Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).

Detalhes bibliográficos
Autor(a) principal: Ferraz, Rosana Paula Cruz
Data de Publicação: 2013
Outros Autores: Bomfim, Diogo S, Carvalho, Nanashara Coelho de, Soares, Milena Botelho Pereira, Silva, Thanany Brasil da, Machado, Wedna de Jesus, Prata, Ana Paula do Nascimento, Costa, Emmanoel Vilaça, Moraes, Valéria Regina de Souza, Nogueira, Paulo Cesar de Lima, Bezerra, Daniel Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8553
Resumo: Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil
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spelling Ferraz, Rosana Paula CruzBomfim, Diogo SCarvalho, Nanashara Coelho deSoares, Milena Botelho PereiraSilva, Thanany Brasil daMachado, Wedna de JesusPrata, Ana Paula do NascimentoCosta, Emmanoel VilaçaMoraes, Valéria Regina de SouzaNogueira, Paulo Cesar de LimaBezerra, Daniel Pereira2014-10-08T18:53:10Z2014-10-08T18:53:10Z2013FERRAZ, R. P. C. et al. Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). Phytomedicine, v. 20, n. 7, p. 615-621, 2013.1618-095Xhttps://www.arca.fiocruz.br/handle/icict/855310.1016/j.phymed.2013.01.015engElsevierCytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Biology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Biology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilMedicinal plants are one of the most important sources of drugs used in the pharmaceutical industry. Among traditional medicinal plants, Lippia gracilis Schauer (Verbenaceae) had been used for several medicinal purposes in Brazilian northeastern. In this study, leaf essential oil (EO) of L. gracilis was prepared using hydrodistillation. Followed by GC–MS analysis, its composition was characterized by the presence of thymol (55.50%), as major constituent. The effects of EO on cell proliferation and apoptosis induction were investigated in HepG2 cells. Furthermore, mice bearing Sarcoma 180 tumor cells were used to confirm its in vivo effectiveness. EO and its constituents (thymol, p-cymene, -terpinene and myrcene) displayed cytotoxicity to different tumor cell lines. EO treatment caused G1 arrest in HepG2 cells accompanied by the induction of DNA fragmentation without affecting cell membrane integrity. Cell morphology consistent with apoptosis and a remarkable activation of caspase-3 were also observed, suggesting induction of caspase-dependent apoptotic cell death. In vivo antitumor study showed tumor growth inhibition rates of 38.5–41.9%. In conclusion, the tested essential oil of L. gracilis leaves, which has thymol as its major constituent, possesses significant in vitro and in vivo antitumor activity. These data suggest that leaf essential oil of L. gracilis is a potential medicinal resource.Lippia gracilisEssential oilCytotoxicityApoptosisAntitumorAntineoplásicos Fitogênicos/farmacologiaLippia/químicaÓleos Voláteis/farmacologiaAlcenos/farmacologiaAnimaisBrasilLinhagem Celular Tumoral/efeitos de drogasMembrana Celular/efeitos de drogasCromatografia Gasosa-Espectrometria de MassasCélulas Hep G2/efeitos de drogasHumanosMasculinoCamundongosMonoterpenos/farmacologiaÓleos Voláteis/análiseFolhas de Planta/químicaPlantas Medicinais/químicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8553/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALFerraz RPC Cytotoxic effect....pdfFerraz RPC Cytotoxic effect....pdfapplication/pdf518077https://www.arca.fiocruz.br/bitstream/icict/8553/2/Ferraz%20RPC%20Cytotoxic%20effect....pdf7e9ab563e64c540459b870f256a3843aMD52TEXTFerraz RPC Cytotoxic effect....pdf.txtFerraz RPC Cytotoxic effect....pdf.txtExtracted texttext/plain39454https://www.arca.fiocruz.br/bitstream/icict/8553/3/Ferraz%20RPC%20Cytotoxic%20effect....pdf.txt210b7e3db8e01077cc859c332508fadeMD53icict/85532023-03-15 14:32:54.919oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
title Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
spellingShingle Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
Ferraz, Rosana Paula Cruz
Lippia gracilis
Essential oil
Cytotoxicity
Apoptosis
Antitumor
Antineoplásicos Fitogênicos/farmacologia
Lippia/química
Óleos Voláteis/farmacologia
Alcenos/farmacologia
Animais
Brasil
Linhagem Celular Tumoral/efeitos de drogas
Membrana Celular/efeitos de drogas
Cromatografia Gasosa-Espectrometria de Massas
Células Hep G2/efeitos de drogas
Humanos
Masculino
Camundongos
Monoterpenos/farmacologia
Óleos Voláteis/análise
Folhas de Planta/química
Plantas Medicinais/química
title_short Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
title_full Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
title_fullStr Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
title_full_unstemmed Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
title_sort Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).
