Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17914 |
Resumo: | Fundação de Amparo a Pesquisa do Estado de Minas Gerais. Belo Horizonte, MG, Brazil |
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Resende, Lucilene AparecidaSoares, Rodrigo Dian de Oliveira AguiarKer, Henrique GamaRoatt, Bruno MendesMendonça, Ludmila Zanandreis deAlves, Marina Luiza RodriguesLemos, Denise da SilveiraOliveira, Rodrigo Corrêa deMartins Filho, Olindo AssisAraújo, Márcio Sobreira SilvaFujiwara, Ricardo ToshioGontijo, Nelder FigueiredoReis, Alexandre BarbosaGiunchetti, Rodolfo Cordeiro2017-03-02T17:21:03Z2017-03-02T17:21:03Z2016RESENDE, Lucilene Aparecida et al. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. PLoS One, v. 11, n. 8, art. e0161169, 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1791410.1371/journal.pone.0161169Fundação de Amparo a Pesquisa do Estado de Minas Gerais. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil/Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilDogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection.engPublic Library of ScienceLeishmaniose visceral caninaLutzomyia longipalpisImpact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challengeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/17914/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Resende_Lucilene_Impact of LbSapSal_CPqRR_2016.pdfve_Resende_Lucilene_Impact of 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| dc.title.pt_BR.fl_str_mv |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| title |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| spellingShingle |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge Resende, Lucilene Aparecida Leishmaniose visceral canina Lutzomyia longipalpis |
| title_short |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| title_full |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| title_fullStr |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| title_full_unstemmed |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| title_sort |
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| author |
Resende, Lucilene Aparecida |
| author_facet |
Resende, Lucilene Aparecida Soares, Rodrigo Dian de Oliveira Aguiar Ker, Henrique Gama Roatt, Bruno Mendes Mendonça, Ludmila Zanandreis de Alves, Marina Luiza Rodrigues Lemos, Denise da Silveira Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva Fujiwara, Ricardo Toshio Gontijo, Nelder Figueiredo Reis, Alexandre Barbosa Giunchetti, Rodolfo Cordeiro |
| author_role |
author |
| author2 |
Soares, Rodrigo Dian de Oliveira Aguiar Ker, Henrique Gama Roatt, Bruno Mendes Mendonça, Ludmila Zanandreis de Alves, Marina Luiza Rodrigues Lemos, Denise da Silveira Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva Fujiwara, Ricardo Toshio Gontijo, Nelder Figueiredo Reis, Alexandre Barbosa Giunchetti, Rodolfo Cordeiro |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Resende, Lucilene Aparecida Soares, Rodrigo Dian de Oliveira Aguiar Ker, Henrique Gama Roatt, Bruno Mendes Mendonça, Ludmila Zanandreis de Alves, Marina Luiza Rodrigues Lemos, Denise da Silveira Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva Fujiwara, Ricardo Toshio Gontijo, Nelder Figueiredo Reis, Alexandre Barbosa Giunchetti, Rodolfo Cordeiro |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose visceral canina Lutzomyia longipalpis |
| topic |
Leishmaniose visceral canina Lutzomyia longipalpis |
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Fundação de Amparo a Pesquisa do Estado de Minas Gerais. Belo Horizonte, MG, Brazil |
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2016 |
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2016 |
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2017-03-02T17:21:03Z |
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2017-03-02T17:21:03Z |
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RESENDE, Lucilene Aparecida et al. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. PLoS One, v. 11, n. 8, art. e0161169, 2016. |
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https://www.arca.fiocruz.br/handle/icict/17914 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0161169 |
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RESENDE, Lucilene Aparecida et al. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. PLoS One, v. 11, n. 8, art. e0161169, 2016. 1932-6203 10.1371/journal.pone.0161169 |
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Public Library of Science |
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