Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2012
Outros Autores: Carneiro, Fabiana A., Martins, Ivo C., Assunção-Miranda, Iranaia, Faustino, André F., Pereira, Renata M., Bozza, Patricia T., Castanho, Miguel A. R. B., Mohana-Borges, Ronaldo, Poian, Andrea T. Da, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10765
Resumo: Copyright © 2012, American Society for Microbiology. All Rights Reserved.
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spelling Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteinsCopyright © 2012, American Society for Microbiology. All Rights Reserved.Dengue virus (DENV) affects millions of people, causing more than 20,000 deaths annually. No effective treatment for the disease caused by DENV infection is currently available, partially due to the lack of knowledge on the basic aspects of the viral life cycle, including the molecular basis of the interaction between viral components and cellular compartments. Here, we characterized the properties of the interaction between the DENV capsid (C) protein and hepatic lipid droplets (LDs), which was recently shown to be essential for the virus replication cycle. Zeta potential analysis revealed a negative surface charge of LDs, with an average surface charge of -19 mV. The titration of LDs with C protein led to an increase of the surface charge, which reached a plateau at +13.7 mV, suggesting that the viral protein-LD interaction exposes the protein cationic surface to the aqueous environment. Atomic force microscopy (AFM)-based force spectroscopy measurements were performed by using C protein-functionalized AFM tips. The C protein-LD interaction was found to be strong, with a single (un)binding force of 33.6 pN. This binding was dependent on high intracellular concentrations of potassium ions but not sodium. The inhibition of Na+/K+-ATPase in DENV-infected cells resulted in the dissociation of C protein from LDs and a 50-fold inhibition of infectious virus production but not of RNA replication, indicating a biological relevance for the potassium-dependent interaction. Limited proteolysis of the LD surface impaired the C protein-LD interaction, and force measurements in the presence of specific antibodies indicated that perilipin 3 (TIP47) is the major DENV C protein ligand on the surface of LDs.This work was supported by FP7-PEOPLE IRSES (International Research Staff Exchange Scheme) project MEMPEPACROSS (European Union), by the Fundação para a Ciência e a Tecnologia—Ministério da Educação e Ciência (FCT-MEC, Portugal) (projects PTDC/QUI-BIQ/112929/2009 and PTDC/QUI/69937/2006), by Fundação Calouste Gulbenkian (Portugal), by the FCT-CAPES Portugal-Brazil joint cooperation projects, and by the Brazilian funding agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo a` Pesquisa do Estado do Rio de Janeiro (FAPERJ), Financiadora de Estudos e Projetos (FINEP), and National Institute of Science and Technology in Dengue (INCT-Dengue). I. C. Martins also acknowledges consecutive postdoctoral funding from a Marie Curie International Outgoing Fellowship (MC-IOF-237373) and FCT-MEC postdoctoral fellowships (SFRH/BPD/46324/2008 and SFRH/BPD/74287/2010). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.American Society for MicrobiologyRepositório da Universidade de LisboaCarvalho, Filomena A.Carneiro, Fabiana A.Martins, Ivo C.Assunção-Miranda, IranaiaFaustino, André F.Pereira, Renata M.Bozza, Patricia T.Castanho, Miguel A. R. B.Mohana-Borges, RonaldoPoian, Andrea T. DaSantos, Nuno C.2014-03-21T11:42:07Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10765engJournal of Virology, 2012, p. 2096–21080022-538Xhttp://dx.doi.org/10.1128/JVI.06796-11info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:29Zoai:repositorio.ul.pt:10451/10765Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:41.206201Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
spellingShingle Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
Carvalho, Filomena A.
title_short Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_full Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_fullStr Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_full_unstemmed Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
title_sort Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Carneiro, Fabiana A.
Martins, Ivo C.
Assunção-Miranda, Iranaia
Faustino, André F.
Pereira, Renata M.
Bozza, Patricia T.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Poian, Andrea T. Da
Santos, Nuno C.
author_role author
author2 Carneiro, Fabiana A.
Martins, Ivo C.
Assunção-Miranda, Iranaia
Faustino, André F.
Pereira, Renata M.
Bozza, Patricia T.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Poian, Andrea T. Da
Santos, Nuno C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Carneiro, Fabiana A.
Martins, Ivo C.
Assunção-Miranda, Iranaia
Faustino, André F.
Pereira, Renata M.
Bozza, Patricia T.
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
Poian, Andrea T. Da
Santos, Nuno C.
description Copyright © 2012, American Society for Microbiology. All Rights Reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-21T11:42:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10765
url http://hdl.handle.net/10451/10765
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Virology, 2012, p. 2096–2108
0022-538X
http://dx.doi.org/10.1128/JVI.06796-11
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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