Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Tassia Cristina Bello de
Data de Publicação: 2016
Outros Autores: Doyen, Noelle, Cavaillon, Jean-Marc, Bruno, Sávio Freire, Campos, Monique Paiva de, Miranda, Luisa Helena Monteiro de, Madeira, Maria de Fátima, Belo, Vinícius Silva, Figueiredo, Fabiano Borges
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31203
Resumo: Universidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil.
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spelling Vasconcelos, Tassia Cristina Bello deDoyen, NoelleCavaillon, Jean-MarcBruno, Sávio FreireCampos, Monique Paiva deMiranda, Luisa Helena Monteiro deMadeira, Maria de FátimaBelo, Vinícius SilvaFigueiredo, Fabiano Borges2019-01-22T10:27:57Z2019-01-22T10:27:57Z2016VASCONCELOS, Tassia Cristina Bello de et al. Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features. Veterinary Parasitology, v. 227, p. 8-14, 2016.0304-4017https://www.arca.fiocruz.br/handle/icict/3120310.1016/j.vetpar.2016.07.0171873-2550engElsevierCytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological featuresinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil.Institut Pasteur. Unit Cytokines and Inflammation. Department of Infection & Epidemiology. Paris, France.Institut Pasteur. Unit Cytokines and Inflammation. Department of Infection & Epidemiology. Paris, France.Universidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidade Federal de São João del-Rei. Campus Centro-Oeste “Dona Lindu”. Divinópolis, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.In South America, visceral leishmaniasis is a zoonotic disease with severe evolution characteristics in humans, and dogs are its main reservoir. In this context, this study aimed to evaluate the clinical status of dogs from a Brazilian endemic area naturally, at Barra Mansa municipality, infected with Leishmania infantum, in conjunction with their histopathological profile and, in order to determine possible markers of susceptibility or resistance to the disease, parasitic DNA load, cytokine and iNOS mRNA expression profiles were investigated in lymph nodes. High levels of IFN-ɣ and IL-6 mRNA were detected. Both IFN-ɣ and IL-6 mRNA were associated with disorganization of the corticomedullary region. IFN-ɣ and TNF-α mRNA were associated with the absence of follicular hyperplasia. The regulatory pathway was remarkable with IL-10 mRNA detection and its significant association with the severity of the disease. Plasmacytosis and sinus histiocytosis were associated with high loads of parasitic DNA, but there was no significant association between the parasite DNA load and animal clinical alterations. Since high parasitic loads were found in animals with or without symptoms, clinical examination cannot be considered as a criterion for disease susceptibility assessment.Visceral leishmaniasisImmunopathologyCanine infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31203/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Vasconcelos_Tassia_etal_INI_2016.pdfve_Vasconcelos_Tassia_etal_INI_2016.pdfapplication/pdf1709103https://www.arca.fiocruz.br/bitstream/icict/31203/2/ve_Vasconcelos_Tassia_etal_INI_2016.pdf905265d15c4a86726a30a76d3b6a649fMD52TEXTve_Vasconcelos_Tassia_etal_INI_2016.pdf.txtve_Vasconcelos_Tassia_etal_INI_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
title Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
spellingShingle Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
Vasconcelos, Tassia Cristina Bello de
Visceral leishmaniasis
Immunopathology
Canine infection
title_short Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
title_full Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
title_fullStr Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
title_full_unstemmed Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
title_sort Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features
author Vasconcelos, Tassia Cristina Bello de
author_facet Vasconcelos, Tassia Cristina Bello de
Doyen, Noelle
Cavaillon, Jean-Marc
Bruno, Sávio Freire
Campos, Monique Paiva de
Miranda, Luisa Helena Monteiro de
Madeira, Maria de Fátima
Belo, Vinícius Silva
Figueiredo, Fabiano Borges
author_role author
author2 Doyen, Noelle
Cavaillon, Jean-Marc
Bruno, Sávio Freire
Campos, Monique Paiva de
Miranda, Luisa Helena Monteiro de
Madeira, Maria de Fátima
Belo, Vinícius Silva
Figueiredo, Fabiano Borges
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, Tassia Cristina Bello de
Doyen, Noelle
Cavaillon, Jean-Marc
Bruno, Sávio Freire
Campos, Monique Paiva de
Miranda, Luisa Helena Monteiro de
Madeira, Maria de Fátima
Belo, Vinícius Silva
Figueiredo, Fabiano Borges
dc.subject.en.pt_BR.fl_str_mv Visceral leishmaniasis
Immunopathology
Canine infection
topic Visceral leishmaniasis
Immunopathology
Canine infection
description Universidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-01-22T10:27:57Z
dc.date.available.fl_str_mv 2019-01-22T10:27:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VASCONCELOS, Tassia Cristina Bello de et al. Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features. Veterinary Parasitology, v. 227, p. 8-14, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31203
dc.identifier.issn.pt_BR.fl_str_mv 0304-4017
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetpar.2016.07.017
dc.identifier.eissn.none.fl_str_mv 1873-2550
identifier_str_mv VASCONCELOS, Tassia Cristina Bello de et al. Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features. Veterinary Parasitology, v. 227, p. 8-14, 2016.
0304-4017
10.1016/j.vetpar.2016.07.017
1873-2550
url https://www.arca.fiocruz.br/handle/icict/31203
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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