HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3389/fmicb.2019.00020 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32898 |
Resumo: | Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil. |
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Tanaka, Tayana Serpa OrtizLeite, Thaysse FerreiraFreitas, Solange ZacalusniCesar, Gabriela AlvesRezende, Grazielli Rocha deLindenberg, Andrea de Siqueira CamposGuimarães, Monick LindenmeyerMotta-Castro, Ana Rita Coimbra2019-05-02T11:07:13Z2019-05-02T11:07:13Z2019TANAKA, Tayana Serpa Ortiz et al. HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil. Frontiers in Immunology, v. 10, Article 20, 12p, Jan. 2019.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3289810.3389/fmicb.2019.00020engFrontiers MediaHIVMSMEpidemiologia MolecularRede de transmissãoResistência medicamentosa transmitidaHomens que fazem sexo com homensHIVMSMMolecular epidemiologyTransmitted drug resistanceTransmission networkHIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Hospital Universitário Maria Aparecida Pedrossian. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. FACFAN. Laboratório de Imunologia Clínica. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Hospital Universitário Maria Aparecida Pedrossian. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil / Universidade Federal de Mato Grosso do Sul. FACFAN. Laboratório de Imunologia Clínica. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz. Laboratório de Virologia Molecular. Campo Grande, MS, Brasil.We aimed to characterize HIV-1 molecular epidemiology and transmission clusters among heterosexual (HET) and men who have sex with men (MSM) individuals, as well as transmitted drug resistance mutations (TDRM) in Central-Western Brazil. This cross-sectional survey was conducted among 190 antiretroviral naïve HIV-1 infected individuals. Proviral DNA was extracted, and nested PCR amplified partial polymerase gene (PR/RT). After sequencing, subtypes were assigned, and the sequences were analyzed for the occurrence of possible transmission networks. Calibrated Population Resistance (CPR) tool from Stanford HIV Database was used to investigate the presence of TDRM. Among 150 individuals whose samples were successfully sequenced, the most prevalent HIV-1 subtype was B, followed by recombinant forms. The occurrence of twenty transmission clusters composed by at least two sequences was verified, suggesting the existence of transmission clusters among individuals from the same or distinct sexual orientations. Intermediate level of TDRM (12%) was found in the study population, and almost half of the subjects with TDRM had more than one resistance mutation. No correlations between sexual orientation and the presence of TDRM, HIV-1 subtypes/recombinants forms were verified. Taken together, the necessity of the continuous monitoring of the TDRM to verify the importance of pre-genotyping and to delineate future strategies in primary antiretroviral therapy. Likewise, the knowledge of the HIV-1 transmission networks in Brazil would allow the implementation of effective HIV-1 prevention strategies in local settings.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32898/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdfThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdfapplication/pdf1458462https://www.arca.fiocruz.br/bitstream/icict/32898/2/ThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdf284d2ddea6e30358557b407c22db9188MD52TEXTThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdf.txtThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdf.txtExtracted texttext/plain52261https://www.arca.fiocruz.br/bitstream/icict/32898/3/ThaisseFLeite_AnaRitaCastro_etal_IOC_2019.pdf.txt7356ab89c46e97ad5102bf3f63aedf52MD53icict/328982023-08-03 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| dc.title.pt_BR.fl_str_mv |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| title |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| spellingShingle |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil Tanaka, Tayana Serpa Ortiz HIV MSM Epidemiologia Molecular Rede de transmissão Resistência medicamentosa transmitida Homens que fazem sexo com homens HIV MSM Molecular epidemiology Transmitted drug resistance Transmission network |
| title_short |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| title_full |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| title_fullStr |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| title_full_unstemmed |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| title_sort |
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil |
| author |
Tanaka, Tayana Serpa Ortiz |
| author_facet |
Tanaka, Tayana Serpa Ortiz Leite, Thaysse Ferreira Freitas, Solange Zacalusni Cesar, Gabriela Alves Rezende, Grazielli Rocha de Lindenberg, Andrea de Siqueira Campos Guimarães, Monick Lindenmeyer Motta-Castro, Ana Rita Coimbra |
| author_role |
author |
| author2 |
Leite, Thaysse Ferreira Freitas, Solange Zacalusni Cesar, Gabriela Alves Rezende, Grazielli Rocha de Lindenberg, Andrea de Siqueira Campos Guimarães, Monick Lindenmeyer Motta-Castro, Ana Rita Coimbra |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Tanaka, Tayana Serpa Ortiz Leite, Thaysse Ferreira Freitas, Solange Zacalusni Cesar, Gabriela Alves Rezende, Grazielli Rocha de Lindenberg, Andrea de Siqueira Campos Guimarães, Monick Lindenmeyer Motta-Castro, Ana Rita Coimbra |
| dc.subject.other.pt_BR.fl_str_mv |
HIV MSM Epidemiologia Molecular Rede de transmissão Resistência medicamentosa transmitida Homens que fazem sexo com homens |
| topic |
HIV MSM Epidemiologia Molecular Rede de transmissão Resistência medicamentosa transmitida Homens que fazem sexo com homens HIV MSM Molecular epidemiology Transmitted drug resistance Transmission network |
| dc.subject.en.pt_BR.fl_str_mv |
HIV MSM Molecular epidemiology Transmitted drug resistance Transmission network |
| description |
Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil. |
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2019 |
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2019-05-02T11:07:13Z |
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2019-05-02T11:07:13Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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TANAKA, Tayana Serpa Ortiz et al. HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil. Frontiers in Immunology, v. 10, Article 20, 12p, Jan. 2019. |
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https://www.arca.fiocruz.br/handle/icict/32898 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2019.00020 |
| identifier_str_mv |
TANAKA, Tayana Serpa Ortiz et al. HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil. Frontiers in Immunology, v. 10, Article 20, 12p, Jan. 2019. 1664-302X 10.3389/fmicb.2019.00020 |
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