Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2012
Outros Autores: Carneiro, Fabiana A., Martins, Ivo C., Miranda, Iranaia Assunção, Faustino, André F., Pereira, Renata M., Bozza, Patrícia T., Castanho, Miguel A. R. B., Borges, Ronaldo Mohana, Poian, Andrea T. da, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23019
Resumo: Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.
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spelling Carvalho, Filomena A.Carneiro, Fabiana A.Martins, Ivo C.Miranda, Iranaia AssunçãoFaustino, André F.Pereira, Renata M.Bozza, Patrícia T.Castanho, Miguel A. R. B.Borges, Ronaldo MohanaPoian, Andrea T. daSantos, Nuno C.2017-11-01T13:15:02Z2017-11-01T13:15:02Z2012CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012.0022-538Xhttps://www.arca.fiocruz.br/handle/icict/2301910.1128/JVI.06796-111098-5514engAmerican Society for MicrobiologyVírus da dengueProteínas de MembranaPotássioLípido hepáticoDengue virusHepatic Lipid DropletsLD Surface ProteinsPotassiumDengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Pólo Avançado de Xerém. Duque de Caxias, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.Dengue virus (DENV) affects millions of people, causing more than 20,000 deaths annually. No effective treatment for the disease caused by DENV infection is currently available, partially due to the lack of knowledge on the basic aspects of the viral life cycle, including the molecular basis of the interaction between viral components and cellular compartments. Here, we characterized the properties of the interaction between the DENV capsid (C) protein and hepatic lipid droplets (LDs), which was recently shown to be essential for the virus replication cycle. Zeta potential analysis revealed a negative surface charge of LDs, with an average surface charge of -19 mV. The titration of LDs with C protein led to an increase of the surface charge, which reached a plateau at +13.7 mV, suggesting that the viral protein-LD interaction exposes the protein cationic surface to the aqueous environment. Atomic force microscopy (AFM)-based force spectroscopy measurements were performed by using C protein-functionalized AFM tips. The C protein-LD interaction was found to be strong, with a single (un)binding force of 33.6 pN. This binding was dependent on high intracellular concentrations of potassium ions but not sodium. The inhibition of Na(+)/K(+)-ATPase in DENV-infected cells resulted in the dissociation of C protein from LDs and a 50-fold inhibition of infectious virus production but not of RNA replication, indicating a biological relevance for the potassium-dependent interaction. Limited proteolysis of the LD surface impaired the C protein-LD interaction, and force measurements in the presence of specific antibodies indicated that perilipin 3 (TIP47) is the major DENV C protein ligand on the surface of LDs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23019/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpatricia2_bozza_etal_IOC_2012.pdfpatricia2_bozza_etal_IOC_2012.pdfapplication/pdf2219358https://www.arca.fiocruz.br/bitstream/icict/23019/2/patricia2_bozza_etal_IOC_2012.pdfd2e446a37fd88dd259d30d4e8f256da4MD52TEXTpatricia2_bozza_etal_IOC_2012.pdf.txtpatricia2_bozza_etal_IOC_2012.pdf.txtExtracted texttext/plain74265https://www.arca.fiocruz.br/bitstream/icict/23019/3/patricia2_bozza_etal_IOC_2012.pdf.txt500f6a63c141c677644bd0c1440e8041MD53icict/230192023-09-05 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dc.title.pt_BR.fl_str_mv Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
spellingShingle Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
Carvalho, Filomena A.
Vírus da dengue
Proteínas de Membrana
Potássio
Lípido hepático
Dengue virus
Hepatic Lipid Droplets
LD Surface Proteins
Potassium
title_short Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_full Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_fullStr Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_full_unstemmed Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_sort Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Carneiro, Fabiana A.
Martins, Ivo C.
Miranda, Iranaia Assunção
Faustino, André F.
Pereira, Renata M.
Bozza, Patrícia T.
Castanho, Miguel A. R. B.
Borges, Ronaldo Mohana
Poian, Andrea T. da
Santos, Nuno C.
author_role author
author2 Carneiro, Fabiana A.
Martins, Ivo C.
Miranda, Iranaia Assunção
Faustino, André F.
Pereira, Renata M.
Bozza, Patrícia T.
Castanho, Miguel A. R. B.
Borges, Ronaldo Mohana
Poian, Andrea T. da
Santos, Nuno C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Carneiro, Fabiana A.
Martins, Ivo C.
Miranda, Iranaia Assunção
Faustino, André F.
Pereira, Renata M.
Bozza, Patrícia T.
Castanho, Miguel A. R. B.
Borges, Ronaldo Mohana
Poian, Andrea T. da
Santos, Nuno C.
dc.subject.other.pt_BR.fl_str_mv Vírus da dengue
Proteínas de Membrana
Potássio
Lípido hepático
topic Vírus da dengue
Proteínas de Membrana
Potássio
Lípido hepático
Dengue virus
Hepatic Lipid Droplets
LD Surface Proteins
Potassium
dc.subject.en.pt_BR.fl_str_mv Dengue virus
Hepatic Lipid Droplets
LD Surface Proteins
Potassium
description Universidade de Lisboa. Faculdade de Medicina. Instituto de Medicina Molecular. Lisboa, Portugal.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-11-01T13:15:02Z
dc.date.available.fl_str_mv 2017-11-01T13:15:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23019
dc.identifier.issn.pt_BR.fl_str_mv 0022-538X
dc.identifier.doi.none.fl_str_mv 10.1128/JVI.06796-11
dc.identifier.eissn.none.fl_str_mv 1098-5514
identifier_str_mv CARVALHO, Filomena A. et al. Dengue Virus Capsid Protein Binding to Hepatic Lipid Droplets (LD) Is Potassium Ion Dependent and Is Mediated by LD Surface Proteins. Journal of Virology, v.86, n.4, p.2096-2108, Feb. 2012.
0022-538X
10.1128/JVI.06796-11
1098-5514
url https://www.arca.fiocruz.br/handle/icict/23019
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23019/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23019/2/patricia2_bozza_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/23019/3/patricia2_bozza_etal_IOC_2012.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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