GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20446 |
Resumo: | Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil. |
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Lima, D. C.Duarte, Fábio T.Medeiros, Viviane Katielly SilvaCarvalho, Paulo CostaNogueira, Fábio Cesar SousaAraujo, Gabriel Duarte TavaresDumont, Gilberto BarbosaMedeiros, Sivia Regina Batistuzzo de2017-07-28T16:16:46Z2017-07-28T16:16:46Z2016LIMA, D. C. et al. GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. Scientific Reports, v. 6, n. 28174, p. 1-12, Jun. 16.2045-2322https://www.arca.fiocruz.br/handle/icict/2044610.1038/srep28174engNature Publishing GroupProteômicaChromobacteriumProteomicsClustered Regularly Interspaced Short Palindromic RepeatsHydrogen PeroxideChromobacteriumProteómicaRepeticiones Palindrómicas Cortas Agrupadas y Regularmente EspaciadasPeróxido de HidrógenoChromobacteriumRepetições Palindrômicas Curtas Agrupadas e Regularmente EspaçadasPeróxido de HidrogênioGeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil.Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte (IFRN). RN, Brasil.Universidade Federal do Vale do São Francisco. Colegiado de Medicina. Petrolina, PE, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Tecnologia. Laboratório de Química de Proteínas. Unidade Proteômica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil.Chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under different harsh environments such as oxidative and temperature stresses. Here we performed a labelfree quantitative proteomic study to unravel the molecular mechanisms that enable C. violaceum to survive oxidative stress. To achieve this, total proteins extracted from control and C. violaceum cultures exposed during two hours with 8mM hydrogen peroxide were analyzed using GeLC-MS proteomics. Analysis revealed that under the stress condition, the bacterium expressed proteins that protected it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins responsible for bacterial growth and catabolism. GeLC-MS proteomics analysis provided an overview of the metabolic pathways involved in the response of C. violaceum to oxidative stress ultimately aggregating knowledge of the response of this organism to environmental stress. This study identified approximately 1500 proteins, generating the largest proteomic coverage of C. violaceum so far. We also detected proteins with unknown function that we hypothesize to be part of new mechanisms related to oxidative stress defense. Finally, we identified the mechanism of clustered regularly interspaced short palindromic repeats (CRISPR), which has not yet been reported for this organism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/20446/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALGeLC-MS-based proteomics of.pdfGeLC-MS-based proteomics of.pdfapplication/pdf936344https://www.arca.fiocruz.br/bitstream/icict/20446/2/GeLC-MS-based%20proteomics%20of.pdfb9b5f5a3e8498a64bfb00b1d91d1fe12MD52TEXTGeLC-MS-based proteomics of.pdf.txtGeLC-MS-based proteomics of.pdf.txtExtracted texttext/plain60872https://www.arca.fiocruz.br/bitstream/icict/20446/3/GeLC-MS-based%20proteomics%20of.pdf.txtba7dcad2b87b3cfb7a232bea741b9d90MD53icict/204462019-11-01 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| dc.title.pt_BR.fl_str_mv |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| title |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| spellingShingle |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide Lima, D. C. Proteômica Chromobacterium Proteomics Clustered Regularly Interspaced Short Palindromic Repeats Hydrogen Peroxide Chromobacterium Proteómica Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas Peróxido de Hidrógeno Chromobacterium Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Peróxido de Hidrogênio |
| title_short |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| title_full |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| title_fullStr |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| title_full_unstemmed |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| title_sort |
GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide |
| author |
Lima, D. C. |
| author_facet |
Lima, D. C. Duarte, Fábio T. Medeiros, Viviane Katielly Silva Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Araujo, Gabriel Duarte Tavares Dumont, Gilberto Barbosa Medeiros, Sivia Regina Batistuzzo de |
| author_role |
author |
| author2 |
Duarte, Fábio T. Medeiros, Viviane Katielly Silva Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Araujo, Gabriel Duarte Tavares Dumont, Gilberto Barbosa Medeiros, Sivia Regina Batistuzzo de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, D. C. Duarte, Fábio T. Medeiros, Viviane Katielly Silva Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Araujo, Gabriel Duarte Tavares Dumont, Gilberto Barbosa Medeiros, Sivia Regina Batistuzzo de |
| dc.subject.other.pt_BR.fl_str_mv |
Proteômica |
| topic |
Proteômica Chromobacterium Proteomics Clustered Regularly Interspaced Short Palindromic Repeats Hydrogen Peroxide Chromobacterium Proteómica Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas Peróxido de Hidrógeno Chromobacterium Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Peróxido de Hidrogênio |
| dc.subject.en.pt_BR.fl_str_mv |
Chromobacterium Proteomics Clustered Regularly Interspaced Short Palindromic Repeats Hydrogen Peroxide |
| dc.subject.es.pt_BR.fl_str_mv |
Chromobacterium Proteómica Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas Peróxido de Hidrógeno |
| dc.subject.decs.pt_BR.fl_str_mv |
Chromobacterium Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Peróxido de Hidrogênio |
| description |
Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular e Genômica. Natal, RN, Brasil. |
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2016 |
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2016 |
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2017-07-28T16:16:46Z |
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2017-07-28T16:16:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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LIMA, D. C. et al. GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. Scientific Reports, v. 6, n. 28174, p. 1-12, Jun. 16. |
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https://www.arca.fiocruz.br/handle/icict/20446 |
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2045-2322 |
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10.1038/srep28174 |
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LIMA, D. C. et al. GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. Scientific Reports, v. 6, n. 28174, p. 1-12, Jun. 16. 2045-2322 10.1038/srep28174 |
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Nature Publishing Group |
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Nature Publishing Group |
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