Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit

Detalhes bibliográficos
Autor(a) principal: Migueleti, Deivid L. S.
Data de Publicação: 2012
Outros Autores: Smetana, Juliana H. C., Nunes, Hugo F., Kobarg, Jörg, Zanchin, Nilson Ivo Tonin
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28810
Resumo: Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.
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spelling Migueleti, Deivid L. S.Smetana, Juliana H. C.Nunes, Hugo F.Kobarg, JörgZanchin, Nilson Ivo Tonin2018-09-14T19:47:44Z2018-09-14T19:47:44Z2012MIGUELETI, Deivid L. S. et al. Identification and Characterization of an Alternatively Spliced Isoform of the Human Protein Phosphatase 2Aα Catalytic Subunit. Journal of Biological Chemistry, v. 287, n.7, p. 4853 -4862, 2012.0021-9258https://www.arca.fiocruz.br/handle/icict/2881010.1074/jbc.M111.2833411083-351XporAmerican Society for Biochemistry and Molecular BiologyPP2AProtein Tyrosine PhosphatasesRNA IsoformsHoloenzymesRNAProteínas Tirosina FosfatasasIsoformas de ARNARNProteínas Tirosina FosfatasesIsoformas de RNAHoloenzimasRNA MensageiroIdentification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunitinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.Universidade Estadual de Campinas. Centro de Biologia Molecular e Engenharia Genética, Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.Universidade Estadual de Campinas. Centro de Biologia Molecular e Engenharia Genética, Campinas, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.PP2A is the main serine/threonine-specific phosphatase in animal cells. The active phosphatase has been described as a holoenzyme consisting of a catalytic, a scaffolding, and a variable regulatory subunit, all encoded by multiple genes, allowing for the assembly of more than 70 different holoenzymes. The catalytic subunit can also interact with α4, TIPRL (TIP41, TOR signaling pathway regulator-like), the methyl-transferase LCMT-1, and the methyl-esterase PME-1. Here, we report that the gene encoding the catalytic subunit PP2Acα can generate two mRNA types, the standard mRNA and a shorter isoform, lacking exon 5, which we termed PP2Acα2. Higher levels of the PP2Acα2 mRNA, equivalent to the level of the longer PP2Acα mRNA, were detected in peripheral blood mononuclear cells that were left to rest for 24 h. After this time, the peripheral blood mononuclear cells are still viable and the PP2Acα2 mRNA decreases soon after they are transferred to culture medium, showing that generation of the shorter isoform depends on the incubation conditions. FLAG-tagged PP2Acα2 expressed in HEK293 is catalytically inactive. It displays a specific interaction profile with enhanced binding to the α4 regulatory subunit, but no binding to the scaffolding subunit and PME-1. Consistently, α4 out-competes PME-1 and LCMT-1 for binding to both PP2Acα isoforms in pulldown assays. Together with molecular modeling studies, this suggests that all three regulators share a common binding surface on the catalytic subunit. Our findings add important new insights into the complex mechanisms of PP2A regulation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28810/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALJournalBiolChemistryzb4853.pdfJournalBiolChemistryzb4853.pdfapplication/pdf1990964https://www.arca.fiocruz.br/bitstream/icict/28810/2/JournalBiolChemistryzb4853.pdfbb68ee6b122af42a80fca5054d5c4a3dMD52TEXTJournalBiolChemistryzb4853.pdf.txtJournalBiolChemistryzb4853.pdf.txtExtracted texttext/plain50944https://www.arca.fiocruz.br/bitstream/icict/28810/3/JournalBiolChemistryzb4853.pdf.txt3b480c704446492dbe71882f4e528434MD53icict/288102018-09-19 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dc.title.pt_BR.fl_str_mv Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
title Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
spellingShingle Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
Migueleti, Deivid L. S.
PP2A
Protein Tyrosine Phosphatases
RNA Isoforms
Holoenzymes
RNA
Proteínas Tirosina Fosfatasas
Isoformas de ARN
ARN
Proteínas Tirosina Fosfatases
Isoformas de RNA
Holoenzimas
RNA Mensageiro
title_short Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
title_full Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
title_fullStr Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
title_full_unstemmed Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
title_sort Identification and characterization of an alternatively spliced isoform of the human protein phosphatase 2Aα catalytic subunit
author Migueleti, Deivid L. S.
author_facet Migueleti, Deivid L. S.
Smetana, Juliana H. C.
Nunes, Hugo F.
Kobarg, Jörg
Zanchin, Nilson Ivo Tonin
author_role author
author2 Smetana, Juliana H. C.
Nunes, Hugo F.
Kobarg, Jörg
Zanchin, Nilson Ivo Tonin
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Migueleti, Deivid L. S.
Smetana, Juliana H. C.
Nunes, Hugo F.
Kobarg, Jörg
Zanchin, Nilson Ivo Tonin
dc.subject.other.pt_BR.fl_str_mv PP2A
topic PP2A
Protein Tyrosine Phosphatases
RNA Isoforms
Holoenzymes
RNA
Proteínas Tirosina Fosfatasas
Isoformas de ARN
ARN
Proteínas Tirosina Fosfatases
Isoformas de RNA
Holoenzimas
RNA Mensageiro
dc.subject.en.pt_BR.fl_str_mv Protein Tyrosine Phosphatases
RNA Isoforms
Holoenzymes
RNA
dc.subject.es.pt_BR.fl_str_mv Proteínas Tirosina Fosfatasas
Isoformas de ARN
ARN
dc.subject.decs.pt_BR.fl_str_mv Proteínas Tirosina Fosfatases
Isoformas de RNA
Holoenzimas
RNA Mensageiro
description Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2018-09-14T19:47:44Z
dc.date.available.fl_str_mv 2018-09-14T19:47:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MIGUELETI, Deivid L. S. et al. Identification and Characterization of an Alternatively Spliced Isoform of the Human Protein Phosphatase 2Aα Catalytic Subunit. Journal of Biological Chemistry, v. 287, n.7, p. 4853 -4862, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28810
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.doi.none.fl_str_mv 10.1074/jbc.M111.283341
dc.identifier.eissn.none.fl_str_mv 1083-351X
identifier_str_mv MIGUELETI, Deivid L. S. et al. Identification and Characterization of an Alternatively Spliced Isoform of the Human Protein Phosphatase 2Aα Catalytic Subunit. Journal of Biological Chemistry, v. 287, n.7, p. 4853 -4862, 2012.
0021-9258
10.1074/jbc.M111.283341
1083-351X
url https://www.arca.fiocruz.br/handle/icict/28810
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28810/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28810/2/JournalBiolChemistryzb4853.pdf
https://www.arca.fiocruz.br/bitstream/icict/28810/3/JournalBiolChemistryzb4853.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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