HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola

Detalhes bibliográficos
Autor(a) principal: Afonso, Joana Morais
Data de Publicação: 2012
Outros Autores: Bello, Gonzalo, Guimarães, Monick L., Sojka, Marta, Morgado, Mariza G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15806
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Agostinho Neto. Luanda, Angola.
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spelling Afonso, Joana MoraisBello, GonzaloGuimarães, Monick L.Sojka, MartaMorgado, Mariza G.2016-09-15T13:14:28Z2016-09-15T13:14:28Z2012AFONSO, JOana Morais; et al. HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola. Plos One, v.7, n.8, e42996, 11p, Aug. 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1580610.1371/journal.pone.0042996engPublic Library of ScienceAngolaEpidemia de HIV-1Diversidade genéticaResistência a medicamentosAngolaHIV-1 epidemicGenetic DiversityTransmitted Drug Resistance MutationsHIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angolainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Agostinho Neto. Luanda, Angola.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.São Lucas Medical Center. Kifangondo, Angola.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Background: Angola presents a very complex HIV-1 epidemic characterized by the co-circulation of several HIV-1 group M subtypes, intersubtype recombinants and unclassified (U) variants. The viral diversity outside the major metropolitan regions (Luanda and Cabinda) and the prevalence of transmitted drug resistance mutations (DRM) since the introduction of HAART in 2004, however, has been barely studied. Methods: One hundred and one individuals from the Central (n = 44), North (n = 35), and South (n = 22) regions of Angola were diagnosed as HIV-1 positive and had their blood collected between 2008 and 2010, at one of the National Referral Centers for HIV diagnosis, the Kifangondo Medical Center, located in the border between the Luanda and Bengo provinces. Angolan samples were genotyped based on phylogenetic and bootscanning analyses of the pol (PR/RT) gene and their drug resistance profile was analyzed. Results: Among the 101 samples analyzed, 51% clustered within a pure group M subtype, 42% were classified as intersubtype recombinants, and 7% were denoted as U. We observed an important variation in the prevalence of different HIV-1 genetic variants among country regions, with high frequency of subtype F1 in the North (20%), intersubtype recombinants in the Central (42%), and subtype C in the South (45%). Statistically significant difference in HIV-1 clade distribution was only observed in subtype C prevalence between North vs South (p = 0.0005) and Central vs South (p = 0.0012) regions. DRM to NRTI and/or NNRTI were detected in 16.3% of patients analyzed. Conclusions: These results demonstrate a heterogeneous distribution of HIV-1 genetic variants across different regions in Angola and also revealed an unexpected high frequency of DRM to RT inhibitors in patients that have reported no antiretroviral usage, which may decrease the efficiency of the standard first-line antiretroviral regimens currently used in the country.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15806/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoana_afonso_etal_IOC_2012.pdfjoana_afonso_etal_IOC_2012.pdfapplication/pdf1101642https://www.arca.fiocruz.br/bitstream/icict/15806/2/joana_afonso_etal_IOC_2012.pdf79d1e3ecb1c929b8e89ee619b6545ac8MD52TEXTjoana_afonso_etal_IOC_2012.pdf.txtjoana_afonso_etal_IOC_2012.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
title HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
spellingShingle HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
Afonso, Joana Morais
Angola
Epidemia de HIV-1
Diversidade genética
Resistência a medicamentos
Angola
HIV-1 epidemic
Genetic Diversity
Transmitted Drug Resistance Mutations
title_short HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
title_full HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
title_fullStr HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
title_full_unstemmed HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
title_sort HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola
author Afonso, Joana Morais
author_facet Afonso, Joana Morais
Bello, Gonzalo
Guimarães, Monick L.
Sojka, Marta
Morgado, Mariza G.
author_role author
author2 Bello, Gonzalo
Guimarães, Monick L.
Sojka, Marta
Morgado, Mariza G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Afonso, Joana Morais
Bello, Gonzalo
Guimarães, Monick L.
Sojka, Marta
Morgado, Mariza G.
dc.subject.other.pt_BR.fl_str_mv Angola
Epidemia de HIV-1
Diversidade genética
Resistência a medicamentos
topic Angola
Epidemia de HIV-1
Diversidade genética
Resistência a medicamentos
Angola
HIV-1 epidemic
Genetic Diversity
Transmitted Drug Resistance Mutations
dc.subject.en.pt_BR.fl_str_mv Angola
HIV-1 epidemic
Genetic Diversity
Transmitted Drug Resistance Mutations
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Agostinho Neto. Luanda, Angola.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-09-15T13:14:28Z
dc.date.available.fl_str_mv 2016-09-15T13:14:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AFONSO, JOana Morais; et al. HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola. Plos One, v.7, n.8, e42996, 11p, Aug. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15806
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0042996
identifier_str_mv AFONSO, JOana Morais; et al. HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola. Plos One, v.7, n.8, e42996, 11p, Aug. 2012.
1932-6203
10.1371/journal.pone.0042996
url https://www.arca.fiocruz.br/handle/icict/15806
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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