Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole

Detalhes bibliográficos
Autor(a) principal: Takahagi-Nakaira, E. [UNESP]
Data de Publicação: 2009
Outros Autores: Sugizaki, M. F. [UNESP], Peraçoli, Maria Terezinha S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992009000400010
http://hdl.handle.net/11449/225737
Resumo: In vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.
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spelling Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazoleAmphotericin BAntifungal susceptibilityItraconazoleP. brasiliensisIn vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970Universidade Estadual Paulista (UNESP)Takahagi-Nakaira, E. [UNESP]Sugizaki, M. F. [UNESP]Peraçoli, Maria Terezinha S. [UNESP]2022-04-28T20:59:28Z2022-04-28T20:59:28Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article718-731http://dx.doi.org/10.1590/S1678-91992009000400010Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009.1678-9199http://hdl.handle.net/11449/22573710.1590/S1678-919920090004000102-s2.0-74049083813Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T20:59:28Zoai:repositorio.unesp.br:11449/225737Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:52:02.906533Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
title Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
spellingShingle Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
Takahagi-Nakaira, E. [UNESP]
Amphotericin B
Antifungal susceptibility
Itraconazole
P. brasiliensis
title_short Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
title_full Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
title_fullStr Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
title_full_unstemmed Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
title_sort Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
author Takahagi-Nakaira, E. [UNESP]
author_facet Takahagi-Nakaira, E. [UNESP]
Sugizaki, M. F. [UNESP]
Peraçoli, Maria Terezinha S. [UNESP]
author_role author
author2 Sugizaki, M. F. [UNESP]
Peraçoli, Maria Terezinha S. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Takahagi-Nakaira, E. [UNESP]
Sugizaki, M. F. [UNESP]
Peraçoli, Maria Terezinha S. [UNESP]
dc.subject.por.fl_str_mv Amphotericin B
Antifungal susceptibility
Itraconazole
P. brasiliensis
topic Amphotericin B
Antifungal susceptibility
Itraconazole
P. brasiliensis
description In vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2022-04-28T20:59:28Z
2022-04-28T20:59:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992009000400010
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009.
1678-9199
http://hdl.handle.net/11449/225737
10.1590/S1678-91992009000400010
2-s2.0-74049083813
url http://dx.doi.org/10.1590/S1678-91992009000400010
http://hdl.handle.net/11449/225737
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009.
1678-9199
10.1590/S1678-91992009000400010
2-s2.0-74049083813
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 718-731
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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