Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse

Detalhes bibliográficos
Autor(a) principal: Almeida, Daniela Santos
Data de Publicação: 2019
Outros Autores: Paz, Lucas Nogueira, Oliveira, Daiana Santos de, Silva, Danielle Nascimento, Ristow, Paula, Hamond, Camila, Costa, Federico, Portela, Ricardo Wagner, Lima, Alessandra Estrela, Pinna, Melissa Hanzen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60719
Resumo: Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
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spelling Almeida, Daniela SantosPaz, Lucas NogueiraOliveira, Daiana Santos deSilva, Danielle NascimentoRistow, PaulaHamond, CamilaCosta, FedericoPortela, Ricardo WagnerLima, Alessandra EstrelaPinna, Melissa Hanzen2023-10-05T15:59:10Z2023-10-05T15:59:10Z2019ALMEIDA, Daniela Santos et al. investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PLoS ONE, v. 14, n. 5, p. 1-13, 2019.1932-6203https://www.arca.fiocruz.br/handle/icict/6071910.1371/journal.pone.0217391Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil.Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Biologia. Laboratório de Bacteriologia e Saúde. Salvador, BA, Brasil.Plataforma de Salud Animal. Instituto Nacional de Investigacio´n Agropecuaria, INIA La Estanzuela. Colonia, Uruguay.Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Salvador, BA, Brasil.The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse.In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.engPublic Library of ScienceLeptospiraInfecção crônicaOvelhaLeptospiraChronic infectionSheepLeptospiraInfecção PersistenteOvinosInvestigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60719/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlmeida, Daniela S. - Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.pdfAlmeida, Daniela S. - Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.pdfapplication/pdf2302277https://www.arca.fiocruz.br/bitstream/icict/60719/2/Almeida%2c%20Daniela%20S.%20-%20Investigation%20of%20chronic%20infection%20by%20Leptospira%20spp.%20in%20asymptomatic%20sheep%20slaughtered%20in%20slaughterhouse.pdf3027cc0f28e778505499ad96d135dcc5MD52icict/607192023-10-05 12:59:11.361oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-05T15:59:11Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
title Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
spellingShingle Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
Almeida, Daniela Santos
Leptospira
Infecção crônica
Ovelha
Leptospira
Chronic infection
Sheep
Leptospira
Infecção Persistente
Ovinos
title_short Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
title_full Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
title_fullStr Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
title_full_unstemmed Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
title_sort Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
author Almeida, Daniela Santos
author_facet Almeida, Daniela Santos
Paz, Lucas Nogueira
Oliveira, Daiana Santos de
Silva, Danielle Nascimento
Ristow, Paula
Hamond, Camila
Costa, Federico
Portela, Ricardo Wagner
Lima, Alessandra Estrela
Pinna, Melissa Hanzen
author_role author
author2 Paz, Lucas Nogueira
Oliveira, Daiana Santos de
Silva, Danielle Nascimento
Ristow, Paula
Hamond, Camila
Costa, Federico
Portela, Ricardo Wagner
Lima, Alessandra Estrela
Pinna, Melissa Hanzen
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Daniela Santos
Paz, Lucas Nogueira
Oliveira, Daiana Santos de
Silva, Danielle Nascimento
Ristow, Paula
Hamond, Camila
Costa, Federico
Portela, Ricardo Wagner
Lima, Alessandra Estrela
Pinna, Melissa Hanzen
dc.subject.other.en_US.fl_str_mv Leptospira
Infecção crônica
Ovelha
topic Leptospira
Infecção crônica
Ovelha
Leptospira
Chronic infection
Sheep
Leptospira
Infecção Persistente
Ovinos
dc.subject.en.en_US.fl_str_mv Leptospira
Chronic infection
Sheep
dc.subject.decs.en_US.fl_str_mv Leptospira
Infecção Persistente
Ovinos
description Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2023-10-05T15:59:10Z
dc.date.available.fl_str_mv 2023-10-05T15:59:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Daniela Santos et al. investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PLoS ONE, v. 14, n. 5, p. 1-13, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60719
dc.identifier.issn.en_US.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0217391
identifier_str_mv ALMEIDA, Daniela Santos et al. investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PLoS ONE, v. 14, n. 5, p. 1-13, 2019.
1932-6203
10.1371/journal.pone.0217391
url https://www.arca.fiocruz.br/handle/icict/60719
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60719/1/license.txt
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