In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Meira, Cássio Santana
Data de Publicação: 2015
Outros Autores: Guimarãesa, Elisalva Teixeira, Santos, Jamyle Andrade Ferreira dos, Moreira, Diogo Rodrigo Magalhães, Nogueira, Renata Campos, Tomassini, Therezinha Coelho Barbosa, Ribeiro, Ivone Maria, Souza, Claudia Valeria Campos de, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13713
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Meira, Cássio SantanaGuimarãesa, Elisalva TeixeiraSantos, Jamyle Andrade Ferreira dosMoreira, Diogo Rodrigo MagalhãesNogueira, Renata CamposTomassini, Therezinha Coelho BarbosaRibeiro, Ivone MariaSouza, Claudia Valeria Campos deSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2016-04-12T13:34:21Z2016-04-12T13:34:21Z2015MEIRA, C. S. et al. In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi. Phytomedicine, v. 22, n. 11, p. 969-974, 2015.1618-095Xhttps://www.arca.fiocruz.br/handle/icict/13713dx.doi.org/10.1016/j.phymed.2015.07.004engElsevierIn vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Salvador, BA,BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Salvador, BA,BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2-Extração, Isolamento e Purificação. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2-Extração, Isolamento e Purificação. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2-Extração, Isolamento e Purificação. Rio de Janeiro, RJ, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilBackground:ThecurrenttreatmentofChagasdisease,endemicinLatinAmericaandemerginginseveralcountries,islimitedbythefrequentsideeffectsandvariableefficacyofbenznidazole.Naturalproductsareanimportantsourceforthesearchfornewdrugs.Aim/hypothesis:Consideringthegreatpotentialofnaturalproductsasantiparasiticagents,weinvestigatedtheanti-TrypanosomacruziactivityofaconcentratedethanolicextractofPhysalisangulata(EEPA).Methods:Cytotoxicitytomammaliancellswasdeterminedusingmouseperitonealmacrophages.Thean-tiparasiticactivitywasevaluatedagainstaxenicepimastigoteandbloodstreamtrypomastigoteformsofT.cruzi,andagainstamastigoteformsusingT.cruzi-infectedmacrophages.Celldeathmechanismwasde-terminedintrypomastigotesbyflowcytometryanalysisafterannexinVandpropidiumiodidestaining.TheefficacyofEEPAwasexaminedinvivoinanacutemodelofinfectionbymonitoringbloodparasitaemiaandsurvivalrate30daysaftertreatment.TheeffectagainsttrypomastigotesofEEPAandbenznidazoleactingincombinationwasevaluated.Results:EEPAeffectivelyinhibitstheepimastigotegrowth(IC502.9±0.1μM)andreducesbloodstreamtry-pomastigoteviability(EC501.7±0.5μM).Itcausesparasitecelldeathbynecrosis.EEPAimpairsparasiteinfectivityaswellasamastigotedevelopmentinconcentrationsnoncytotoxictomammaliancells.Inmiceacutely-infectedwithT.cruzi,EEPAreducedthebloodparasitaemiain72.7%.Whencombinedwithbenznida-zole,EEPAshowedasynergisticanti-T.cruziactivity,displayingCIvaluesof0.8±0.07atEC50and0.83±0.1atEC90.Conclusion:EEPAhasantiparasiticactivityagainstT.cruzi,causingcelldeathbynecrosisandshowingsyner-gisticactivitywithbenznidazole.ThesefindingswerereinforcedbytheobservedefficacyofEEPAinreducingparasiteloadinT.cruzi-mice.Therefore,thisrepresentsanimportantsourceofantiparasiticnaturaChagas diseaseTrypanosoma cruziPhysalis angulataPlant extractDrug combinationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
title In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
spellingShingle In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
Meira, Cássio Santana
Chagas disease
Trypanosoma cruzi
Physalis angulata
Plant extract
Drug combination
title_short In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
title_full In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
title_fullStr In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
title_full_unstemmed In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
title_sort In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi
author Meira, Cássio Santana
author_facet Meira, Cássio Santana
Guimarãesa, Elisalva Teixeira
Santos, Jamyle Andrade Ferreira dos
Moreira, Diogo Rodrigo Magalhães
Nogueira, Renata Campos
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Souza, Claudia Valeria Campos de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author_role author
author2 Guimarãesa, Elisalva Teixeira
Santos, Jamyle Andrade Ferreira dos
Moreira, Diogo Rodrigo Magalhães
Nogueira, Renata Campos
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Souza, Claudia Valeria Campos de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Meira, Cássio Santana
Guimarãesa, Elisalva Teixeira
Santos, Jamyle Andrade Ferreira dos
Moreira, Diogo Rodrigo Magalhães
Nogueira, Renata Campos
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Souza, Claudia Valeria Campos de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
dc.subject.en.pt_BR.fl_str_mv Chagas disease
Trypanosoma cruzi
Physalis angulata
Plant extract
Drug combination
topic Chagas disease
Trypanosoma cruzi
Physalis angulata
Plant extract
Drug combination
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-12T13:34:21Z
dc.date.available.fl_str_mv 2016-04-12T13:34:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEIRA, C. S. et al. In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi. Phytomedicine, v. 22, n. 11, p. 969-974, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13713
dc.identifier.issn.none.fl_str_mv 1618-095X
dc.identifier.doi.none.fl_str_mv dx.doi.org/10.1016/j.phymed.2015.07.004
identifier_str_mv MEIRA, C. S. et al. In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi. Phytomedicine, v. 22, n. 11, p. 969-974, 2015.
1618-095X
dx.doi.org/10.1016/j.phymed.2015.07.004
url https://www.arca.fiocruz.br/handle/icict/13713
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13713/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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