Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination

Detalhes bibliográficos
Autor(a) principal: Alencar, Bruna C. G. de
Data de Publicação: 2009
Outros Autores: Persechini, Pedro M., Haolla, Filipe A., Oliveira, Gabriel de, Silverio, Jaline C., Lannes-Vieira, Joseli, Machado, Alexandre V., Gazzinelli, Ricardo T., Bruna-Romero, Oscar, Rodrigues, Mauricio M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20807
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
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spelling Alencar, Bruna C. G. dePersechini, Pedro M.Haolla, Filipe A.Oliveira, Gabriel deSilverio, Jaline C.Lannes-Vieira, JoseliMachado, Alexandre V.Gazzinelli, Ricardo T.Bruna-Romero, OscarRodrigues, Mauricio M.2017-08-29T14:12:36Z2017-08-29T14:12:36Z2009ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009.0019-9567https://www.arca.fiocruz.br/handle/icict/2080710.1128/IAI.01459-081098-5522engAmerican Society for MicrobiologyTrypanosoma cruziPerforinaImunidadeTrypanosoma cruziPerforinCD8 T-CellimmunityinfectionPerforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccinationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Disease and Immunology. Worcester, Massachussets, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.A heterologous prime-boost strategy using plasmid DNA, followed by replication-defective recombinant adenovirus 5, is being proposed as a powerful way to elicit CD4(+) and CD8(+) T-cell-mediated protective immunity against intracellular pathogens. We confirmed this concept and furthered existing research by providing evidence that the heterologous prime-boost regimen using the gene encoding amastigote surface protein 2 elicited CD4(+) and CD8(+) T-cell-mediated protective immunity (reduction of acute parasitemia and prolonged survival) against experimental infection with Trypanosoma cruzi. Protective immunity correlated with the presence of in vivo antigen-specific cytotoxic activity prior to challenge. Based on this, our second goal was to determine the outcome of infection after heterologous prime-boost immunization of perforin-deficient mice. These mice were highly susceptible to infection. A detailed analysis of the cell-mediated immune responses in immunized perforin-deficient mice showed an impaired gamma interferon (IFN-gamma) secretion by immune spleen cells upon restimulation in vitro with soluble recombinant antigen. In spite of a normal numeric expansion, specific CD8(+) T cells presented several functional defects detected in vivo (cytotoxicity) and in vitro (simultaneous expression of CD107a/IFN-gamma or IFN-gamma/tumor necrosis factor alpha) paralleled by a decreased expression of CD44 and KLRG-1. Our final goal was to determine the importance of IFN-gamma in the presence of highly cytotoxic T cells. Vaccinated IFN-gamma-deficient mice developed highly cytotoxic cells but failed to develop any protective immunity. Our study thus demonstrated a role for perforin and IFN-gamma in a number of T-cell-mediated effector functions and in the antiparasitic immunity generated by a heterologous plasmid DNA prime-adenovirus boost vaccination strategy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20807/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoseli2_vieira_etal_IOC_2009.pdfjoseli2_vieira_etal_IOC_2009.pdfapplication/pdf2405002https://www.arca.fiocruz.br/bitstream/icict/20807/2/joseli2_vieira_etal_IOC_2009.pdfefc7e27c13811b5a4d6600d318adf67aMD52TEXTjoseli2_vieira_etal_IOC_2009.pdf.txtjoseli2_vieira_etal_IOC_2009.pdf.txtExtracted texttext/plain79852https://www.arca.fiocruz.br/bitstream/icict/20807/3/joseli2_vieira_etal_IOC_2009.pdf.txtcd81cd6bea3f89be38d2934bd734995dMD53icict/208072018-08-15 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dc.title.pt_BR.fl_str_mv Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
title Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
spellingShingle Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
Alencar, Bruna C. G. de
Trypanosoma cruzi
Perforina
Imunidade
Trypanosoma cruzi
Perforin
CD8 T-Cell
immunity
infection
title_short Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
title_full Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
title_fullStr Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
title_full_unstemmed Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
title_sort Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
author Alencar, Bruna C. G. de
author_facet Alencar, Bruna C. G. de
Persechini, Pedro M.
Haolla, Filipe A.
Oliveira, Gabriel de
Silverio, Jaline C.
Lannes-Vieira, Joseli
Machado, Alexandre V.
Gazzinelli, Ricardo T.
Bruna-Romero, Oscar
Rodrigues, Mauricio M.
author_role author
author2 Persechini, Pedro M.
Haolla, Filipe A.
Oliveira, Gabriel de
Silverio, Jaline C.
Lannes-Vieira, Joseli
Machado, Alexandre V.
Gazzinelli, Ricardo T.
Bruna-Romero, Oscar
Rodrigues, Mauricio M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alencar, Bruna C. G. de
Persechini, Pedro M.
Haolla, Filipe A.
Oliveira, Gabriel de
Silverio, Jaline C.
Lannes-Vieira, Joseli
Machado, Alexandre V.
Gazzinelli, Ricardo T.
Bruna-Romero, Oscar
Rodrigues, Mauricio M.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Perforina
Imunidade
topic Trypanosoma cruzi
Perforina
Imunidade
Trypanosoma cruzi
Perforin
CD8 T-Cell
immunity
infection
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Perforin
CD8 T-Cell
immunity
infection
description Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2017-08-29T14:12:36Z
dc.date.available.fl_str_mv 2017-08-29T14:12:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20807
dc.identifier.issn.pt_BR.fl_str_mv 0019-9567
dc.identifier.eissn.none.fl_str_mv 10.1128/IAI.01459-08
1098-5522
identifier_str_mv ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009.
0019-9567
10.1128/IAI.01459-08
1098-5522
url https://www.arca.fiocruz.br/handle/icict/20807
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
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