Ultrastructure of Zika virus particles in cell cultures

Detalhes bibliográficos
Autor(a) principal: Barreto-Vieira, Debora Ferreira
Data de Publicação: 2016
Outros Autores: Barth, Ortrud Monika, Silva, Marcos Alexandre Nunes da, Santos, Carolina Cardoso, Santos, Aline da Silva, F. Filho, Joaquim Batista, Filippis, Ana Maria Bispo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14604
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
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spelling Barreto-Vieira, Debora FerreiraBarth, Ortrud MonikaSilva, Marcos Alexandre Nunes daSantos, Carolina CardosoSantos, Aline da SilvaF. Filho, Joaquim BatistaFilippis, Ana Maria Bispo de2016-06-21T15:50:53Z2016-07-07T11:41:08Z2016-06-21T15:50:53Z2016-07-07T11:41:08Z2016BARRETO-VIEIRA, Debora; et al. Ultrastructure of Zika virus particles in cell cultures. Mem Inst Oswaldo Cruz, Mar. 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/1460410.1590/0074-027601601041678-8060engFundação Oswaldo CruzZika virusTécnicas de Cultura de CélulasZika virusVirus particlesCell cultureTransmission electron microcopyUltrastructure of Zika virus particles in cell culturesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública do Espírito Santo/Virologia. ES, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Since 2015 Zika virus (ZIKV) infected thousands of Brazilian people and spread to other American countries. Beside real time reverse transcriptase polimerase chain reaction (RT-PCR) diagnosis detecting the viral genome, virus isolation in cell lines was useful also and to study the viral particle structure and location inside the cells. This tool was achieved using transmission electron microscopy (TEM) of ZIKV infected cell lines. Human blood, obtained from a Brazilian patient during the first days of symptoms, was isolated in C6/36 mosquito cells and inoculated in sequence in Vero cells, fixed after six days, embedded in polymers, and ultrathin cut. This flavivirus, like dengue viruses, showed numerous virus particles after replication occurring inside vesicles of the endoplasmic reticulum confirming the susceptibility of the Vero cell line to ZIKV. TEM is the unique technique disposable to make the virus really visible.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTdebora_vieira_etal_IOC_2016.pdf.txtdebora_vieira_etal_IOC_2016.pdf.txtExtracted texttext/plain13900https://www.arca.fiocruz.br/bitstream/icict/14604/4/debora_vieira_etal_IOC_2016.pdf.txt6dff2d74a9840b82dad9b916b16441b1MD54ORIGINALdebora_vieira_etal_IOC_2016.pdfapplication/pdf797997https://www.arca.fiocruz.br/bitstream/icict/14604/2/debora_vieira_etal_IOC_2016.pdf76adca329a5af601c2326a34b50f9831MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14604/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146042022-06-24 12:59:50.335oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:59:50Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.pt_BR.fl_str_mv Ultrastructure of Zika virus particles in cell cultures
title Ultrastructure of Zika virus particles in cell cultures
spellingShingle Ultrastructure of Zika virus particles in cell cultures
Barreto-Vieira, Debora Ferreira
Zika virus
Técnicas de Cultura de Células
Zika virus
Virus particles
Cell culture
Transmission electron microcopy
title_short Ultrastructure of Zika virus particles in cell cultures
title_full Ultrastructure of Zika virus particles in cell cultures
title_fullStr Ultrastructure of Zika virus particles in cell cultures
title_full_unstemmed Ultrastructure of Zika virus particles in cell cultures
title_sort Ultrastructure of Zika virus particles in cell cultures
author Barreto-Vieira, Debora Ferreira
author_facet Barreto-Vieira, Debora Ferreira
Barth, Ortrud Monika
Silva, Marcos Alexandre Nunes da
Santos, Carolina Cardoso
Santos, Aline da Silva
F. Filho, Joaquim Batista
Filippis, Ana Maria Bispo de
author_role author
author2 Barth, Ortrud Monika
Silva, Marcos Alexandre Nunes da
Santos, Carolina Cardoso
Santos, Aline da Silva
F. Filho, Joaquim Batista
Filippis, Ana Maria Bispo de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barreto-Vieira, Debora Ferreira
Barth, Ortrud Monika
Silva, Marcos Alexandre Nunes da
Santos, Carolina Cardoso
Santos, Aline da Silva
F. Filho, Joaquim Batista
Filippis, Ana Maria Bispo de
dc.subject.other.pt_BR.fl_str_mv Zika virus
Técnicas de Cultura de Células
topic Zika virus
Técnicas de Cultura de Células
Zika virus
Virus particles
Cell culture
Transmission electron microcopy
dc.subject.en.pt_BR.fl_str_mv Zika virus
Virus particles
Cell culture
Transmission electron microcopy
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-21T15:50:53Z
2016-07-07T11:41:08Z
dc.date.available.fl_str_mv 2016-06-21T15:50:53Z
2016-07-07T11:41:08Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARRETO-VIEIRA, Debora; et al. Ultrastructure of Zika virus particles in cell cultures. Mem Inst Oswaldo Cruz, Mar. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14604
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-02760160104
dc.identifier.eissn.pt_BR.fl_str_mv 1678-8060
identifier_str_mv BARRETO-VIEIRA, Debora; et al. Ultrastructure of Zika virus particles in cell cultures. Mem Inst Oswaldo Cruz, Mar. 2016.
0074-0276
10.1590/0074-02760160104
1678-8060
url https://www.arca.fiocruz.br/handle/icict/14604
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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