SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling

Detalhes bibliográficos
Autor(a) principal: Torices, Silvia
Data de Publicação: 2022
Outros Autores: Motta, Carolline, Rosa, Barbara da, Marcos, Anne, Alvarez-Rosa, Liandra, Siqueira, Michele, Moreno-Rodriguez, Thaidy, Matos, Aline, Caetano, Braulia, Martins, Jessica, Gladulich, Luis, Loiola, Erick, Bagshaw, Olivia, Stuart, Jeffrey, Siqueira, Marilda, Stipursky, Joice, Toborek, Michal, Adesse, Daniel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56903
Resumo: DOI - 10.21203/rs.3.rs-1762855/v1
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spelling Torices, SilviaMotta, CarollineRosa, Barbara daMarcos, AnneAlvarez-Rosa, LiandraSiqueira, MicheleMoreno-Rodriguez, ThaidyMatos, AlineCaetano, BrauliaMartins, JessicaGladulich, LuisLoiola, ErickBagshaw, OliviaStuart, JeffreySiqueira, MarildaStipursky, JoiceToborek, MichalAdesse, Daniel2023-02-06T20:21:41Z2023-02-06T20:21:41Z2022TORICES, Silvia et al. SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling.https://www.arca.fiocruz.br/handle/icict/56903DOI - 10.21203/rs.3.rs-1762855/v1University of Miami Miller School of Medicine.Fundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzUniversidade Federal do Rio de Janeiro.University of California San Francisco. San Fransisco, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundacao Oswaldo CruzFundacao Oswaldo CruzFundacao Oswaldo CruzUniversidade Federal Fluminense. Niterói, RJ, Brasil.Brock University.Brock University.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.University of Miami. USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Neurological effects of COVID-19 and long-COVID-19 as well as neuroinvasion by SARS-CoV-2 still pose several questions and are of both clinical and scientific relevance. We described the cellular and molecular effects of the human brain microvascular endothelial cells (HBMECs) in vitro infection by SARS-CoV-2 to understand the underlying mechanisms of viral transmigration through the Blood-Brain Barrier. Despite the low to non- productive viral replication, SARS-CoV-2-infected cultures displayed increased apoptotic cell death and tight junction protein expression and immunolocalization. Transcriptomic profiling of infected cultures revealed endothelial activation via NF-κB non-canonical pathway, including RELB overexpression, and mitochondrial dysfunction. Additionally, SARS-CoV-2 led to altered secretion of key angiogenic factors and to significant changes in mitochondrial dynamics, with increased mitofusin-2 expression and increased mitochondrial networks. Endothelial activation and remodeling can further contribute to neuroinflammatory processes and lead to further BBB permeability in COVID-19.engResearch SquareInfecção por SARS-CoV-2Cérebro humano microvascularCélulas endoteliais leva à ativação inflamatóriaVia não canônica do NF-κBRemodelação mitocondrialSARS-CoV-2Infection of human brainEndothelial cells leads to inflammatory activationThrough NF-κB non-canonical pathwayMitochondrial remodelingSARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodelinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
title SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
spellingShingle SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
Torices, Silvia
Infecção por SARS-CoV-2
Cérebro humano microvascular
Células endoteliais leva à ativação inflamatória
Via não canônica do NF-κB
Remodelação mitocondrial
SARS-CoV-2
Infection of human brain
Endothelial cells leads to inflammatory activation
Through NF-κB non-canonical pathway
Mitochondrial remodeling
title_short SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
title_full SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
title_fullStr SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
title_full_unstemmed SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
title_sort SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
author Torices, Silvia
author_facet Torices, Silvia
Motta, Carolline
Rosa, Barbara da
Marcos, Anne
Alvarez-Rosa, Liandra
Siqueira, Michele
Moreno-Rodriguez, Thaidy
Matos, Aline
Caetano, Braulia
Martins, Jessica
Gladulich, Luis
Loiola, Erick
Bagshaw, Olivia
Stuart, Jeffrey
Siqueira, Marilda
Stipursky, Joice
Toborek, Michal
Adesse, Daniel
author_role author
author2 Motta, Carolline
Rosa, Barbara da
Marcos, Anne
Alvarez-Rosa, Liandra
Siqueira, Michele
Moreno-Rodriguez, Thaidy
Matos, Aline
Caetano, Braulia
Martins, Jessica
Gladulich, Luis
Loiola, Erick
Bagshaw, Olivia
Stuart, Jeffrey
Siqueira, Marilda
Stipursky, Joice
Toborek, Michal
Adesse, Daniel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torices, Silvia
Motta, Carolline
Rosa, Barbara da
Marcos, Anne
Alvarez-Rosa, Liandra
Siqueira, Michele
Moreno-Rodriguez, Thaidy
Matos, Aline
Caetano, Braulia
Martins, Jessica
Gladulich, Luis
Loiola, Erick
Bagshaw, Olivia
Stuart, Jeffrey
Siqueira, Marilda
Stipursky, Joice
Toborek, Michal
Adesse, Daniel
dc.subject.other.en_US.fl_str_mv Infecção por SARS-CoV-2
Cérebro humano microvascular
Células endoteliais leva à ativação inflamatória
Via não canônica do NF-κB
Remodelação mitocondrial
topic Infecção por SARS-CoV-2
Cérebro humano microvascular
Células endoteliais leva à ativação inflamatória
Via não canônica do NF-κB
Remodelação mitocondrial
SARS-CoV-2
Infection of human brain
Endothelial cells leads to inflammatory activation
Through NF-κB non-canonical pathway
Mitochondrial remodeling
dc.subject.en.en_US.fl_str_mv SARS-CoV-2
Infection of human brain
Endothelial cells leads to inflammatory activation
Through NF-κB non-canonical pathway
Mitochondrial remodeling
description DOI - 10.21203/rs.3.rs-1762855/v1
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-02-06T20:21:41Z
dc.date.available.fl_str_mv 2023-02-06T20:21:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TORICES, Silvia et al. SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56903
identifier_str_mv TORICES, Silvia et al. SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling.
url https://www.arca.fiocruz.br/handle/icict/56903
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Research Square
publisher.none.fl_str_mv Research Square
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56903/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56903/2/MarildaSiqueira_DanielAdesse_etal_IOC_2022.pdf
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