Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine

Detalhes bibliográficos
Autor(a) principal: Medina, Jorge Mansur
Data de Publicação: 2015
Outros Autores: Rodrigues, Juliany Cola Fernandes, Moreira, Otacilio C., Atella, Geórgia, Souza, Wanderley de, Barrabin, Hector
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12240
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
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spelling Medina, Jorge MansurRodrigues, Juliany Cola FernandesMoreira, Otacilio C.Atella, GeórgiaSouza, Wanderley deBarrabin, Hector2015-11-16T14:49:59Z2015-11-16T14:49:59Z2015MEDINA, Jorge Mansur; et al. Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine. Mem Inst Oswaldo Cruz, v.110, n.1, p. 48-55, Feb. 2015.0074-0276https://www.arca.fiocruz.br/handle/icict/1224010.1590/0074-027601400971678-8060engFundação Oswaldo CruzMechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Núcleo Multidisciplinar de Pesquisa em Biologia. Duque de Caxias, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Phytomonas serpens are flagellates in the family Trypanosomatidae that parasitise the tomato plant (Solanum lycopersicum L.), which results in fruits with low commercial value. The tomato glycoalkaloid tomatine and its aglycone tomatidine inhibit the growth of P. serpens in axenic cultures. Tomatine, like many other saponins, induces permeabilisation of the cell membrane and a loss of cell content, including the cytosolic enzyme pyruvate kinase. In contrast, tomatidine does not cause permeabilisation of membranes, but instead provokes morphological changes, including vacuolisation. Phytomonas treated with tomatidine show an increased accumulation of labelled neutral lipids (BODYPY-palmitic), a notable decrease in the amount of C24-alkylated sterols and an increase in zymosterol content. These results are consistent with the inhibition of 24-sterol methyltransferase (SMT), which is an important enzyme that is responsible for the methylation of sterols at the 24 position. We propose that the main target of tomatidine is the sterols biosynthetic pathway, specifically, inhibition of the 24-SMT. Altogether, the results obtained in the present paper suggest a more general effect of alkaloids in trypanosomatids, which opens potential therapeutic possibilities for the treatment of the diseases caused by these pathogens.PhytomonasTomatineTomatidineLipidsTrypanosomatidsTomatinaLipídeosTrypanosomatinainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12240/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALotacilio_moreira_etal_IOC_2015.pdfotacilio_moreira_etal_IOC_2015.pdfapplication/pdf934644https://www.arca.fiocruz.br/bitstream/icict/12240/2/otacilio_moreira_etal_IOC_2015.pdfb5666f60e8535e3c775980b40a7d7759MD52TEXTotacilio_moreira_etal_IOC_2015.pdf.txtotacilio_moreira_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
title Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
spellingShingle Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
Medina, Jorge Mansur
Phytomonas
Tomatine
Tomatidine
Lipids
Trypanosomatids
Tomatina
Lipídeos
Trypanosomatina
title_short Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
title_full Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
title_fullStr Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
title_full_unstemmed Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
title_sort Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine
author Medina, Jorge Mansur
author_facet Medina, Jorge Mansur
Rodrigues, Juliany Cola Fernandes
Moreira, Otacilio C.
Atella, Geórgia
Souza, Wanderley de
Barrabin, Hector
author_role author
author2 Rodrigues, Juliany Cola Fernandes
Moreira, Otacilio C.
Atella, Geórgia
Souza, Wanderley de
Barrabin, Hector
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Medina, Jorge Mansur
Rodrigues, Juliany Cola Fernandes
Moreira, Otacilio C.
Atella, Geórgia
Souza, Wanderley de
Barrabin, Hector
dc.subject.en.pt_BR.fl_str_mv Phytomonas
Tomatine
Tomatidine
Lipids
Trypanosomatids
topic Phytomonas
Tomatine
Tomatidine
Lipids
Trypanosomatids
Tomatina
Lipídeos
Trypanosomatina
dc.subject.decs.pt_BR.fl_str_mv Tomatina
Lipídeos
Trypanosomatina
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-11-16T14:49:59Z
dc.date.available.fl_str_mv 2015-11-16T14:49:59Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDINA, Jorge Mansur; et al. Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine. Mem Inst Oswaldo Cruz, v.110, n.1, p. 48-55, Feb. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12240
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760140097
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MEDINA, Jorge Mansur; et al. Mechanisms of growth inhibition of Phytomonas serpens by the alkaloids tomatine and tomatidine. Mem Inst Oswaldo Cruz, v.110, n.1, p. 48-55, Feb. 2015.
0074-0276
10.1590/0074-02760140097
1678-8060
url https://www.arca.fiocruz.br/handle/icict/12240
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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