Binding of extracellular matrix proteins to Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Giannini, Maria José Soares Mendes
Data de Publicação: 2006
Outros Autores: Andreotti, Patrícia Ferrari, Vincenzi, Luciana Raquel, Silva, Juliana Leal Monteiro da, Lenzi, Henrique Leonel, Benard, Gil, Oliveira, Roseli Zancopé, Guedes, Herbert Leonel de Matos, Soares, Christiane Pienna
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5967
http://dx.doi.org/10.1016/j.micinf.2006.01.012
Resumo: Acknowledgements This investigation was financially supported by the Brazilian organizations, FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and PADC-FCF-UNESP.
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spelling Giannini, Maria José Soares MendesAndreotti, Patrícia FerrariVincenzi, Luciana RaquelSilva, Juliana Leal Monteiro daLenzi, Henrique LeonelBenard, GilOliveira, Roseli ZancopéGuedes, Herbert Leonel de MatosSoares, Christiane Pienna2012-12-12T16:01:18Z2012-12-12T16:01:18Z2006ANDREOTTI, P. F. et. al. Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes and Infection, v. 8, p. 1550-1559, 2006.1286-4579https://www.arca.fiocruz.br/handle/icict/5967http://dx.doi.org/10.1016/j.micinf.2006.01.012Acknowledgements This investigation was financially supported by the Brazilian organizations, FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and PADC-FCF-UNESP.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Alergia e Imunologia Clínica e Experimental. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara, São Paulo, Brasil.Adhesion to extracellular matrix (ECM) proteins plays a crucial role in invasive fungal diseases. ECM proteins bind to the surface of Paracoccidioides brasiliensis yeast cells in distinct qualitative patterns. Extracts from Pb18 strain, before (18a) and after animal inoculation (18b), exhibited differential adhesion to ECM components. Pb18b extract had a higher capacity for binding to ECM components than Pb18a. Laminin was the most adherent component for both samples, followed by type I collagen, fibronectin, and type IV collagen for Pb18b. A remarkable difference was seen in the interaction of the two extracts with fibronectin and their fragments. Pb18b extract interacted significantly with the 120-kDa fragment. Ligand affinity binding assays showed that type I collagen recognized two components (47 and 80 kDa) and gp43 bound both fibronectin and laminin. The peptide 1 (NLGRDAKRHL) from gp43, with several positively charged amino acids, contributed most to the adhesion of P. brasiliensis to Vero cells. Synthetic peptides derived from peptide YIGRS of laminin or from RGD of both laminin and fibronectin showed the greatest inhibition of adhesion of gp43 to Vero cells. In conclusion, this work provided new molecular details on the interaction between P. brasiliensis and ECM components.engElsevierClínica médicaDermatopatias infecciosasParacoccidioidomicoseMatriz extracelularCélulas epiteliaisParacoccidioides brasiliensisExtracellular matrixAdhesinsgp43Células epiteliaisMedicina ClínicaDermatopatias InfecciosasParacoccidioidomicoseMatriz ExtracelularAdesinas BacterianasBinding of extracellular matrix proteins to Paracoccidioides brasiliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81949https://www.arca.fiocruz.br/bitstream/icict/5967/2/license.txtaad6e5ff39a8b3723a1981fb3bcd1becMD52TEXTBinding of extracellular matrix proteins to Paracoccidioides brasiliensis.htm.txtBinding of extracellular matrix proteins to Paracoccidioides brasiliensis.htm.txtExtracted texttext/plain50271https://www.arca.fiocruz.br/bitstream/icict/5967/3/Binding%20of%20extracellular%20matrix%20proteins%20to%20Paracoccidioides%20brasiliensis.htm.txt6e80b69062f0511053f753a325dc1d64MD53Binding of extracellular matrix proteins.pdf.txtBinding of extracellular matrix proteins.pdf.txtExtracted texttext/plain46872https://www.arca.fiocruz.br/bitstream/icict/5967/7/Binding%20of%20extracellular%20matrix%20proteins.pdf.txtc1a8a4524771ad655c72dea99559ccafMD57ORIGINALBinding