Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.

Detalhes bibliográficos
Autor(a) principal: Carvalho, Kellyanne dos Anjos
Data de Publicação: 2013
Outros Autores: Melo Neto, Osvaldo Pompílio de, Magalhães, Franklin Barbalho, Ponte, João Carlos Marques, Felipe, Filipe Adriano Borba, Santos, Mariese Conceição Alves dos, Lima, Givaneide dos Santos, Cruz Filho, Alvaro Augusto Souza da, Pinheiro, Carina Silva, Pontes-de-Carvalho, Lain Carlos, Neves, Neuza Maria Alcântara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8224
Resumo: Universidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, Brasil
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spelling Carvalho, Kellyanne dos AnjosMelo Neto, Osvaldo Pompílio deMagalhães, Franklin BarbalhoPonte, João Carlos MarquesFelipe, Filipe Adriano BorbaSantos, Mariese Conceição Alves dosLima, Givaneide dos SantosCruz Filho, Alvaro Augusto Souza daPinheiro, Carina SilvaPontes-de-Carvalho, Lain CarlosNeves, Neuza Maria Alcântara2014-08-25T17:06:12Z2014-08-25T17:06:12Z2013CARVALHO, K. dos A. et al. Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract. BMC Immunology, v. 14, p.11, 2013.1471-2172https://www.arca.fiocruz.br/handle/icict/822410.1186/1471-2172-14-11engBioMed CentralBlomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.Associação Caruaruense de Ensino Superior. Caruaru, PE, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Alergia e Acarologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Alergia e Acarologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Salvador, BA, Brasil.Centro Estudos de Alergias Respiratórias. Salvador, BA, Brasil.Centro Estudos de Alergias Respiratórias. Salvador, BA, Brasil / Universidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, Brasil.Universidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, Brasil.BACKGROUND: Blomia tropicalis is a dust mite and an important source of allergens in tropical regions. Up to now, the assays to diagnose atopy to this mite use whole body extract as antigens. However, anti-B. tropicalis IgE antibodies cross-react with Ascaris lumbricoides antigens, hindering the diagnosis of allergy to this mite. In this study, B. tropicalis recombinant allergens were evaluated with the purpose of developing an immunodiagnostic assay for allergy to this mite with greater specificity than those commercially available. METHODS: Two B. tropicalis allergens (Blo t 5 and Blo t 21) were cloned into a plasmidial expression vector, expressed in Escherichia coli and purified by affinity chromatography. Sixty-three sera containing anti-B. tropicalis extract (BtE) IgE antibodies were used to investigate IgE reactivity to the recombinant Blot 5 and 21 allergens. Inhibition assays with 20 sera pre-adsorbed with A. lumbricoides extract were performed using rBlo t 5, rBlo t 21, and BtE as antigens. All the assays were carried using indirect ELISA. RESULTS: Eighty-two point nine percent and 80.0% of the sera with anti-BtE antibodies from 35 children reacted with rBlo t 5 and rBlo t 21, respectively, whereas 92.8% and 89.3% of the 28 sera with anti-BtE antibodies from adult asthma patients reacted with the same allergens, and 96.4% of these sera reacted with a mixture of rBlo t 5 and rBlo t 21. In an inhibition ELISA, the absorption of sera by A. lumbricoides extract affected less the reaction with rBlo t 5 and rBlo t 21 than with BtE. CONCLUSIONS: The rBlo t 5 and rBlo t 21 allergens contain important epitopes recognized by IgE antibodies of individuals allergic to B. tropicalis antigens. Moreover, the assays using the recombinant allergens had lower IgE cross-reactivity with A. lumbricoides antigens, a fact which would confers higher specificity to serodiagnostic assays than the crude mite extract. However, additional recombinant allergens should be evaluated in order to reach the same sensitivity of the commercially available assays based on mite extractBlomia tropicalisRecombinant allergensImmunodiagnosisCross-reactivityAscaris lumbricoidesSensitivitySpecificityAlérgenos/imunologiaAscaris/imunologiaMisturas Complexas/imunologiaÁcaros/imunologiaProteínas Recombinantes de Fusão/imunologiaTestes Sorológicos/métodosAdultoAnimaisAntígenos de Helmintos/imunologiaPré-EscolarReações Cruzadas/imunologiaHumanosImunoglobulina E/imunologiaProteínas Recombinantes de Fusão/isolamento & purificaçãoReprodutibilidade dos TestesSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8224/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALCArvalho KA Blomia tropicalis....pdfCArvalho KA Blomia tropicalis....pdfapplication/pdf663542https://www.arca.fiocruz.br/bitstream/icict/8224/2/CArvalho%20KA%20Blomia%20tropicalis....pdf347309bf124c30836985962ab870eb34MD52TEXTCArvalho KA Blomia tropicalis....pdf.txtCArvalho KA Blomia tropicalis....pdf.txtExtracted texttext/plain43414https://www.arca.fiocruz.br/bitstream/icict/8224/3/CArvalho%20KA%20Blomia%20tropicalis....pdf.txt2f9f635a87b5d884e916f3d618881a24MD53icict/82242023-03-15 14:33:22.069oai:www.arca.fiocruz.br:icict/8224TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
title Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
spellingShingle Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
Carvalho, Kellyanne dos Anjos
Blomia tropicalis
Recombinant allergens
Immunodiagnosis
Cross-reactivity
Ascaris lumbricoides
Sensitivity
Specificity
Alérgenos/imunologia
Ascaris/imunologia
Misturas Complexas/imunologia
Ácaros/imunologia
Proteínas Recombinantes de Fusão/imunologia
Testes Sorológicos/métodos
Adulto
Animais
Antígenos de Helmintos/imunologia
Pré-Escolar
Reações Cruzadas/imunologia
Humanos
Imunoglobulina E/imunologia
Proteínas Recombinantes de Fusão/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
title_short Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
title_full Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
title_fullStr Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
title_full_unstemmed Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
title_sort Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract.
author Carvalho, Kellyanne dos Anjos
author_facet Carvalho, Kellyanne dos Anjos
Melo Neto, Osvaldo Pompílio de
Magalhães, Franklin Barbalho
Ponte, João Carlos Marques
Felipe, Filipe Adriano Borba
Santos, Mariese Conceição Alves dos
Lima, Givaneide dos Santos
Cruz Filho, Alvaro Augusto Souza da
Pinheiro, Carina Silva
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
author_role author
author2 Melo Neto, Osvaldo Pompílio de
Magalhães, Franklin Barbalho
Ponte, João Carlos Marques
Felipe, Filipe Adriano Borba
Santos, Mariese Conceição Alves dos
Lima, Givaneide dos Santos
Cruz Filho, Alvaro Augusto Souza da
Pinheiro, Carina Silva
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Kellyanne dos Anjos
Melo Neto, Osvaldo Pompílio de
Magalhães, Franklin Barbalho
Ponte, João Carlos Marques
Felipe, Filipe Adriano Borba
Santos, Mariese Conceição Alves dos
Lima, Givaneide dos Santos
Cruz Filho, Alvaro Augusto Souza da
Pinheiro, Carina Silva
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
dc.subject.en.pt_BR.fl_str_mv Blomia tropicalis
Recombinant allergens
Immunodiagnosis
Cross-reactivity
Ascaris lumbricoides
Sensitivity
Specificity
topic Blomia tropicalis
Recombinant allergens
Immunodiagnosis
Cross-reactivity
Ascaris lumbricoides
Sensitivity
Specificity
Alérgenos/imunologia
Ascaris/imunologia
Misturas Complexas/imunologia
Ácaros/imunologia
Proteínas Recombinantes de Fusão/imunologia
Testes Sorológicos/métodos
Adulto
Animais
Antígenos de Helmintos/imunologia
Pré-Escolar
Reações Cruzadas/imunologia
Humanos
Imunoglobulina E/imunologia
Proteínas Recombinantes de Fusão/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
dc.subject.decs.pt_BR.fl_str_mv Alérgenos/imunologia
Ascaris/imunologia
Misturas Complexas/imunologia
Ácaros/imunologia
Proteínas Recombinantes de Fusão/imunologia
Testes Sorológicos/métodos
Adulto
Animais
Antígenos de Helmintos/imunologia
Pré-Escolar
Reações Cruzadas/imunologia
Humanos
Imunoglobulina E/imunologia
Proteínas Recombinantes de Fusão/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
description Universidade Federal da Bahia. ProAR Núcleo de Excelência em Asma. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-08-25T17:06:12Z
dc.date.available.fl_str_mv 2014-08-25T17:06:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, K. dos A. et al. Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract. BMC Immunology, v. 14, p.11, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8224
dc.identifier.issn.none.fl_str_mv 1471-2172
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2172-14-11
identifier_str_mv CARVALHO, K. dos A. et al. Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract. BMC Immunology, v. 14, p.11, 2013.
1471-2172
10.1186/1471-2172-14-11
url https://www.arca.fiocruz.br/handle/icict/8224
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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