Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59572 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. |
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Amaral, Lara CottaSalazar, Yanka Evellyn Alves RodriguesAlvarenga, Denise Anete Madureira dePina-Costa, Anielle deNunes, Ana Júlia DutraSouza Junior, Júlio Cesar deGonçalves, Gustavo Henrique PereiraBraga Hirano, Zelinda MariaMoreira, Sílvia BahadianPissinatti, AlcidesDaniel-Ribeiro, Cláudio TadeuSousa, Taís Nóbrega deBrito, Cristiana Ferreira Alves de2023-07-13T18:38:18Z2023-07-13T18:38:18Z2023AMARAL, Lara Cotta et al. Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest. Malaria journal, v. 22, n. 1, p. 1-11, 2023.1475-2875https://www.arca.fiocruz.br/handle/icict/5957210.1186/s12936-023-04601-7engBioMed CentralDetection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forestinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brazil / Universidade Federal Fluminense. Escola de Enfermagem Aurora de Afonso Costa. Departamento de Doenças Infecciosas e Parasitárias. Niterói, RJ, Brazil.Programa de Conservação do Bugio Ruivo. Joinville, SC, Brazil / Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brazil / Universidade Regional de Blumenau. Blumenau, SC, Brazil.Centro de Pesquisas Biológicas de Indaial, Indaial, SC, Brazil / Programa de Conservação do Bugio Ruivo. Joinville, SC, Brazil.Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brazil / Universidade Regional de Blumenau. Blumenau, SC, Brazil.Centro de Pesquisas Biológicas de Indaial. Indaial, SC, Brazil / Universidade Regional de Blumenau. Blumenau, SC, Brazil.Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brazil.Centro de Primatologia do Rio de Janeiro. Guapimirim, RJ, Brazil / Centro Universitário Serra dos Órgãos. Teresópolis, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil.Background: Plasmodium species of non-human primates (NHP) are of great interest because they can naturally infect humans. Plasmodium simium, a parasite restricted to the Brazilian Atlantic Forest, was recently shown to cause a zoonotic outbreak in the state of Rio de Janeiro. The potential of NHP to act as reservoirs of Plasmodium infection presents a challenge for malaria elimination, as NHP will contribute to the persistence of the parasite. The aim of the current study was to identify and quantify gametocytes in NHP naturally-infected by P. simium. Methods: Whole blood samples from 35 NHP were used in quantitative reverse transcription PCR (RT-qPCR) assays targeting 18S rRNA, Pss25 and Pss48/45 malaria parasite transcripts. Absolute quantification was performed in positive samples for 18S rRNA and Pss25 targets. Linear regression was used to compare the quantification cycle (Cq) and the Spearman's rank correlation coefficient was used to assess the correlation between the copy numbers of 18S rRNA and Pss25 transcripts. The number of gametocytes/µL was calculated by applying a conversion factor of 4.17 Pss25 transcript copies per gametocyte. Results: Overall, 87.5% of the 26 samples, previously diagnosed as P. simium, were positive for 18S rRNA transcript amplification, of which 13 samples (62%) were positive for Pss25 transcript amplification and 7 samples (54%) were also positive for Pss48/45 transcript. A strong positive correlation was identified between the Cq of the 18S rRNA and Pss25 and between the Pss25 and Pss48/45 transcripts. The 18S rRNA and Pss25 transcripts had an average of 1665.88 and 3.07 copies/µL, respectively. A positive correlation was observed between the copy number of Pss25 and 18S rRNA transcripts. Almost all gametocyte carriers exhibited low numbers of gametocytes (< 1/µL), with only one howler monkey having 5.8 gametocytes/µL. Conclusions: For the first time, a molecular detection of P. simium gametocytes in the blood of naturally-infected brown howler monkeys (Alouatta guariba clamitans) was reported here, providing evidence that they are likely to be infectious and transmit P. simium infection, and, therefore, may act as a reservoir of malaria infection for humans in the Brazilian Atlantic Forest.Plasmodium simiumPlasmodium species of non‑human primates (NHP)MalariaQuantify gametocytesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59572/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDetection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest - s12936-023-04601-7.pdfDetection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest - s12936-023-04601-7.pdfapplication/pdf292748https://www.arca.fiocruz.br/bitstream/icict/59572/2/Detection%20of%20Plasmodium%20simium%20gametocytes%20in%20non-human%20primates%20from%20the%20Brazilian%20Atlantic%20Forest%20-%20s12936-023-04601-7.pdf91eafc3ca4a95e719cb3c66d29ea9581MD52icict/595722023-09-04 11:10:22.019oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:10:22Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.en_US.fl_str_mv |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| title |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| spellingShingle |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest Amaral, Lara Cotta Plasmodium simium Plasmodium species of non‑human primates (NHP) Malaria Quantify gametocytes |
| title_short |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| title_full |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| title_fullStr |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| title_full_unstemmed |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| title_sort |
Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest |
| author |
Amaral, Lara Cotta |
| author_facet |
Amaral, Lara Cotta Salazar, Yanka Evellyn Alves Rodrigues Alvarenga, Denise Anete Madureira de Pina-Costa, Anielle de Nunes, Ana Júlia Dutra Souza Junior, Júlio Cesar de Gonçalves, Gustavo Henrique Pereira Braga Hirano, Zelinda Maria Moreira, Sílvia Bahadian Pissinatti, Alcides Daniel-Ribeiro, Cláudio Tadeu Sousa, Taís Nóbrega de Brito, Cristiana Ferreira Alves de |
| author_role |
author |
| author2 |
Salazar, Yanka Evellyn Alves Rodrigues Alvarenga, Denise Anete Madureira de Pina-Costa, Anielle de Nunes, Ana Júlia Dutra Souza Junior, Júlio Cesar de Gonçalves, Gustavo Henrique Pereira Braga Hirano, Zelinda Maria Moreira, Sílvia Bahadian Pissinatti, Alcides Daniel-Ribeiro, Cláudio Tadeu Sousa, Taís Nóbrega de Brito, Cristiana Ferreira Alves de |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amaral, Lara Cotta Salazar, Yanka Evellyn Alves Rodrigues Alvarenga, Denise Anete Madureira de Pina-Costa, Anielle de Nunes, Ana Júlia Dutra Souza Junior, Júlio Cesar de Gonçalves, Gustavo Henrique Pereira Braga Hirano, Zelinda Maria Moreira, Sílvia Bahadian Pissinatti, Alcides Daniel-Ribeiro, Cláudio Tadeu Sousa, Taís Nóbrega de Brito, Cristiana Ferreira Alves de |
| dc.subject.en.none.fl_str_mv |
Plasmodium simium Plasmodium species of non‑human primates (NHP) Malaria Quantify gametocytes |
| topic |
Plasmodium simium Plasmodium species of non‑human primates (NHP) Malaria Quantify gametocytes |
| description |
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. |
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2023 |
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2023-07-13T18:38:18Z |
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2023-07-13T18:38:18Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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AMARAL, Lara Cotta et al. Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest. Malaria journal, v. 22, n. 1, p. 1-11, 2023. |
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https://www.arca.fiocruz.br/handle/icict/59572 |
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1475-2875 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s12936-023-04601-7 |
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AMARAL, Lara Cotta et al. Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest. Malaria journal, v. 22, n. 1, p. 1-11, 2023. 1475-2875 10.1186/s12936-023-04601-7 |
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BioMed Central |
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