HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells

Detalhes bibliográficos
Autor(a) principal: Firmino, Alisson de Aquino
Data de Publicação: 2023
Outros Autores: Gomes Filho, Paulo Roberto Tavares, Martins, Adenilda Lima Lopes, Araújo, Thessika Hialla, Gois, Luana Leandro, Batista, Everton da Silva, Araújo, Jean Paulo Lacerda, Castro, Bernardo Galvão, Grassi, Maria Fernanda Rios
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58968
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Fundação Nacional para o Desenvolvimento do Ensino Superior Privado (FUNADESP). Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq).
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spelling Firmino, Alisson de AquinoGomes Filho, Paulo Roberto TavaresMartins, Adenilda Lima LopesAraújo, Thessika HiallaGois, Luana LeandroBatista, Everton da SilvaAraújo, Jean Paulo LacerdaCastro, Bernardo GalvãoGrassi, Maria Fernanda Rios2023-06-07T13:48:18Z2023-06-07T13:48:18Z2023FIRMINO, Alisson de Aquino et al. HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells. Pathogens, v. 12, p. 1-10, 2023.2076-0817https://www.arca.fiocruz.br/handle/icict/5896810.3390/pathogens12050682Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Fundação Nacional para o Desenvolvimento do Ensino Superior Privado (FUNADESP). Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq).Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Departamento de Saúde. Feira de Santana, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Escola Bahiana de Medicina e Saúde Pública. Centro Integrativo Multidisciplinar HTLV. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Background: The prevalence of human T-lymphotropic virus type-1 (HTLV-1) infection is higher in women, and sexual intercourse has been described as an important route of male-to-female transmission. The present study aimed to quantify HTLV-1 proviral load (PVL) in vaginal fluid, and to investigate correlations with PVL in peripheral blood mononuclear cells (PBMCs). In addition, cytopathological alterations and vaginal microbiota were evaluated. Methods: HTLV-1-infected women were consecutively recruited at a multidisciplinary center for HTLV patients in Salvador, Brazil. All women underwent gynecological examinations to obtain cervicovaginal fluid and venipuncture for blood collection. PVL, as measured by real-time quantitative polymerase chain reaction (RT–qPCR), was expressed as the number of copies of HTLV-1/106 cells in blood and vaginal fluid samples. Light microscopy was used to assess cervicovaginal cytopathology and vaginal microbiota. Results: In the 56 included women (43 asymptomatic carriers and 13 diagnosed with HTLV-1-associated myelopathy/tropical spastic paraparesis—HAM/TSP), mean age was 35.9 (SD ± 7.2) years. PVL was higher in PBMCs (median: 23,264 copies/106 cells; IQR: 6776–60,036) than in vaginal fluid (451.9 copies/106 cells; IQR: 0–2490) (p < 0.0001). PVL in PBMCs was observed to correlate directly with PVL in vaginal fluid (R = 0.37, p = 0.006). PVL was detected in the vaginal fluid of 24 of 43 (55.8%) asymptomatic women compared to 12 of 13 (92.3%) HAM/TSP patients, p = 0.02. Cytopathologic analyses revealed no differences between women with detectable or undetectable PVL. Conclusion: HTLV-1 proviral load is detectable in vaginal fluid and correlates directly with proviral load in peripheral blood. This finding suggests that sexual transmission of HTLV-1 from females to males may occur, as well as vertical transmission, particularly in the context of vaginal delivery.engMDPIHTLV-1Carga proviralCarga proviral vaginalHTLV-1Proviral loadVaginal proviral loadVírus Linfotrópico T Tipo 1 HumanoIntegração viralHTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58968/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDe Aquino, F. A. HTLV-1 Proviral Load in....pdfDe Aquino, F. A. HTLV-1 Proviral Load in....pdfapplication/pdf577739https://www.arca.fiocruz.br/bitstream/icict/58968/2/De%20Aquino%2c%20%20F.%20A.%20HTLV-1%20Proviral%20Load%20in....pdfd0f476443501f39dff2180b9eeb2ad3eMD52icict/589682023-06-07 10:48:20.297oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-07T13:48:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
title HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
spellingShingle HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
Firmino, Alisson de Aquino
HTLV-1
Carga proviral
Carga proviral vaginal
HTLV-1
Proviral load
Vaginal proviral load
Vírus Linfotrópico T Tipo 1 Humano
Integração viral
title_short HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
title_full HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
title_fullStr HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
title_full_unstemmed HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
title_sort HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells
author Firmino, Alisson de Aquino
author_facet Firmino, Alisson de Aquino
Gomes Filho, Paulo Roberto Tavares
Martins, Adenilda Lima Lopes
Araújo, Thessika Hialla
Gois, Luana Leandro
Batista, Everton da Silva
Araújo, Jean Paulo Lacerda
Castro, Bernardo Galvão
Grassi, Maria Fernanda Rios
author_role author
author2 Gomes Filho, Paulo Roberto Tavares
Martins, Adenilda Lima Lopes
Araújo, Thessika Hialla
Gois, Luana Leandro
Batista, Everton da Silva
Araújo, Jean Paulo Lacerda
Castro, Bernardo Galvão
Grassi, Maria Fernanda Rios
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Firmino, Alisson de Aquino
Gomes Filho, Paulo Roberto Tavares
Martins, Adenilda Lima Lopes
Araújo, Thessika Hialla
Gois, Luana Leandro
Batista, Everton da Silva
Araújo, Jean Paulo Lacerda
Castro, Bernardo Galvão
Grassi, Maria Fernanda Rios
dc.subject.other.en_US.fl_str_mv HTLV-1
Carga proviral
Carga proviral vaginal
topic HTLV-1
Carga proviral
Carga proviral vaginal
HTLV-1
Proviral load
Vaginal proviral load
Vírus Linfotrópico T Tipo 1 Humano
Integração viral
dc.subject.en.en_US.fl_str_mv HTLV-1
Proviral load
Vaginal proviral load
dc.subject.decs.en_US.fl_str_mv Vírus Linfotrópico T Tipo 1 Humano
Integração viral
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Fundação Nacional para o Desenvolvimento do Ensino Superior Privado (FUNADESP). Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq).
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-06-07T13:48:18Z
dc.date.available.fl_str_mv 2023-06-07T13:48:18Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIRMINO, Alisson de Aquino et al. HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells. Pathogens, v. 12, p. 1-10, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58968
dc.identifier.issn.en_US.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens12050682
identifier_str_mv FIRMINO, Alisson de Aquino et al. HTLV-1 proviral load in vaginal fluid correlates with levels in peripheral blood mononuclear cells. Pathogens, v. 12, p. 1-10, 2023.
2076-0817
10.3390/pathogens12050682
url https://www.arca.fiocruz.br/handle/icict/58968
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58968/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/58968/2/De%20Aquino%2c%20%20F.%20A.%20HTLV-1%20Proviral%20Load%20in....pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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