Potential biomarkers for the clinical prognosis of severe dengue

Silva, Mayara Marques Carneiro da; Gil, Laura Helena Vega Gonzales; Marques, Ernesto Torres de Azevedo; Calzavara-Silva, Carlos Eduardo

Potential biomarkers for the clinical prognosis of severe dengue

Detalhes bibliográficos
Autor(a) principal:	Silva, Mayara Marques Carneiro da
Data de Publicação:	2013
Outros Autores:	Gil, Laura Helena Vega Gonzales, Marques, Ernesto Torres de Azevedo, Calzavara-Silva, Carlos Eduardo
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/23617
Resumo:	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.

Metadados do item

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spelling	Silva, Mayara Marques Carneiro daGil, Laura Helena Vega GonzalesMarques, Ernesto Torres de AzevedoCalzavara-Silva, Carlos Eduardo2017-12-12T12:41:16Z2017-12-12T12:41:16Z2013SILVA, M. M. C. da et al. Potential biomarkers for the clinical prognosis of severe dengue. Memorias Do Instituto Oswaldo Cruz, v. 108, n. 6, p. 755–762, set. 2013.1678-8060https://www.arca.fiocruz.br/handle/icict/2361710.1590/0074-0276108062013012engdenguebiomarcadoresapoptoseimunidade inataPCR quantitativa em tempo realDenguebiomarkersapoptosisinnate immunityquantitative real-time PCRAntígenos de NeoplasiasgenéticaCisteína EndopeptidasesgenéticaGlicoproteínas de MembranagenéticaProteínas de NeoplasiasgenéticaPeroxirredoxinasgenéticaReceptores de IgGgenéticaReceptores de Interleucina-1genéticaDengue GravediagnósticoÍndice de Gravidade de DoençaBiomarcadoresProteínas Reguladoras de ApoptosegenéticaProteínas Ligadas por GPIgenéticaExpressão GênicaHumanosImunidade InatagenéticaLeucócitos MononuclearesimunologiaLeucócitos MononuclearespatologiaAnálise em MicrossériesPrognósticoRNA Mensageiroisolamento & purificaçãoReação em Cadeia da Polimerase em Tempo RealSorotipagemPotential biomarkers for the clinical prognosis of severe dengueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, PA, USA.Fundação Oswaldo Cruz. Instituto René Rachou. Departamento de Imunologia. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.Currently, several assays can confirm acute dengue infection at the point-of-care. However, none of these assays can predict the severity of the disease symptoms. A prognosis test that predicts the likelihood of a dengue patient to develop a severe form of the disease could permit more efficient patient triage and treatment. We hypothesise that mRNA expression of apoptosis and innate immune response-related genes will be differentially regulated during the early stages of dengue and might predict the clinical outcome. Aiming to identify biomarkers for dengue prognosis, we extracted mRNA from the peripheral blood mononuclear cells of mild and severe dengue patients during the febrile stage of the disease to measure the expression levels of selected genes by quantitative polymerase chain reaction. The selected candidate biomarkers were previously identified by our group as differentially expressed in microarray studies. We verified that the mRNA coding for CFD, MAGED1, PSMB9, PRDX4 and FCGR3B were differentially expressed between patients who developed clinical symptoms associated with the mild type of dengue and patients who showed clinical symptoms associated with severe dengue. We suggest that this gene expression panel could putatively serve as biomarkers for the clinical prognosis of dengue haemorrhagic fever.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23617/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. Potential biomarkers - silva.pdfart. Potential biomarkers - silva.pdfapplication/pdf490671https://www.arca.fiocruz.br/bitstream/icict/23617/2/art.%20Potential%20biomarkers%20-%20silva.pdfa2f8dc14474b0b5a323d5f7228580167MD52TEXTart. Potential biomarkers - silva.pdf.txtart. 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dc.title.pt_BR.fl_str_mv	Potential biomarkers for the clinical prognosis of severe dengue
title	Potential biomarkers for the clinical prognosis of severe dengue
