Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/5487 |
Resumo: | Acknowledgements The authors thank Dr. Lygia Paraense (FIOCRUZ, Rio de Janeiro, Brazil) for kindly providing infected mice and Jose´ Ferreira Oliveira for skilful technical assistance. FUJB (8992-3), FAPERJ and CNPq (Brazil) supported this work. F.N., F.O.P. and V.F.M.S. are recipients of CNPq and FAPERJ fellowships |
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Silva, C. L. M.Lenzi, Henrique LeonelSilva, V. F. M.Paulo, F. O.Noel, F.2012-09-06T14:37:51Z2012-09-06T14:37:51Z2003SILVA, C. L. M. ; LENZI, H. L. ; SILVA, V. F. et al. Cellular mechanisms involved in the increased contration of portal veins from Schistosoma mansoni infected mice. Parasitology Research, v. 89, n. 1, p. 16-22, 2003.0932-0113https://www.arca.fiocruz.br/handle/icict/5487DOI 10.1007/s00436-002-0711-7Acknowledgements The authors thank Dr. Lygia Paraense (FIOCRUZ, Rio de Janeiro, Brazil) for kindly providing infected mice and Jose´ Ferreira Oliveira for skilful technical assistance. FUJB (8992-3), FAPERJ and CNPq (Brazil) supported this work. F.N., F.O.P. and V.F.M.S. are recipients of CNPq and FAPERJ fellowshipsUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, BrasilWe previously reported that portal veins from mice infected with male Schistosoma mansoni exhibited an increased reactivity to 5-hydroxytryptamine (5-HT). Here, we extended our observations to mice infected by both male and female worms and we further investigated another constrictor agent and the mechanism(s) responsible for the enhanced maximal contraction ( E(max)). Bisexual infection increased the E(max) of 5-HT (from 0.66+/-0.06 mN.s to 1.56+/-0.38 mN.s), in a similar way to the unisexual (male) infection. Infection with male worms increased portal vein reactivity to acetylcholine, as revealed by a higher E(max) (1.03+/-0.2 mN.s) in relation to non-infected control animals ( E(max)= 0.54+/-0.08 mN.s). Sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA) inhibition with 100 nM thapsigargin reduced the E(max) of 5-HT by 35% in both tissues, discharging a deficiency of SERCA pump in infected animals. In contrast, the number of voltage-dependent Ca(2+) channels (L-type) was higher in portal veins from infected than non-infected control mice. Inhibition of Ca(2+)-activated chloride channels (Cl(Ca)) with 10 micro M niflumic acid reduced the E(max) of 5-HT in portal veins more from infected than non-infected animals (remaining tension = 60.9+/-2.2% and 70.4+/-2.3%, respectively). Histopathological analysis revealed an increased content of collagen and elastin in portal veins from male S. mansoni-infected mice, compatible with an increased intraluminal pressure. In conclusion, male S. mansoni altered portal vein physiology, increasing the E(max) of two vasoconstrictors, possibly by increasing membrane depolarisation through a more effective opening of Cl(Ca) channels, with calcium entering through L-type Ca(2+) channels.engSpringer Verlag (Germany)Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAcetylcholineDose-Response RelationshipSchistosoma mansonicalciumSerotoninSex FactorsSarcoplasmic ReticulumRetículo SarcoplasmáticoSchistosoma mansoniAcetilcolinaAntagonistas da SerotoninaCálcioFatores SexuaisHistocitoquímicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCellular mechanisms involved in the increased contraction.pdfCellular mechanisms involved in the increased contraction.pdfapplication/pdf341060https://www.arca.fiocruz.br/bitstream/icict/5487/1/Cellular%20mechanisms%20involved%20in%20the%20increased%20contraction.pdf78daff0433a46574f0760cc87c4fd9a4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5487/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTCellular mechanisms involved in the increased contraction.pdf.txtCellular mechanisms involved in the increased contraction.pdf.txtExtracted texttext/plain33504https://www.arca.fiocruz.br/bitstream/icict/5487/5/Cellular%20mechanisms%20involved%20in%20the%20increased%20contraction.pdf.txtaf6aecd5a23b1365316c32c037c08ca3MD55THUMBNAILCellular mechanisms involved in the increased contraction.pdf.jpgCellular mechanisms involved in the increased contraction.pdf.jpgGenerated Thumbnailimage/jpeg2304https://www.arca.fiocruz.br/bitstream/icict/5487/4/Cellular%20mechanisms%20involved%20in%20the%20increased%20contraction.pdf.jpg2dd5a257fe8ff1dc963f27739a88e21dMD54icict/54872018-04-06 09:06:44.847oai:www.arca.fiocruz.br:icict/5487Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:06:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| title |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| spellingShingle |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice Silva, C. L. M. Acetylcholine Dose-Response Relationship Schistosoma mansoni calcium Serotonin Sex Factors Sarcoplasmic Reticulum Retículo Sarcoplasmático Schistosoma mansoni Acetilcolina Antagonistas da Serotonina Cálcio Fatores Sexuais Histocitoquímica |
| title_short |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| title_full |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| title_fullStr |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| title_full_unstemmed |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| title_sort |
Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice |
| author |
Silva, C. L. M. |
| author_facet |
Silva, C. L. M. Lenzi, Henrique Leonel Silva, V. F. M. Paulo, F. O. Noel, F. |
| author_role |
author |
| author2 |
Lenzi, Henrique Leonel Silva, V. F. M. Paulo, F. O. Noel, F. |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Silva, C. L. M. Lenzi, Henrique Leonel Silva, V. F. M. Paulo, F. O. Noel, F. |
| dc.subject.en.pt_BR.fl_str_mv |
Acetylcholine Dose-Response Relationship Schistosoma mansoni calcium Serotonin Sex Factors Sarcoplasmic Reticulum |
| topic |
Acetylcholine Dose-Response Relationship Schistosoma mansoni calcium Serotonin Sex Factors Sarcoplasmic Reticulum Retículo Sarcoplasmático Schistosoma mansoni Acetilcolina Antagonistas da Serotonina Cálcio Fatores Sexuais Histocitoquímica |
| dc.subject.decs.pt_BR.fl_str_mv |
Retículo Sarcoplasmático Schistosoma mansoni Acetilcolina Antagonistas da Serotonina Cálcio Fatores Sexuais Histocitoquímica |
| description |
Acknowledgements The authors thank Dr. Lygia Paraense (FIOCRUZ, Rio de Janeiro, Brazil) for kindly providing infected mice and Jose´ Ferreira Oliveira for skilful technical assistance. FUJB (8992-3), FAPERJ and CNPq (Brazil) supported this work. F.N., F.O.P. and V.F.M.S. are recipients of CNPq and FAPERJ fellowships |
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2003 |
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2003 |
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2012-09-06T14:37:51Z |
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2012-09-06T14:37:51Z |
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SILVA, C. L. M. ; LENZI, H. L. ; SILVA, V. F. et al. Cellular mechanisms involved in the increased contration of portal veins from Schistosoma mansoni infected mice. Parasitology Research, v. 89, n. 1, p. 16-22, 2003. |
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https://www.arca.fiocruz.br/handle/icict/5487 |
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0932-0113 |
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DOI 10.1007/s00436-002-0711-7 |
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SILVA, C. L. M. ; LENZI, H. L. ; SILVA, V. F. et al. Cellular mechanisms involved in the increased contration of portal veins from Schistosoma mansoni infected mice. Parasitology Research, v. 89, n. 1, p. 16-22, 2003. 0932-0113 DOI 10.1007/s00436-002-0711-7 |
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