HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola

Detalhes bibliográficos
Autor(a) principal: Bártolo, Inês
Data de Publicação: 2014
Outros Autores: Zakovic, Susana, Martin, Francisco, Palladino, Claudia, Carvalho, Patrícia, Camacho, Ricardo, Thamm, Sven, Clemente, Sofia, Taveira, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/8231
Resumo: This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in AngolaHIV-1AngolaThis is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."Objectives: To assess HIV-1 diversity, transmission dynamics and prevalence of transmitted drug resistance (TDR) in Angola, five years after ART scale-up. Methods: Population sequencing of the pol gene was performed on 139 plasma samples collected in 2009 from drug-naive HIV-1 infected individuals living in Luanda. HIV-1 subtypes were determined using phylogenetic analysis. Drug resistance mutations were identified using the Calibrated Population Resistance Tool (CPR). Transmission networks were determined using phylogenetic analysis of all Angolan sequences present in the databases. Evolutionary trends were determined by comparison with a similar survey performed in 2001. Results: 47.1% of the viruses were pure subtypes (all except B), 47.1% were recombinants and 5.8% were untypable. The prevalence of subtype A decreased significantly from 2001 to 2009 (40.0% to 10.8%, P50.0019) while the prevalence of unique recombinant forms (URFs) increased.2-fold (40.0% to 83.1%, P,0.0001). The most frequent URFs comprised untypable sequences with subtypes H (U/H, n57, 10.8%), A (U/A, n56, 9.2%) and G (G/U, n54, 6.2%). Newly identified U/H recombinants formed a highly supported monophyletic cluster suggesting a local and common origin. TDR mutation K103N was found in one (0.7%) patient (1.6% in 2001). Out of the 364 sequences sampled for transmission network analysis, 130 (35.7%) were part of a transmission network. Forty eight transmission clusters were identified; the majority (56.3%) comprised sequences sampled in 2008–2010 in Luanda which is consistent with a locally fuelled epidemic. Very low genetic distance was found in 27 transmission pairs sampled in the same year, suggesting recent transmission events. Conclusions: Transmission of drug resistant strains was still negligible in Luanda in 2009, five years after the scale-up of ART. The dominance of small and recent transmission clusters and the emergence of new URFs are consistent with a rising HIV-1 epidemics mainly driven by heterosexual transmission."This work was supported by Fundação para a Ciência e Tecnologia (FCT) http://www.fct. pt), Portugal, (grants ‘‘PTDC/SAU-FAR/115290/ 2009’’ and ‘‘PTDC/SAU-EPI/122400/2010’’), and by Collaborative HIV and Anti-HIV Drug Resistance Network (CHAIN), from the European Union. Inês Bártolo was supported by a post-doc fellowship (SFRH/BPD/76225/2011) from Fundação para a Ciência e Tecnologia (FCT), Portugal. Suzana Zakovic was supported by a European Union studentship from the Erasmus Mundus Masters in Forensic Sciences (http://forensicerasmusmundus. blogs.lincoln.ac.uk/). Francisco Martin was supported by a PhD fellowship (SFRH/BD/87488/2012) from Fundação para a Ciência e Tecnologia (FCT), Portugal. Claudia Palladino was supported by a post-doc fellowship (SFRH/BPD/77448/2011) from Fundação para a Ciência e Tecnologia (FCT), Portugal. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.PLOSRepositório ComumBártolo, InêsZakovic, SusanaMartin, FranciscoPalladino, ClaudiaCarvalho, PatríciaCamacho, RicardoThamm, SvenClemente, SofiaTaveira, Nuno2015-04-08T14:10:44Z2014-12-052014-12-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/8231engBártolo I, Zakovic S, Martin F, Palladino C, Carvalho P, et al. (2014) HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola. PLoS ONE 9(12): e113626. doi:10.1371/journal.pone.01136261932-620310.1371/journal.pone.0113626info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T02:30:57Zoai:comum.rcaap.pt:10400.26/8231Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:16.097675Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
title HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
spellingShingle HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
Bártolo, Inês
HIV-1
Angola
title_short HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
title_full HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
title_fullStr HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
title_full_unstemmed HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
title_sort HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola
author Bártolo, Inês
author_facet Bártolo, Inês
Zakovic, Susana
Martin, Francisco
Palladino, Claudia
Carvalho, Patrícia
Camacho, Ricardo
Thamm, Sven
Clemente, Sofia
Taveira, Nuno
author_role author
author2 Zakovic, Susana
Martin, Francisco
Palladino, Claudia
Carvalho, Patrícia
Camacho, Ricardo
Thamm, Sven
Clemente, Sofia
Taveira, Nuno
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Bártolo, Inês
Zakovic, Susana
Martin, Francisco
Palladino, Claudia
Carvalho, Patrícia
Camacho, Ricardo
Thamm, Sven
Clemente, Sofia
Taveira, Nuno
dc.subject.por.fl_str_mv HIV-1
Angola
topic HIV-1
Angola
description This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-05
2014-12-05T00:00:00Z
2015-04-08T14:10:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/8231
url http://hdl.handle.net/10400.26/8231
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bártolo I, Zakovic S, Martin F, Palladino C, Carvalho P, et al. (2014) HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola. PLoS ONE 9(12): e113626. doi:10.1371/journal.pone.0113626
1932-6203
10.1371/journal.pone.0113626
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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