of extracellular matrix proteins.pdfBinding of extracellular matrix proteins.pdfapplication/pdf870830https://www.arca.fiocruz.br/bitstream/icict/5967/4/Binding%20of%20extracellular%20matrix%20proteins.pdf172dcfbee041071699f78f4fa466358bMD54THUMBNAILBinding of extracellular matrix proteins.pdf.jpgBinding of extracellular matrix proteins.pdf.jpgGenerated Thumbnailimage/jpeg2166https://www.arca.fiocruz.br/bitstream/icict/5967/6/Binding%20of%20extracellular%20matrix%20proteins.pdf.jpg43f1a29956dc0fb413169019b34a2d2fMD56icict/59672018-04-06 09:02:53.732oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:02:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
title Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
spellingShingle Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
Giannini, Maria José Soares Mendes
Clínica médica
Dermatopatias infecciosas
Paracoccidioidomicose
Matriz extracelular
Células epiteliais
Paracoccidioides brasiliensis
Extracellular matrix
Adhesins
gp43
Células epiteliais
Medicina Clínica
Dermatopatias Infecciosas
Paracoccidioidomicose
Matriz Extracelular
Adesinas Bacterianas
title_short Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
title_full Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
title_fullStr Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
title_full_unstemmed Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
title_sort Binding of extracellular matrix proteins to Paracoccidioides brasiliensis
author Giannini, Maria José Soares Mendes
author_facet Giannini, Maria José Soares Mendes
Andreotti, Patrícia Ferrari
Vincenzi, Luciana Raquel
Silva, Juliana Leal Monteiro da
Lenzi, Henrique Leonel
Benard, Gil
Oliveira, Roseli Zancopé
Guedes, Herbert Leonel de Matos
Soares, Christiane Pienna
author_role author
author2 Andreotti, Patrícia Ferrari
Vincenzi, Luciana Raquel
Silva, Juliana Leal Monteiro da
Lenzi, Henrique Leonel
Benard, Gil
Oliveira, Roseli Zancopé
Guedes, Herbert Leonel de Matos
Soares, Christiane Pienna
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Giannini, Maria José Soares Mendes
Andreotti, Patrícia Ferrari
Vincenzi, Luciana Raquel
Silva, Juliana Leal Monteiro da
Lenzi, Henrique Leonel
Benard, Gil
Oliveira, Roseli Zancopé
Guedes, Herbert Leonel de Matos
Soares, Christiane Pienna
dc.subject.other.pt_BR.fl_str_mv Clínica médica
Dermatopatias infecciosas
Paracoccidioidomicose
Matriz extracelular
Células epiteliais
topic Clínica médica
Dermatopatias infecciosas
Paracoccidioidomicose
Matriz extracelular
Células epiteliais
Paracoccidioides brasiliensis
Extracellular matrix
Adhesins
gp43
Células epiteliais
Medicina Clínica
Dermatopatias Infecciosas
Paracoccidioidomicose
Matriz Extracelular
Adesinas Bacterianas
dc.subject.en.pt_BR.fl_str_mv Paracoccidioides brasiliensis
Extracellular matrix
Adhesins
gp43
Células epiteliais
dc.subject.decs.pt_BR.fl_str_mv Medicina Clínica
Dermatopatias Infecciosas
Paracoccidioidomicose
Matriz Extracelular
Adesinas Bacterianas
description Acknowledgements This investigation was financially supported by the Brazilian organizations, FAPESP, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and PADC-FCF-UNESP.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2012-12-12T16:01:18Z
dc.date.available.fl_str_mv 2012-12-12T16:01:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ANDREOTTI, P. F. et. al. Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes and Infection, v. 8, p. 1550-1559, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5967
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1016/j.micinf.2006.01.012
identifier_str_mv ANDREOTTI, P. F. et. al. Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes and Infection, v. 8, p. 1550-1559, 2006.
1286-4579
url https://www.arca.fiocruz.br/handle/icict/5967
http://dx.doi.org/10.1016/j.micinf.2006.01.012
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Elsevier
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