author Ferraz, Rosana Paula Cruz
author_facet Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Silva, Thanany Brasil da
Machado, Wedna de Jesus
Prata, Ana Paula do Nascimento
Costa, Emmanoel Vilaça
Moraes, Valéria Regina de Souza
Nogueira, Paulo Cesar de Lima
Bezerra, Daniel Pereira
author_role author
author2 Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Silva, Thanany Brasil da
Machado, Wedna de Jesus
Prata, Ana Paula do Nascimento
Costa, Emmanoel Vilaça
Moraes, Valéria Regina de Souza
Nogueira, Paulo Cesar de Lima
Bezerra, Daniel Pereira
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Silva, Thanany Brasil da
Machado, Wedna de Jesus
Prata, Ana Paula do Nascimento
Costa, Emmanoel Vilaça
Moraes, Valéria Regina de Souza
Nogueira, Paulo Cesar de Lima
Bezerra, Daniel Pereira
dc.subject.en.pt_BR.fl_str_mv Lippia gracilis
Essential oil
Cytotoxicity
Apoptosis
Antitumor
topic Lippia gracilis
Essential oil
Cytotoxicity
Apoptosis
Antitumor
Antineoplásicos Fitogênicos/farmacologia
Lippia/química
Óleos Voláteis/farmacologia
Alcenos/farmacologia
Animais
Brasil
Linhagem Celular Tumoral/efeitos de drogas
Membrana Celular/efeitos de drogas
Cromatografia Gasosa-Espectrometria de Massas
Células Hep G2/efeitos de drogas
Humanos
Masculino
Camundongos
Monoterpenos/farmacologia
Óleos Voláteis/análise
Folhas de Planta/química
Plantas Medicinais/química
dc.subject.decs.pt_BR.fl_str_mv Antineoplásicos Fitogênicos/farmacologia
Lippia/química
Óleos Voláteis/farmacologia
Alcenos/farmacologia
Animais
Brasil
Linhagem Celular Tumoral/efeitos de drogas
Membrana Celular/efeitos de drogas
Cromatografia Gasosa-Espectrometria de Massas
Células Hep G2/efeitos de drogas
Humanos
Masculino
Camundongos
Monoterpenos/farmacologia
Óleos Voláteis/análise
Folhas de Planta/química
Plantas Medicinais/química
description Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-08T18:53:10Z
dc.date.available.fl_str_mv 2014-10-08T18:53:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRAZ, R. P. C. et al. Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). Phytomedicine, v. 20, n. 7, p. 615-621, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8553
dc.identifier.issn.none.fl_str_mv 1618-095X
dc.identifier.doi.none.fl_str_mv 10.1016/j.phymed.2013.01.015
identifier_str_mv FERRAZ, R. P. C. et al. Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae). Phytomedicine, v. 20, n. 7, p. 615-621, 2013.
1618-095X
10.1016/j.phymed.2013.01.015
url https://www.arca.fiocruz.br/handle/icict/8553
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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