Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Detalhes bibliográficos
Autor(a) principal: Machado, Guilherme S.
Data de Publicação: 2022
Outros Autores: Lopes, Rosane L., Oliveira, Simone S. C., Freitas, Simone P. C., Santos-Mallet, Jacenir R., Santos, André L. S., Feder, Denise, Gomes, Suzete A. O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52577
Resumo: Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.
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spelling Machado, Guilherme S.Lopes, Rosane L.Oliveira, Simone S. C.Freitas, Simone P. C.Santos-Mallet, Jacenir R.Santos, André L. S.Feder, DeniseGomes, Suzete A. O.2022-05-06T19:32:29Z2022-05-06T19:32:29Z2022MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022.2352-4146https://www.arca.fiocruz.br/handle/icict/5257710.4103/1995-7645.338437engScienceDirect.com by ElsevierTrypanosoma rangeliRhodnius prolixusFolículo ovarianoVitelogeninaProtease aspárticaCisteína proteaseTrypanosoma rangeliRhodnius prolixusOvarian follicleVitellogeninAspartic proteaseCysteine proteaseUltrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avanç ados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil.Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Nova Iguaçu, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avanç ados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies (sites of vitellogenesis) from Rhodnius (R.) prolixus infected with Trypanosoma (T.) rangeli. Methods: Adult virgin females of R. prolixus were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of T. rangeli epimastigotes (Macias strain). Females from the control group and all the males received parasitefree blood. Transmission electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected groups. Protein profile, proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected groups. Results: According to the ultrastructural data, T. rangeli infection elicited a degeneration process in the ovarian follicular cells of R. prolixus. Proteolytic assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group, while a significant increase in the cysteine peptidase activity was measured in both insect organs. Additionally, immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected insects. Conclusions: T. rangeli infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of R. prolixus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52577/3/license.txt5a560609d32a3863062d77ff32785d58MD53ORIGINALJacenirsantos_mallet_etal_IOC_2022.pdfJacenirsantos_mallet_etal_IOC_2022.pdfapplication/pdf1246671https://www.arca.fiocruz.br/bitstream/icict/52577/4/Jacenirsantos_mallet_etal_IOC_2022.pdf313f806ebd5c7d6a87902b9eae69475cMD54icict/525772022-05-06 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dc.title.pt_BR.fl_str_mv Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
title Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
spellingShingle Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
Machado, Guilherme S.
Trypanosoma rangeli
Rhodnius prolixus
Folículo ovariano
Vitelogenina
Protease aspártica
Cisteína protease
Trypanosoma rangeli
Rhodnius prolixus
Ovarian follicle
Vitellogenin
Aspartic protease
Cysteine protease
title_short Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
title_full Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
title_fullStr Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
title_full_unstemmed Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
title_sort Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
author Machado, Guilherme S.
author_facet Machado, Guilherme S.
Lopes, Rosane L.
Oliveira, Simone S. C.
Freitas, Simone P. C.
Santos-Mallet, Jacenir R.
Santos, André L. S.
Feder, Denise
Gomes, Suzete A. O.
author_role author
author2 Lopes, Rosane L.
Oliveira, Simone S. C.
Freitas, Simone P. C.
Santos-Mallet, Jacenir R.
Santos, André L. S.
Feder, Denise
Gomes, Suzete A. O.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Machado, Guilherme S.
Lopes, Rosane L.
Oliveira, Simone S. C.
Freitas, Simone P. C.
Santos-Mallet, Jacenir R.
Santos, André L. S.
Feder, Denise
Gomes, Suzete A. O.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma rangeli
Rhodnius prolixus
Folículo ovariano
Vitelogenina
Protease aspártica
Cisteína protease
topic Trypanosoma rangeli
Rhodnius prolixus
Folículo ovariano
Vitelogenina
Protease aspártica
Cisteína protease
Trypanosoma rangeli
Rhodnius prolixus
Ovarian follicle
Vitellogenin
Aspartic protease
Cysteine protease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma rangeli
Rhodnius prolixus
Ovarian follicle
Vitellogenin
Aspartic protease
Cysteine protease
description Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-05-06T19:32:29Z
dc.date.available.fl_str_mv 2022-05-06T19:32:29Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52577
dc.identifier.issn.pt_BR.fl_str_mv 2352-4146
dc.identifier.doi.none.fl_str_mv 10.4103/1995-7645.338437
identifier_str_mv MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022.
2352-4146
10.4103/1995-7645.338437
url https://www.arca.fiocruz.br/handle/icict/52577
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ScienceDirect.com by Elsevier
publisher.none.fl_str_mv ScienceDirect.com by Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52577/3/license.txt
https://www.arca.fiocruz.br/bitstream/icict/52577/4/Jacenirsantos_mallet_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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