Axenic amastigotes of Leishmania species as a suitable model for in vitro studies

Detalhes bibliográficos
Autor(a) principal: Lopes, Geovane Dias
Data de Publicação: 2021
Outros Autores: Zabala-Penafiel, Anabel, Melo, Barbara Cristina de Albuquerque, Silva, Franklin Souza, Canto, Laura Menaguali do, Cysne-Finkelstein, Léa, Alves, Carlos Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47881
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
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spelling Lopes, Geovane DiasZabala-Penafiel, AnabelMelo, Barbara Cristina de AlbuquerqueSilva, Franklin SouzaCanto, Laura Menaguali doCysne-Finkelstein, LéaAlves, Carlos Roberto2021-06-26T18:08:35Z2021-06-26T18:08:35Z2021LOPES, Geovane Dias et al. Axenic amastigotes of Leishmania species as a suitable model for in vitro studies . Acta tropica, v. 220, 105956, 14 p, May 2021.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/4788110.1016/j.actatropica.2021.105956engElsevierLeishmaniaCultura in vitroTriagem de drogasAvaliação Pré-Clínica de MedicamentosLeishmaniaAxenic amastigoteAmastigote-likeIn vitro cultureDrug screeningAvaliação Pré-Clínica de MedicamentosAxenic amastigotes of Leishmania species as a suitable model for in vitro studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. / Universidade Iguaçu. Faculdade de Ciências Biológicas e da Saúde. Nova Iguaçu, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Leishmania spp. are etiological agents of infection diseases, which in some cases can be fatal. The main forms of their biological cycle, promastigotes and amastigotes, can be maintained in vitro. While promastigotes are easier to maintain, amastigotes are more complex and can be obtained through different ways, including infection assays of tissues or in vitro cells, and differentiation from promastigotes to axenic amastigotes. Several protocols have been proposed for in vitro differentiation for at least 12 Leishmania spp. of both subgenera, Leishmania and Viannia. In this review we propose a critical summary of axenic amastigotes induction, as well as the impact of these strategies on metabolic pathways and regulatory networks analyzed by omics approaches. The parameters used by different research groups show considerable variations in temperature, pH and induction stages, as highlighted here for Leishmania (Viannia) braziliensis. Therefore, a consensus on strategies for inducing amasti gogenesis is necessary to improve accuracy and even define stage-specific biomarkers. In fact, the axenic amastigote model has contributed to elucidate several aspects of the parasite cycle, however, since it does not reproduce the intracellular environment, its use requires several precautions. In addition, we present a discussion about using axenic amastigotes for drug screening, suggesting the need of a more sensitive methodology to verify cell viability in these tests. Collectively, this review explores the advantages and limitations found in studies with axenic amastigotes, done for more than 30 years, and discuss the gaps that impair their use as a suitable model for in vitro studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47881/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGeovaneLopes_etal_IOC_2021.pdfGeovaneLopes_etal_IOC_2021.pdfapplication/pdf1019619https://www.arca.fiocruz.br/bitstream/icict/47881/2/GeovaneLopes_etal_IOC_2021.pdfb246dceac18a8d92f357b315a6de66a3MD52TEXTGeovaneLopes_etal_IOC_2021.pdf.txtGeovaneLopes_etal_IOC_2021.pdf.txtExtracted texttext/plain101542https://www.arca.fiocruz.br/bitstream/icict/47881/3/GeovaneLopes_etal_IOC_2021.pdf.txt05cfe58b601da87aba1dc5e9a66747c3MD53icict/478812021-06-27 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dc.title.pt_BR.fl_str_mv Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
title Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
spellingShingle Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
Lopes, Geovane Dias
Leishmania
Cultura in vitro
Triagem de drogas
Avaliação Pré-Clínica de Medicamentos
Leishmania
Axenic amastigote
Amastigote-like
In vitro culture
Drug screening
Avaliação Pré-Clínica de Medicamentos
title_short Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
title_full Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
title_fullStr Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
title_full_unstemmed Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
title_sort Axenic amastigotes of Leishmania species as a suitable model for in vitro studies
author Lopes, Geovane Dias
author_facet Lopes, Geovane Dias
Zabala-Penafiel, Anabel
Melo, Barbara Cristina de Albuquerque
Silva, Franklin Souza
Canto, Laura Menaguali do
Cysne-Finkelstein, Léa
Alves, Carlos Roberto
author_role author
author2 Zabala-Penafiel, Anabel
Melo, Barbara Cristina de Albuquerque
Silva, Franklin Souza
Canto, Laura Menaguali do
Cysne-Finkelstein, Léa
Alves, Carlos Roberto
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, Geovane Dias
Zabala-Penafiel, Anabel
Melo, Barbara Cristina de Albuquerque
Silva, Franklin Souza
Canto, Laura Menaguali do
Cysne-Finkelstein, Léa
Alves, Carlos Roberto
dc.subject.other.pt_BR.fl_str_mv Leishmania
Cultura in vitro
Triagem de drogas
Avaliação Pré-Clínica de Medicamentos
topic Leishmania
Cultura in vitro
Triagem de drogas
Avaliação Pré-Clínica de Medicamentos
Leishmania
Axenic amastigote
Amastigote-like
In vitro culture
Drug screening
Avaliação Pré-Clínica de Medicamentos
dc.subject.en.pt_BR.fl_str_mv Leishmania
Axenic amastigote
Amastigote-like
In vitro culture
Drug screening
dc.subject.decs.pt_BR.fl_str_mv Avaliação Pré-Clínica de Medicamentos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-26T18:08:35Z
dc.date.available.fl_str_mv 2021-06-26T18:08:35Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOPES, Geovane Dias et al. Axenic amastigotes of Leishmania species as a suitable model for in vitro studies . Acta tropica, v. 220, 105956, 14 p, May 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47881
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2021.105956
identifier_str_mv LOPES, Geovane Dias et al. Axenic amastigotes of Leishmania species as a suitable model for in vitro studies . Acta tropica, v. 220, 105956, 14 p, May 2021.
0001-706X
10.1016/j.actatropica.2021.105956
url https://www.arca.fiocruz.br/handle/icict/47881
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/47881/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/47881/2/GeovaneLopes_etal_IOC_2021.pdf
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