph (Litopenaeus Vannamei). Semana Acadêmica, Fortaleza, v. 10, Ed.222, p. 1 - 16, 2022.
2236-6717
10.35265/2236-6717-222-12082
url https://www.arca.fiocruz.br/handle/icict/53557
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Unieducar Inteligência Educacional Ltda
publisher.none.fl_str_mv Unieducar Inteligência Educacional Ltda
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https://www.arca.fiocruz.br/bitstream/icict/53557/2/Ernesto_Hoffer_etal_IOC_2022.pdf
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