Ultrastructure of Zika virus particles in cell cultures

Detalhes bibliográficos
Autor(a) principal: Barreto-Vieira,Debora Ferreira
Data de Publicação: 2016
Outros Autores: Barth,Ortrud Monika, Silva,Marcos Alexandre Nunes da, Santos,Carolina Cardoso, Santos,Aline da Silva, F Filho,Joaquim Batista, Filippis,Ana Maria Bispo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000800532
Resumo: Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.
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spelling Ultrastructure of Zika virus particles in cell culturesZika virusvirus particlescell culturetransmission electron microscopyZika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.Instituto Oswaldo Cruz, Ministério da Saúde2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000800532Memórias do Instituto Oswaldo Cruz v.111 n.8 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760160104info:eu-repo/semantics/openAccessBarreto-Vieira,Debora FerreiraBarth,Ortrud MonikaSilva,Marcos Alexandre Nunes daSantos,Carolina CardosoSantos,Aline da SilvaF Filho,Joaquim BatistaFilippis,Ana Maria Bispo deeng2020-04-25T17:52:27Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:19.818Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Ultrastructure of Zika virus particles in cell cultures
title Ultrastructure of Zika virus particles in cell cultures
spellingShingle Ultrastructure of Zika virus particles in cell cultures
Barreto-Vieira,Debora Ferreira
Zika virus
virus particles
cell culture
transmission electron microscopy
title_short Ultrastructure of Zika virus particles in cell cultures
title_full Ultrastructure of Zika virus particles in cell cultures
title_fullStr Ultrastructure of Zika virus particles in cell cultures
title_full_unstemmed Ultrastructure of Zika virus particles in cell cultures
title_sort Ultrastructure of Zika virus particles in cell cultures
author Barreto-Vieira,Debora Ferreira
author_facet Barreto-Vieira,Debora Ferreira
Barth,Ortrud Monika
Silva,Marcos Alexandre Nunes da
Santos,Carolina Cardoso
Santos,Aline da Silva
F Filho,Joaquim Batista
Filippis,Ana Maria Bispo de
author_role author
author2 Barth,Ortrud Monika
Silva,Marcos Alexandre Nunes da
Santos,Carolina Cardoso
Santos,Aline da Silva
F Filho,Joaquim Batista
Filippis,Ana Maria Bispo de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barreto-Vieira,Debora Ferreira
Barth,Ortrud Monika
Silva,Marcos Alexandre Nunes da
Santos,Carolina Cardoso
Santos,Aline da Silva
F Filho,Joaquim Batista
Filippis,Ana Maria Bispo de
dc.subject.por.fl_str_mv Zika virus
virus particles
cell culture
transmission electron microscopy
topic Zika virus
virus particles
cell culture
transmission electron microscopy
dc.description.none.fl_txt_mv Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.
description Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000800532
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000800532
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760160104
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.111 n.8 2016
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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