Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.

Detalhes bibliográficos
Autor(a) principal: Talvani, André
Data de Publicação: 2003
Outros Autores: Santana, Gilcinea, Barcelos, Lucíola da Silva, Ishii, Satoshi, Shimizu, Takao, Romanha, Álvaro José, Silva, João Santana da, Soares, Milena Botelho Pereira, Teixeira, Mauro Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9922
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
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spelling Talvani, AndréSantana, GilcineaBarcelos, Lucíola da SilvaIshii, SatoshiShimizu, TakaoRomanha, Álvaro JoséSilva, João Santana daSoares, Milena Botelho PereiraTeixeira, Mauro Martins2015-04-08T14:15:28Z2015-04-08T14:15:28Z2003TALVANI, A. et al. Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice. Microbes and Infection, v. 5, n. 9, p. 789-796, 2003.1286-4579https://www.arca.fiocruz.br/handle/icict/992210.1016/S1286-4579(03)00146-1engElsevierExperimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversity of Tokyo. Faculty of Medicine. Department of Biochemistry and Molecular Biology. Tokyo, Japan / CREST of Japan Science and Technology Corporation. Tokyo, JapanUniversity of Tokyo. Faculty of Medicine. Department of Biochemistry and Molecular Biology. Tokyo, Japan / CREST of Japan Science and Technology Corporation. Tokyo, JapanFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilThe generation of an inflammatory response driven by Trypanosoma cruzi or its subproducts appears to be essential for tissue injury and disease pathogenesis. However, this inflammatory response is also relevant in the control of T. cruzi replication. The lipid mediator platelet-activating factor (PAF) has been implicated in a number of pathological conditions characterized by tissue inflammation. In the present study, we aimed at evaluating the role of PAF during T. cruzi infection by using mice that were genetically deficient in the PAF receptor. We observed that infected hearts of PAFR(-/-) mice had an increased number of parasite nests, associated with a more intense inflammatory infiltrate. This was associated with greater parasitemia and lethality. When wild-type and PAFR(-/-) mice were compared, there were no marked changes in the kinetics of the expression of MCP-1, RANTES, IFN-gamma and TNF-alpha in heart tissue of infected animals. Moreover, serum concentrations of TNF-alpha, nitrate and parasite-specific IgM were similar in both groups of mice. In vitro, macrophages from PAFR(-/-) animals did not phagocytose trypomastigote forms when activated with PAF, leukotriene B(4) or MCP-1 and produced less nitric oxide when infected and activated with IFN-gamma. These results are consistent with the hypothesis that endogenous synthesis of PAF and activation of PAF receptors control T. cruzi replication in mice in great part via facilitation of the uptake of the parasite and consequent activation of macrophagesPlatelet-activating factorProtozoan infectionInflammationChemokinesTNF-aKnockoutCardiomiopatia Chagásica/imunologiaFator de Ativação de Plaquetas/fisiologiaGlicoproteínas da Membrana de Plaquetas/fisiologiaReceptores de Superfície Celular/fisiologiaTrypanosoma cruzi/crescimento & desenvolvimentoAnticorpos Antiprotozoários/sangueAnimaisCélulas CultivadasCardiomiopatia Chagásica/metabolismoCardiomiopatia Chagásica/parasitologiaQuimiocina CCL2/biossínteseFemininoCoração/parasitologiaImunoglobulina M/sangueInterferon gama/biossínteseLeucotrieno B4/farmacologiaMacrófagos/parasitologiaCamundongosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
title Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
spellingShingle Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
Talvani, André
Platelet-activating factor
Protozoan infection
Inflammation
Chemokines
TNF-a
Knockout
Cardiomiopatia Chagásica/imunologia
Fator de Ativação de Plaquetas/fisiologia
Glicoproteínas da Membrana de Plaquetas/fisiologia
Receptores de Superfície Celular/fisiologia
Trypanosoma cruzi/crescimento & desenvolvimento
Anticorpos Antiprotozoários/sangue
Animais
Células Cultivadas
Cardiomiopatia Chagásica/metabolismo
Cardiomiopatia Chagásica/parasitologia
Quimiocina CCL2/biossíntese
Feminino
Coração/parasitologia
Imunoglobulina M/sangue
Interferon gama/biossíntese
Leucotrieno B4/farmacologia
Macrófagos/parasitologia
Camundongos
title_short Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
title_full Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
title_fullStr Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
title_full_unstemmed Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
title_sort Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice.
author Talvani, André
author_facet Talvani, André
Santana, Gilcinea
Barcelos, Lucíola da Silva
Ishii, Satoshi
Shimizu, Takao
Romanha, Álvaro José
Silva, João Santana da
Soares, Milena Botelho Pereira
Teixeira, Mauro Martins
author_role author
author2 Santana, Gilcinea
Barcelos, Lucíola da Silva
Ishii, Satoshi
Shimizu, Takao
Romanha, Álvaro José
Silva, João Santana da
Soares, Milena Botelho Pereira
Teixeira, Mauro Martins
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Talvani, André
Santana, Gilcinea
Barcelos, Lucíola da Silva
Ishii, Satoshi
Shimizu, Takao
Romanha, Álvaro José
Silva, João Santana da
Soares, Milena Botelho Pereira
Teixeira, Mauro Martins
dc.subject.en.pt_BR.fl_str_mv Platelet-activating factor
Protozoan infection
Inflammation
Chemokines
TNF-a
Knockout
topic Platelet-activating factor
Protozoan infection
Inflammation
Chemokines
TNF-a
Knockout
Cardiomiopatia Chagásica/imunologia
Fator de Ativação de Plaquetas/fisiologia
Glicoproteínas da Membrana de Plaquetas/fisiologia
Receptores de Superfície Celular/fisiologia
Trypanosoma cruzi/crescimento & desenvolvimento
Anticorpos Antiprotozoários/sangue
Animais
Células Cultivadas
Cardiomiopatia Chagásica/metabolismo
Cardiomiopatia Chagásica/parasitologia
Quimiocina CCL2/biossíntese
Feminino
Coração/parasitologia
Imunoglobulina M/sangue
Interferon gama/biossíntese
Leucotrieno B4/farmacologia
Macrófagos/parasitologia
Camundongos
dc.subject.decs.pt_BR.fl_str_mv Cardiomiopatia Chagásica/imunologia
Fator de Ativação de Plaquetas/fisiologia
Glicoproteínas da Membrana de Plaquetas/fisiologia
Receptores de Superfície Celular/fisiologia
Trypanosoma cruzi/crescimento & desenvolvimento
Anticorpos Antiprotozoários/sangue
Animais
Células Cultivadas
Cardiomiopatia Chagásica/metabolismo
Cardiomiopatia Chagásica/parasitologia
Quimiocina CCL2/biossíntese
Feminino
Coração/parasitologia
Imunoglobulina M/sangue
Interferon gama/biossíntese
Leucotrieno B4/farmacologia
Macrófagos/parasitologia
Camundongos
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2015-04-08T14:15:28Z
dc.date.available.fl_str_mv 2015-04-08T14:15:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TALVANI, A. et al. Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice. Microbes and Infection, v. 5, n. 9, p. 789-796, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9922
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv 10.1016/S1286-4579(03)00146-1
identifier_str_mv TALVANI, A. et al. Experimental Trypanosoma cruzi infection in platelet-activating factor receptor-deficient mice. Microbes and Infection, v. 5, n. 9, p. 789-796, 2003.
1286-4579
10.1016/S1286-4579(03)00146-1
url https://www.arca.fiocruz.br/handle/icict/9922
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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