Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23551 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil |
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Sequeira, Danielly C. M. deMenezes, Rodrigo C.Oliveira, Manoel M. E.Antas, Paulo R. Z.De Luca, Paula M.Ferreira, Joseli de OliveiraBorba, Cintia de Moraes2017-12-07T16:09:33Z2017-12-07T16:09:33Z2017SEQUEIRA Danielly C. M. et al. Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models. Frontiers in Microbiology, v.8, Article 1617, 13p, Aug. 2017.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/2355110.3389/fmicb.2017.01617engFrontiers MediaModelos TeóricosHialoifomicoseImunossupressãoResposta imunePurpureocillium lilacinumhyalohyphomycosisexperimental modelimmune responseimmunosuppressionExperimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Modelsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Purpureocillium lilacinum is a filamentous, hyaline fungus considered an emerging pathogen in humans. The aim of our study was to evaluate the outcome of hyalohyphomycosis in C57BL/6 murine models inoculated with two clinical P. lilacinum isolates (S1 and S2). Each isolate was inoculated in mice randomly distributed in immunocompetent (CPT) and immunosuppressed (SPS) groups. Mice were evaluated at day 7, 21, and 45 after inoculation for histopathological analysis, recovery of fungal cells, and immunological studies. Histological analysis showed scarce conidia-like structures in lung tissue from CPT mice and a lot of fungal cells in SPS mice inoculated with S2 compared to mice inoculated with S1. The maximum recovery of fungal cells was seen in CPT mice inoculated with both isolates at day 7, but with mean significantly higher in those inoculated with S2 isolate. Phenotypical characterization of T cells showed TCD8+ lymphocytes predominance over TCD4+ in immunosuppressed mice infected and control groups. We also observed higher percentages of the central and effector memory/effector phenotype in CPT mice infected with S2 strain, especially in TCD8+ in the initial period of infection. Regulatory T cells showed higher percentages in immunosuppressed, predominantly after the acute phase. Our results showed that the P. lilacinum is a fungus capable to cause damages in competent and immunosuppressed experimental hosts. Furthermore, S2 isolate seems to cause more damage to the experimental host and it was possible to identify different cellular subsets involved in the mice immune response.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23551/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdanielly_sequeira_etal_IOC_2017.pdfdanielly_sequeira_etal_IOC_2017.pdfapplication/pdf743680https://www.arca.fiocruz.br/bitstream/icict/23551/2/danielly_sequeira_etal_IOC_2017.pdfd2bb294ec0541cbb1e8380120659e380MD52TEXTdanielly_sequeira_etal_IOC_2017.pdf.txtdanielly_sequeira_etal_IOC_2017.pdf.txtExtracted texttext/plain59821https://www.arca.fiocruz.br/bitstream/icict/23551/3/danielly_sequeira_etal_IOC_2017.pdf.txt4757641c3997b86660adc021c105f115MD53icict/235512023-09-05 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| dc.title.pt_BR.fl_str_mv |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| title |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| spellingShingle |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models Sequeira, Danielly C. M. de Modelos Teóricos Hialoifomicose Imunossupressão Resposta imune Purpureocillium lilacinum hyalohyphomycosis experimental model immune response immunosuppression |
| title_short |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| title_full |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| title_fullStr |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| title_full_unstemmed |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| title_sort |
Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models |
| author |
Sequeira, Danielly C. M. de |
| author_facet |
Sequeira, Danielly C. M. de Menezes, Rodrigo C. Oliveira, Manoel M. E. Antas, Paulo R. Z. De Luca, Paula M. Ferreira, Joseli de Oliveira Borba, Cintia de Moraes |
| author_role |
author |
| author2 |
Menezes, Rodrigo C. Oliveira, Manoel M. E. Antas, Paulo R. Z. De Luca, Paula M. Ferreira, Joseli de Oliveira Borba, Cintia de Moraes |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Sequeira, Danielly C. M. de Menezes, Rodrigo C. Oliveira, Manoel M. E. Antas, Paulo R. Z. De Luca, Paula M. Ferreira, Joseli de Oliveira Borba, Cintia de Moraes |
| dc.subject.other.pt_BR.fl_str_mv |
Modelos Teóricos Hialoifomicose Imunossupressão Resposta imune |
| topic |
Modelos Teóricos Hialoifomicose Imunossupressão Resposta imune Purpureocillium lilacinum hyalohyphomycosis experimental model immune response immunosuppression |
| dc.subject.en.pt_BR.fl_str_mv |
Purpureocillium lilacinum hyalohyphomycosis experimental model immune response immunosuppression |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil |
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2017 |
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2017-12-07T16:09:33Z |
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2017-12-07T16:09:33Z |
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2017 |
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info:eu-repo/semantics/article |
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SEQUEIRA Danielly C. M. et al. Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models. Frontiers in Microbiology, v.8, Article 1617, 13p, Aug. 2017. |
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https://www.arca.fiocruz.br/handle/icict/23551 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2017.01617 |
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SEQUEIRA Danielly C. M. et al. Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models. Frontiers in Microbiology, v.8, Article 1617, 13p, Aug. 2017. 1664-302X 10.3389/fmicb.2017.01617 |
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