Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice

Detalhes bibliográficos
Autor(a) principal: Portella, Renata Siqueira
Data de Publicação: 2009
Outros Autores: Andrade, Sonia Gumes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15140
Resumo: Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular. Salvador, BA, Brasil
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spelling Portella, Renata SiqueiraAndrade, Sonia Gumes2016-07-28T13:07:26Z2016-07-28T13:07:26Z2009PORTELLA, R. S.; ANDRADE, S. G. Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice. Memórias do Instituto Oswaldo Cruz, v. 104, n. 7, p. 1023-1030, 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/15140engInstituto Oswaldo CruzTrypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular. Salvador, BA, BrasilWe investigated whether sequestered Trypanosoma cruzi antigens found in heart interstitial dendritic cells (IDCs) contribute to the residual myocarditis found in mice following treatment with benznidazole, a specific chemotherapeutic drug. IDCs are antigen-presenting cells that are MHC-II-receptor dependent. Swiss mice were divided into two experimental groups: the 1st group was infected with the Colombian strain of T. cruzi, which is resistant to treatment with benznidazole, and the 2nd group was infected with clone 21SF-C 3, which has a medium susceptibility to the drug. Treatment of the Colombian strain group started on the 120th day post-infection and for the 21SF-C3 strain group treatment was started on the 90th day. In both groups, treatment lasted for 90 days. The animals were sacrificed either 150 or 200 days post-treatment. The myocardium was analysed by immunohistochemistry using anti-MAC3, 33D1, CD11b and CD11c monoclonal antibodies for IDCs or anti-T. cruzi purified antibodies. Parasite antigens were expressed on the IDC membranes in both treated and untreated mice. Myocarditis subsided following treatment, evidenced by both histological and morphometrical evaluation. A reduction in the number of IDCs carrying T. cruzi antigens in the treated group indicates that the elimination of parasites influences antigen presentation with concomitant decreases in inflammation. There is a correlation between the presence of T. cruzi antigens in these cells and the chronic focal, residual myocarditis seen in treated mice.Trypanosoma cruziChronic myocarditisHeart interstitial dendritic cellsChemotherapyBenznidazoleAnimaisChagas cardiomiopathyDrug resistanceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15140/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPortella SG Trypanosoma cruzi ....pdfPortella SG Trypanosoma cruzi ....pdfapplication/pdf1279477https://www.arca.fiocruz.br/bitstream/icict/15140/2/Portella%20SG%20Trypanosoma%20cruzi%20....pdfd09cd589de469b0a721b9696dd2f8c4dMD52TEXTPortella SG Trypanosoma cruzi ....pdf.txtPortella SG Trypanosoma cruzi ....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
title Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
spellingShingle Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
Portella, Renata Siqueira
Trypanosoma cruzi
Chronic myocarditis
Heart interstitial dendritic cells
Chemotherapy
Benznidazole
Animais
Chagas cardiomiopathy
Drug resistance
title_short Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
title_full Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
title_fullStr Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
title_full_unstemmed Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
title_sort Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice
author Portella, Renata Siqueira
author_facet Portella, Renata Siqueira
Andrade, Sonia Gumes
author_role author
author2 Andrade, Sonia Gumes
author2_role author
dc.contributor.author.fl_str_mv Portella, Renata Siqueira
Andrade, Sonia Gumes
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chronic myocarditis
Heart interstitial dendritic cells
Chemotherapy
Benznidazole
Animais
Chagas cardiomiopathy
Drug resistance
topic Trypanosoma cruzi
Chronic myocarditis
Heart interstitial dendritic cells
Chemotherapy
Benznidazole
Animais
Chagas cardiomiopathy
Drug resistance
description Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular. Salvador, BA, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2016-07-28T13:07:26Z
dc.date.available.fl_str_mv 2016-07-28T13:07:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv PORTELLA, R. S.; ANDRADE, S. G. Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice. Memórias do Instituto Oswaldo Cruz, v. 104, n. 7, p. 1023-1030, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15140
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
identifier_str_mv PORTELLA, R. S.; ANDRADE, S. G. Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting myocarditis in benznidazole-treated mice. Memórias do Instituto Oswaldo Cruz, v. 104, n. 7, p. 1023-1030, 2009.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/15140
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
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