An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy

Detalhes bibliográficos
Autor(a) principal: Chimelli, L. M.
Data de Publicação: 2006
Outros Autores: Valentim, V. C., Corte-Real, S., Sarno, Euzenir N., Jardim, M. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38816
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Chimelli, L. M.Valentim, V. C.Corte-Real, S.Sarno, Euzenir N.Jardim, M. R.2019-12-26T11:26:36Z2019-12-26T11:26:36Z2006ANTUNES, S. L. G. et al. An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy. Brazilian Journal of Medical and Biological Research, v. 39, p. 1071-1081, 2006.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/3881610.1590/S0100-879X20060008000101414-431XengAssociação Brasileira de Divulgação CientíficaProteína de neurofilamentoReceptor do fator de crescimento nervosoProteína básica de mielinaProduto genético 9.5Hanseníase neuríticaProteína S-100Neurofilament proteinNerve growth factor receptorS-100 proteinMyelin basic proteinGene product 9.5Neuritic leprosyAn immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.The nerve biopsies of 11 patients with pure neuritic leprosy were submitted to routine diagnostic procedures and immunoperoxidase staining with antibodies against axonal (neurofilament, nerve growth factor receptor (NGFr), and protein gene product (PGP) 9.5) and Schwann cell (myelin basic protein, S-100 protein, and NGFr) markers. Two pairs of non-adjacent histological cross-sections of the peripheral nerve were removed for quantification. All the fascicles of the nerve were examined with a 10X-ocular and 40X-objective lens. The immunohistochemistry results were compared to the results of semithin section analysis and clinical and electroneuromyographic data. Neurofilament staining was reduced in 100% of the neuritic biopsies. NGFr positivity was also reduced in 81.8%, PGP staining in 100% of the affected nerves, S100 positivity in 90.9%, and myelin basic protein immunoreactivity in 90.9%. Hypoesthesia was associated with decreased NGFr (81.8%) and PGP staining (90.9%). Reduced potential amplitudes (electroneuromyographic data) were found to be associated with reduced PGP 9.5 (63.6%) and nerve fiber neurofilament staining (45.4%) by immunohistochemistry and with loss of myelinated fibers (100%) by semithin section analysis. On the other hand, the small fibers (immunoreactive dots) seen amid inflammatory cells continued to be present even after 40% of the larger myelinated fibers had disappeared. The present study shows an indepth view of the destructive effects of leprosy upon the expression of neural markers and the integrity of nerve fiber. The association of these structural changes with the clinical and electroneuromyographic manifestations of leprosy peripheral neuropathy was also discusse.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/38816/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSLGAntunes_VCValentim_etal_IOC_2006.pdfSLGAntunes_VCValentim_etal_IOC_2006.pdfapplication/pdf2420298https://www.arca.fiocruz.br/bitstream/icict/38816/2/SLGAntunes_VCValentim_etal_IOC_2006.pdfbfd3eed971abe7065d4a48c3b48f9d6bMD52TEXTSLGAntunes_VCValentim_etal_IOC_2006.pdf.txtSLGAntunes_VCValentim_etal_IOC_2006.pdf.txtExtracted texttext/plain37877https://www.arca.fiocruz.br/bitstream/icict/38816/3/SLGAntunes_VCValentim_etal_IOC_2006.pdf.txt68df082e018a39dfc86a5a5e69d58ec7MD53icict/388162019-12-27 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dc.title.pt_BR.fl_str_mv An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
title An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
spellingShingle An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
Chimelli, L. M.
Proteína de neurofilamento
Receptor do fator de crescimento nervoso
Proteína básica de mielina
Produto genético 9.5
Hanseníase neurítica
Proteína S-100
Neurofilament protein
Nerve growth factor receptor
S-100 protein
Myelin basic protein
Gene product 9.5
Neuritic leprosy
title_short An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
title_full An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
title_fullStr An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
title_full_unstemmed An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
title_sort An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy
author Chimelli, L. M.
author_facet Chimelli, L. M.
Valentim, V. C.
Corte-Real, S.
Sarno, Euzenir N.
Jardim, M. R.
author_role author
author2 Valentim, V. C.
Corte-Real, S.
Sarno, Euzenir N.
Jardim, M. R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chimelli, L. M.
Valentim, V. C.
Corte-Real, S.
Sarno, Euzenir N.
Jardim, M. R.
dc.subject.other.pt_BR.fl_str_mv Proteína de neurofilamento
Receptor do fator de crescimento nervoso
Proteína básica de mielina
Produto genético 9.5
Hanseníase neurítica
Proteína S-100
topic Proteína de neurofilamento
Receptor do fator de crescimento nervoso
Proteína básica de mielina
Produto genético 9.5
Hanseníase neurítica
Proteína S-100
Neurofilament protein
Nerve growth factor receptor
S-100 protein
Myelin basic protein
Gene product 9.5
Neuritic leprosy
dc.subject.en.pt_BR.fl_str_mv Neurofilament protein
Nerve growth factor receptor
S-100 protein
Myelin basic protein
Gene product 9.5
Neuritic leprosy
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2019-12-26T11:26:36Z
dc.date.available.fl_str_mv 2019-12-26T11:26:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANTUNES, S. L. G. et al. An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy. Brazilian Journal of Medical and Biological Research, v. 39, p. 1071-1081, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38816
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/S0100-879X2006000800010
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv ANTUNES, S. L. G. et al. An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy. Brazilian Journal of Medical and Biological Research, v. 39, p. 1071-1081, 2006.
0100-879X
10.1590/S0100-879X2006000800010
1414-431X
url https://www.arca.fiocruz.br/handle/icict/38816
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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