Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum

Detalhes bibliográficos
Autor(a) principal: Ferreira, Ana G. C. Neves
Data de Publicação: 2002
Outros Autores: Perales, Jonas, Fox, Jay W., Shannon, John D., Makino, Débora L., Garratt, Richard C., Domont, Gilberto B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41062
Resumo: Publisher's version/PDF may be used after a 12 months embargo period. Acesso aberto em 01/04/2020 - https://www.jbc.org/content/277/15/13129.full.pdf
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spelling Ferreira, Ana G. C. NevesPerales, JonasFox, Jay W.Shannon, John D.Makino, Débora L.Garratt, Richard C.Domont, Gilberto B.2020-05-01T18:49:39Z2020-05-01T18:49:39Z2002FERREIRA, Ana G. C. Neves et al. Structural and Functional Analyses of DM43, a Snake Venom Metalloproteinase Inhibitor from Didelphis marsupialis Serum. The Journal of Biological Chemistry, v. 277, n. 15, p. 13129-13137, 2002.0021-9258https://www.arca.fiocruz.br/handle/icict/4106210.1074/jbc.M2005892001083-351XPublisher's version/PDF may be used after a 12 months embargo period. Acesso aberto em 01/04/2020 - https://www.jbc.org/content/277/15/13129.full.pdfFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.University of Virginia. Department of Microbiology. Charlottesville, Virginia, USA.University of Virginia. Department of Microbiology. Charlottesville, Virginia, USA.Universidade de São Paulo. Instituto de Física de Sâo Carlos. Departamento de Física e Informática. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de Sâo Carlos. Departamento de Física e Informática. São Carlos, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.DM43, an opossum serum protein inhibitor of snake venom metalloproteinases, has been completely sequenced, and its disulfide bond pattern has been experimentally determined. It shows homology to human alpha(1)B-glycoprotein, a plasma protein of unknown function and a member of the immunoglobulin supergene family. Size exclusion and dynamic laser light scattering data indicated that two monomers of DM43, each composed of three immunoglobulin-like domains, associated to form a homodimer in solution. Analysis of its glycan moiety showed the presence of N-acetylglucosamine, mannose, galactose, and sialic acid, most probably forming four biantennary N-linked chains. DM43 inhibited the fibrinogenolytic activities of bothrolysin and jararhagin and formed 1:1 stoichiometric stable complexes with both metalloproteinases. DM43 was ineffective against atrolysin C or A. No complex formation was detected between DM43 and jararhagin C, indicating the essential role of the metalloproteinase domain for interaction. Homology modeling based on the crystal structure of a killer cell inhibitory receptor suggested the existence of an I-type Ig fold, a hydrophobic dimerization surface and six surface loops potentially forming the metalloproteinase-binding surface on DM43.engAmerican Society for Biochemistry and Molecular BiologyAnálises Estruturais e FuncionaisDM43Veneno de CobraInibidor de metaloproteinaseSoro de Didelphis marsupialisStructural and Functional AnalysesDM43Snake VenomMetalloproteinase InhibitorDidelphis marsupialis SerumStructural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis seruminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
title Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
spellingShingle Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
Ferreira, Ana G. C. Neves
Análises Estruturais e Funcionais
DM43
Veneno de Cobra
Inibidor de metaloproteinase
Soro de Didelphis marsupialis
Structural and Functional Analyses
DM43
Snake Venom
Metalloproteinase Inhibitor
Didelphis marsupialis Serum
title_short Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
title_full Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
title_fullStr Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
title_full_unstemmed Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
title_sort Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum
author Ferreira, Ana G. C. Neves
author_facet Ferreira, Ana G. C. Neves
Perales, Jonas
Fox, Jay W.
Shannon, John D.
Makino, Débora L.
Garratt, Richard C.
Domont, Gilberto B.
author_role author
author2 Perales, Jonas
Fox, Jay W.
Shannon, John D.
Makino, Débora L.
Garratt, Richard C.
Domont, Gilberto B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Ana G. C. Neves
Perales, Jonas
Fox, Jay W.
Shannon, John D.
Makino, Débora L.
Garratt, Richard C.
Domont, Gilberto B.
dc.subject.other.pt_BR.fl_str_mv Análises Estruturais e Funcionais
DM43
Veneno de Cobra
Inibidor de metaloproteinase
Soro de Didelphis marsupialis
topic Análises Estruturais e Funcionais
DM43
Veneno de Cobra
Inibidor de metaloproteinase
Soro de Didelphis marsupialis
Structural and Functional Analyses
DM43
Snake Venom
Metalloproteinase Inhibitor
Didelphis marsupialis Serum
dc.subject.en.pt_BR.fl_str_mv Structural and Functional Analyses
DM43
Snake Venom
Metalloproteinase Inhibitor
Didelphis marsupialis Serum
description Publisher's version/PDF may be used after a 12 months embargo period. Acesso aberto em 01/04/2020 - https://www.jbc.org/content/277/15/13129.full.pdf
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-05-01T18:49:39Z
dc.date.available.fl_str_mv 2020-05-01T18:49:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Ana G. C. Neves et al. Structural and Functional Analyses of DM43, a Snake Venom Metalloproteinase Inhibitor from Didelphis marsupialis Serum. The Journal of Biological Chemistry, v. 277, n. 15, p. 13129-13137, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41062
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.doi.none.fl_str_mv 10.1074/jbc.M200589200
dc.identifier.eissn.none.fl_str_mv 1083-351X
identifier_str_mv FERREIRA, Ana G. C. Neves et al. Structural and Functional Analyses of DM43, a Snake Venom Metalloproteinase Inhibitor from Didelphis marsupialis Serum. The Journal of Biological Chemistry, v. 277, n. 15, p. 13129-13137, 2002.
0021-9258
10.1074/jbc.M200589200
1083-351X
url https://www.arca.fiocruz.br/handle/icict/41062
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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