Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test

Detalhes bibliográficos
Autor(a) principal: Gutierrez-Galhardo, Maria C.
Data de Publicação: 2010
Outros Autores: Zancopé-Oliveira, Rosely M., Monzón, Araceli, Rodriguez-Tudela, Juan L., Cuenca-Estrella, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32114
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Gutierrez-Galhardo, Maria C.Zancopé-Oliveira, Rosely M.Monzón, AraceliRodriguez-Tudela, Juan L.Cuenca-Estrella, Manuel2019-03-15T14:53:30Z2019-03-15T14:53:30Z2010GUTIERREZ-GALHARDO, Maria C. et al. Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and e-test. Mycoses, v. 53, p. 227-231, 2009.0933-7407https://www.arca.fiocruz.br/handle/icict/3211410.1111/j.1439-0507.2009.01701.x1439-0507engWileyAntifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.Instituto de Salud Carlos III. Centro Nacional de Microbiologia. Department of Mycology. Madrid, Spain.Instituto de Salud Carlos III. Centro Nacional de Microbiologia. Department of Mycology. Madrid, Spain.Instituto de Salud Carlos III. Centro Nacional de Microbiologia. Department of Mycology. Madrid, Spain.The susceptibility profile of 91 Sporothrix schenckii isolates in both growth phases was determined by microdilution test (Antifungal Susceptibility Testing of the European Committee for Antimicrobial Susceptibility Testing; AFST-EUCAST). Amphotericin B (AMB), itraconazole (ITC), posaconazole, ravuconazole and terbinafine were found active in vitro against both phases but minimum inhibitory concentrations values for mycelial phase were significantly higher. Fluconazole (FLC) and voriconazole (VRC) were inactive in vitro against both phases. The E-test technique was also performed with 41 representative isolates for AMB, FLC, ITC and VRC. Average agreement rates between yeast phase microdilution results and E-test results were high for AMB (77.5%) and FLC (87.8%), but low for ITC and VRC with rates of 56.4% and 54.5%, respectively. AFST-EUCAST is not the most recommended test to perform drug susceptibility testing of S. schenckii in clinical laboratories, and E-test could be an alternative methodology for this purpose, mainly when the activity in vitro of antifungal agents of AMB and FLC are evaluated.E-testGrowth phasesAmphotericin BItraconazoleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32114/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Gutierrez-Galhardo_Maria_etal_INI_2010.pdfve_Gutierrez-Galhardo_Maria_etal_INI_2010.pdfapplication/pdf105630https://www.arca.fiocruz.br/bitstream/icict/32114/2/ve_Gutierrez-Galhardo_Maria_etal_INI_2010.pdf203561681acac490fa31b5e50e54c414MD52TEXTve_Gutierrez-Galhardo_Maria_etal_INI_2010.pdf.txtve_Gutierrez-Galhardo_Maria_etal_INI_2010.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
title Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
spellingShingle Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
Gutierrez-Galhardo, Maria C.
E-test
Growth phases
Amphotericin B
Itraconazole
title_short Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
title_full Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
title_fullStr Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
title_full_unstemmed Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
title_sort Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and E-test
author Gutierrez-Galhardo, Maria C.
author_facet Gutierrez-Galhardo, Maria C.
Zancopé-Oliveira, Rosely M.
Monzón, Araceli
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
author_role author
author2 Zancopé-Oliveira, Rosely M.
Monzón, Araceli
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gutierrez-Galhardo, Maria C.
Zancopé-Oliveira, Rosely M.
Monzón, Araceli
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
dc.subject.en.pt_BR.fl_str_mv E-test
Growth phases
Amphotericin B
Itraconazole
topic E-test
Growth phases
Amphotericin B
Itraconazole
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-03-15T14:53:30Z
dc.date.available.fl_str_mv 2019-03-15T14:53:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUTIERREZ-GALHARDO, Maria C. et al. Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and e-test. Mycoses, v. 53, p. 227-231, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32114
dc.identifier.issn.pt_BR.fl_str_mv 0933-7407
dc.identifier.doi.none.fl_str_mv 10.1111/j.1439-0507.2009.01701.x
dc.identifier.eissn.none.fl_str_mv 1439-0507
identifier_str_mv GUTIERREZ-GALHARDO, Maria C. et al. Antifungal susceptibility profile in vitro of Sporothrix schenckii in two growth phases and by two methods: microdilution and e-test. Mycoses, v. 53, p. 227-231, 2009.
0933-7407
10.1111/j.1439-0507.2009.01701.x
1439-0507
url https://www.arca.fiocruz.br/handle/icict/32114
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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