Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12429 |
Resumo: | p. 1-9 |
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Carvalho, Kellyanne dos AnjosMelo Neto, Osvaldo Pompílio deMagalhães, Franklin BarbalhoPonte, João Carlos MarquesFelipe, Filipe Adriano BorbaSantos, Mariese Conceição Alves dosLima, Givaneide dos SantosCruz Filho, Álvaro Augusto Souza daPinheiro, Carina SilvaCarvalho, Lain Carlos Pontes deAlcântara-Neves, Neuza MariaCarvalho, Kellyanne dos AnjosMelo Neto, Osvaldo Pompílio deMagalhães, Franklin BarbalhoPonte, João Carlos MarquesFelipe, Filipe Adriano BorbaSantos, Mariese Conceição Alves dosLima, Givaneide dos SantosCruz Filho, Álvaro Augusto Souza daPinheiro, Carina SilvaCarvalho, Lain Carlos Pontes deAlcântara-Neves, Neuza Maria2013-08-05T01:23:24Z2013-08-05T01:23:24Z20131471-2172http://www.repositorio.ufba.br/ri/handle/ri/12429v. 14, n. 11p. 1-9Background: Blomia tropicalis is a dust mite and an important source of allergens in tropical regions. Up to now, the assays to diagnose atopy to this mite use whole body extract as antigens. However, anti-B. tropicalis IgE antibodies cross-react with Ascaris lumbricoides antigens, hindering the diagnosis of allergy to this mite. In this study, B. tropicalis recombinant allergens were evaluated with the purpose of developing an immunodiagnostic assay for allergy to this mite with greater specificity than those commercially available. Methods: Two B. tropicalis allergens (Blo t 5 and Blo t 21) were cloned into a plasmidial expression vector, expressed in Escherichia coli and purified by affinity chromatography. Sixty-three sera containing anti-B. tropicalis extract (BtE) IgE antibodies were used to investigate IgE reactivity to the recombinant Blot 5 and 21 allergens. Inhibition assays with 20 sera pre-adsorbed with A. lumbricoides extract were performed using rBlo t 5, rBlo t 21, and BtE as antigens. All the assays were carried using indirect ELISA. Results: Eighty-two point nine percent and 80.0% of the sera with anti-BtE antibodies from 35 children reacted with rBlo t 5 and rBlo t 21, respectively, whereas 92.8% and 89.3% of the 28 sera with anti-BtE antibodies from adult asthma patients reacted with the same allergens, and 96.4% of these sera reacted with a mixture of rBlo t 5 and rBlo t 21. In an inhibition ELISA, the absorption of sera by A. lumbricoides extract affected less the reaction with rBlo t 5 and rBlo t 21 than with BtE. Conclusions: The rBlo t 5 and rBlo t 21 allergens contain important epitopes recognized by IgE antibodies of individuals allergic to B. tropicalis antigens. Moreover, the assays using the recombinant allergens had lower IgE cross-reactivity with A. lumbricoides antigens, a fact which would confers higher specificity to serodiagnostic assays than the crude mite extract. However, additional recombinant allergens should be evaluated in order to reach the same sensitivity of the commercially available assays based on mite extract.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-01T18:37:27Z No. of bitstreams: 1 4444444444.pdf: 663543 bytes, checksum: 50cd2955ae422d4a820260a0a7f9d281 (MD5)Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-08-05T01:23:24Z (GMT) No. of bitstreams: 1 4444444444.pdf: 663543 bytes, checksum: 50cd2955ae422d4a820260a0a7f9d281 (MD5)Made available in DSpace on 2013-08-05T01:23:24Z (GMT). No. of bitstreams: 1 4444444444.pdf: 663543 bytes, checksum: 50cd2955ae422d4a820260a0a7f9d281 (MD5) Previous issue date: 2013SalvadorBMC Immunology10.1186/1471-2172-14-11reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABlomia tropicalisRecombinant allergensImmunodiagnosisCross-reactivityAscaris lumbricoidesSensitivitySpecificityBlomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extractBMC Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL4444444444.pdf4444444444.pdfapplication/pdf663543https://repositorio.ufba.br/bitstream/ri/12429/1/4444444444.pdf50cd2955ae422d4a820260a0a7f9d281MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12429/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT4444444444.pdf.txt4444444444.pdf.txtExtracted texttext/plain43402https://repositorio.ufba.br/bitstream/ri/12429/3/4444444444.pdf.txtd0f00b2a62c6dbdc612c40b8488f4a9bMD53ri/124292022-08-08 13:17:43.202oai:repositorio.ufba.br:ri/12429VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:43Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| dc.title.alternative.pt_BR.fl_str_mv |
BMC Immunology |
| title |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| spellingShingle |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract Carvalho, Kellyanne dos Anjos Blomia tropicalis Recombinant allergens Immunodiagnosis Cross-reactivity Ascaris lumbricoides Sensitivity Specificity |
| title_short |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| title_full |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| title_fullStr |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| title_full_unstemmed |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| title_sort |
Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract |
| author |
Carvalho, Kellyanne dos Anjos |
| author_facet |
Carvalho, Kellyanne dos Anjos Melo Neto, Osvaldo Pompílio de Magalhães, Franklin Barbalho Ponte, João Carlos Marques Felipe, Filipe Adriano Borba Santos, Mariese Conceição Alves dos Lima, Givaneide dos Santos Cruz Filho, Álvaro Augusto Souza da Pinheiro, Carina Silva Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria |
| author_role |
author |
| author2 |
Melo Neto, Osvaldo Pompílio de Magalhães, Franklin Barbalho Ponte, João Carlos Marques Felipe, Filipe Adriano Borba Santos, Mariese Conceição Alves dos Lima, Givaneide dos Santos Cruz Filho, Álvaro Augusto Souza da Pinheiro, Carina Silva Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Kellyanne dos Anjos Melo Neto, Osvaldo Pompílio de Magalhães, Franklin Barbalho Ponte, João Carlos Marques Felipe, Filipe Adriano Borba Santos, Mariese Conceição Alves dos Lima, Givaneide dos Santos Cruz Filho, Álvaro Augusto Souza da Pinheiro, Carina Silva Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria Carvalho, Kellyanne dos Anjos Melo Neto, Osvaldo Pompílio de Magalhães, Franklin Barbalho Ponte, João Carlos Marques Felipe, Filipe Adriano Borba Santos, Mariese Conceição Alves dos Lima, Givaneide dos Santos Cruz Filho, Álvaro Augusto Souza da Pinheiro, Carina Silva Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria |
| dc.subject.por.fl_str_mv |
Blomia tropicalis Recombinant allergens Immunodiagnosis Cross-reactivity Ascaris lumbricoides Sensitivity Specificity |
| topic |
Blomia tropicalis Recombinant allergens Immunodiagnosis Cross-reactivity Ascaris lumbricoides Sensitivity Specificity |
| description |
p. 1-9 |
| publishDate |
2013 |
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2013-08-05T01:23:24Z |
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2013-08-05T01:23:24Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/12429 |
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1471-2172 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 14, n. 11 |
| identifier_str_mv |
1471-2172 v. 14, n. 11 |
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http://www.repositorio.ufba.br/ri/handle/ri/12429 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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BMC Immunology |
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BMC Immunology |
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10.1186/1471-2172-14-11 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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