Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella

Detalhes bibliográficos
Autor(a) principal: Baschirotto, Priscila Tonon
Data de Publicação: 2017
Outros Autores: Krieger, Marco Aurélio, Foti, Leonardo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20776
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
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spelling Baschirotto, Priscila TononKrieger, Marco AurélioFoti, Leonardo2017-08-28T13:40:36Z2017-08-28T13:40:36Z2017BASCHIROTTO, Priscila T.; KRIEGER, Marco Aurélio; FOTI, Leonardo. Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella. Memórias do Instituto Oswaldo Cruz, v.112, n.6, p. 428-436, 2017.1678-8060https://www.arca.fiocruz.br/handle/icict/20776porFundação Oswaldo CruzRubéolaToxoplasmosisRubellaDiagnosisImmunoglobulin GToxoplasmosisInmunoglobulina GToxoplasmoseDiagnósticoImunoglobulina GPreliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubellainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. This assay is based on xMap technology. Initially, by singleplex assays, we evaluated the following antigens: one Toxoplasma gondii lysate; two antigenic extracts of T. gondii (TOX8131 and TOX8122); fragments of T. gondii antigens [SAG-1 (amino acids 45-198), GRA-7 (24-100), GRA-1 (57-149), ROP-4, and MIC-3 (234-306)]; two chimeric antigens composed of fragments of SAG-1, GRA-7, and P35 (CTOX and CTOXH); and fragments of Rubella virus antigens [E-1 (157-176, 213-239, 374-390), E-2 (31-105), and C (1-123)]. A multiplex assay to simultaneously diagnose toxoplasmosis and rubella was designed with the best-performing antigens in singleplex and multiplex assays, which included CTOXH, T. gondii lysate, TOX8131, E-1, and E-2. The multiplex assay showed 100% sensitivity and specificity for anti-T. gondii IgG detection and 95.6% sensitivity and 100% specificity for anti-R. virus IgG detection. We found that, despite the difficulties related to developing multiplex systems, different types of antigens (extracts and recombinant proteins) can be used to develop high-performance diagnostic tests. The assay developed is suitable to screen for prior T. gondii and R. virus infections, because it is a rapid, high-throughput, low-cost alternative to the current standard diagnostic tools, which require multiple individual tests.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20776/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALBaschirottoPrelim.pdfBaschirottoPrelim.pdfapplication/pdf383790https://www.arca.fiocruz.br/bitstream/icict/20776/2/BaschirottoPrelim.pdff962c7e28eda5a6c9268b2028085983aMD52TEXTBaschirottoPrelim.pdf.txtBaschirottoPrelim.pdf.txtExtracted texttext/plain46403https://www.arca.fiocruz.br/bitstream/icict/20776/3/BaschirottoPrelim.pdf.txt2c6c6e2228cf369eb7b6f0d4c561f8b0MD53icict/207762021-12-23 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dc.title.pt_BR.fl_str_mv Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
title Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
spellingShingle Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
Baschirotto, Priscila Tonon
Rubéola
Toxoplasmosis
Rubella
Diagnosis
Immunoglobulin G
Toxoplasmosis
Inmunoglobulina G
Toxoplasmose
Diagnóstico
Imunoglobulina G
title_short Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
title_full Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
title_fullStr Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
title_full_unstemmed Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
title_sort Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
author Baschirotto, Priscila Tonon
author_facet Baschirotto, Priscila Tonon
Krieger, Marco Aurélio
Foti, Leonardo
author_role author
author2 Krieger, Marco Aurélio
Foti, Leonardo
author2_role author
author
dc.contributor.author.fl_str_mv Baschirotto, Priscila Tonon
Krieger, Marco Aurélio
Foti, Leonardo
dc.subject.other.pt_BR.fl_str_mv Rubéola
topic Rubéola
Toxoplasmosis
Rubella
Diagnosis
Immunoglobulin G
Toxoplasmosis
Inmunoglobulina G
Toxoplasmose
Diagnóstico
Imunoglobulina G
dc.subject.en.pt_BR.fl_str_mv Toxoplasmosis
Rubella
Diagnosis
Immunoglobulin G
dc.subject.es.pt_BR.fl_str_mv Toxoplasmosis
Inmunoglobulina G
dc.subject.decs.pt_BR.fl_str_mv Toxoplasmose
Diagnóstico
Imunoglobulina G
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-08-28T13:40:36Z
dc.date.available.fl_str_mv 2017-08-28T13:40:36Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BASCHIROTTO, Priscila T.; KRIEGER, Marco Aurélio; FOTI, Leonardo. Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella. Memórias do Instituto Oswaldo Cruz, v.112, n.6, p. 428-436, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20776
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
identifier_str_mv BASCHIROTTO, Priscila T.; KRIEGER, Marco Aurélio; FOTI, Leonardo. Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella. Memórias do Instituto Oswaldo Cruz, v.112, n.6, p. 428-436, 2017.
1678-8060
url https://www.arca.fiocruz.br/handle/icict/20776
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20776/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/20776/2/BaschirottoPrelim.pdf
https://www.arca.fiocruz.br/bitstream/icict/20776/3/BaschirottoPrelim.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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