spellingShingle	Potential biomarkers for the clinical prognosis of severe dengue Silva, Mayara Marques Carneiro da dengue biomarcadores apoptose imunidade inata PCR quantitativa em tempo real Dengue biomarkers apoptosis innate immunity quantitative real-time PCR Antígenos de Neoplasias genética Cisteína Endopeptidases genética Glicoproteínas de Membrana genética Proteínas de Neoplasias genética Peroxirredoxinas genética Receptores de IgG genética Receptores de Interleucina-1 genética Dengue Grave diagnóstico Índice de Gravidade de Doença Biomarcadores Proteínas Reguladoras de Apoptose genética Proteínas Ligadas por GPI genética Expressão Gênica Humanos Imunidade Inata genética Leucócitos Mononucleares imunologia Leucócitos Mononucleares patologia Análise em Microsséries Prognóstico RNA Mensageiro isolamento & purificação Reação em Cadeia da Polimerase em Tempo Real Sorotipagem
title_short	Potential biomarkers for the clinical prognosis of severe dengue
title_full	Potential biomarkers for the clinical prognosis of severe dengue
title_fullStr	Potential biomarkers for the clinical prognosis of severe dengue
title_full_unstemmed	Potential biomarkers for the clinical prognosis of severe dengue
title_sort	Potential biomarkers for the clinical prognosis of severe dengue
author	Silva, Mayara Marques Carneiro da
author_facet	Silva, Mayara Marques Carneiro da Gil, Laura Helena Vega Gonzales Marques, Ernesto Torres de Azevedo Calzavara-Silva, Carlos Eduardo
author_role	author
author2	Gil, Laura Helena Vega Gonzales Marques, Ernesto Torres de Azevedo Calzavara-Silva, Carlos Eduardo
author2_role	author author author
dc.contributor.author.fl_str_mv	Silva, Mayara Marques Carneiro da Gil, Laura Helena Vega Gonzales Marques, Ernesto Torres de Azevedo Calzavara-Silva, Carlos Eduardo
dc.subject.other.pt_BR.fl_str_mv	dengue biomarcadores apoptose imunidade inata PCR quantitativa em tempo real
topic	dengue biomarcadores apoptose imunidade inata PCR quantitativa em tempo real Dengue biomarkers apoptosis innate immunity quantitative real-time PCR Antígenos de Neoplasias genética Cisteína Endopeptidases genética Glicoproteínas de Membrana genética Proteínas de Neoplasias genética Peroxirredoxinas genética Receptores de IgG genética Receptores de Interleucina-1 genética Dengue Grave diagnóstico Índice de Gravidade de Doença Biomarcadores Proteínas Reguladoras de Apoptose genética Proteínas Ligadas por GPI genética Expressão Gênica Humanos Imunidade Inata genética Leucócitos Mononucleares imunologia Leucócitos Mononucleares patologia Análise em Microsséries Prognóstico RNA Mensageiro isolamento & purificação Reação em Cadeia da Polimerase em Tempo Real Sorotipagem
dc.subject.en.pt_BR.fl_str_mv	Dengue biomarkers apoptosis innate immunity quantitative real-time PCR
dc.subject.decs.pt_BR.fl_str_mv	Antígenos de Neoplasias genética Cisteína Endopeptidases genética Glicoproteínas de Membrana genética Proteínas de Neoplasias genética Peroxirredoxinas genética Receptores de IgG genética Receptores de Interleucina-1 genética Dengue Grave diagnóstico Índice de Gravidade de Doença Biomarcadores Proteínas Reguladoras de Apoptose genética Proteínas Ligadas por GPI genética Expressão Gênica Humanos Imunidade Inata genética Leucócitos Mononucleares imunologia Leucócitos Mononucleares patologia Análise em Microsséries Prognóstico RNA Mensageiro isolamento & purificação Reação em Cadeia da Polimerase em Tempo Real Sorotipagem
description	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.
publishDate	2013
dc.date.issued.fl_str_mv	2013
dc.date.accessioned.fl_str_mv	2017-12-12T12:41:16Z
dc.date.available.fl_str_mv	2017-12-12T12:41:16Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SILVA, M. M. C. da et al. Potential biomarkers for the clinical prognosis of severe dengue. Memorias Do Instituto Oswaldo Cruz, v. 108, n. 6, p. 755–762, set. 2013.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/23617
dc.identifier.issn.pt_BR.fl_str_mv	1678-8060
dc.identifier.doi.none.fl_str_mv	10.1590/0074-0276108062013012
identifier_str_mv	SILVA, M. M. C. da et al. Potential biomarkers for the clinical prognosis of severe dengue. Memorias Do Instituto Oswaldo Cruz, v. 108, n. 6, p. 755–762, set. 2013. 1678-8060 10.1590/0074-0276108062013012
url	https://www.arca.fiocruz.br/handle/icict/23617
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
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reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Potential biomarkers for the clinical prognosis of severe dengue

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