Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20807 |
Resumo: | Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. |
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Alencar, Bruna C. G. dePersechini, Pedro M.Haolla, Filipe A.Oliveira, Gabriel deSilverio, Jaline C.Lannes-Vieira, JoseliMachado, Alexandre V.Gazzinelli, Ricardo T.Bruna-Romero, OscarRodrigues, Mauricio M.2017-08-29T14:12:36Z2017-08-29T14:12:36Z2009ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009.0019-9567https://www.arca.fiocruz.br/handle/icict/2080710.1128/IAI.01459-081098-5522engAmerican Society for MicrobiologyTrypanosoma cruziPerforinaImunidadeTrypanosoma cruziPerforinCD8 T-CellimmunityinfectionPerforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccinationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular e Biologia das Interações. Rio de Janeiro, RJ. Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Disease and Immunology. Worcester, Massachussets, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.A heterologous prime-boost strategy using plasmid DNA, followed by replication-defective recombinant adenovirus 5, is being proposed as a powerful way to elicit CD4(+) and CD8(+) T-cell-mediated protective immunity against intracellular pathogens. We confirmed this concept and furthered existing research by providing evidence that the heterologous prime-boost regimen using the gene encoding amastigote surface protein 2 elicited CD4(+) and CD8(+) T-cell-mediated protective immunity (reduction of acute parasitemia and prolonged survival) against experimental infection with Trypanosoma cruzi. Protective immunity correlated with the presence of in vivo antigen-specific cytotoxic activity prior to challenge. Based on this, our second goal was to determine the outcome of infection after heterologous prime-boost immunization of perforin-deficient mice. These mice were highly susceptible to infection. A detailed analysis of the cell-mediated immune responses in immunized perforin-deficient mice showed an impaired gamma interferon (IFN-gamma) secretion by immune spleen cells upon restimulation in vitro with soluble recombinant antigen. In spite of a normal numeric expansion, specific CD8(+) T cells presented several functional defects detected in vivo (cytotoxicity) and in vitro (simultaneous expression of CD107a/IFN-gamma or IFN-gamma/tumor necrosis factor alpha) paralleled by a decreased expression of CD44 and KLRG-1. Our final goal was to determine the importance of IFN-gamma in the presence of highly cytotoxic T cells. Vaccinated IFN-gamma-deficient mice developed highly cytotoxic cells but failed to develop any protective immunity. Our study thus demonstrated a role for perforin and IFN-gamma in a number of T-cell-mediated effector functions and in the antiparasitic immunity generated by a heterologous plasmid DNA prime-adenovirus boost vaccination strategy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20807/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoseli2_vieira_etal_IOC_2009.pdfjoseli2_vieira_etal_IOC_2009.pdfapplication/pdf2405002https://www.arca.fiocruz.br/bitstream/icict/20807/2/joseli2_vieira_etal_IOC_2009.pdfefc7e27c13811b5a4d6600d318adf67aMD52TEXTjoseli2_vieira_etal_IOC_2009.pdf.txtjoseli2_vieira_etal_IOC_2009.pdf.txtExtracted texttext/plain79852https://www.arca.fiocruz.br/bitstream/icict/20807/3/joseli2_vieira_etal_IOC_2009.pdf.txtcd81cd6bea3f89be38d2934bd734995dMD53icict/208072018-08-15 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| dc.title.pt_BR.fl_str_mv |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| title |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| spellingShingle |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination Alencar, Bruna C. G. de Trypanosoma cruzi Perforina Imunidade Trypanosoma cruzi Perforin CD8 T-Cell immunity infection |
| title_short |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| title_full |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| title_fullStr |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| title_full_unstemmed |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| title_sort |
Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination |
| author |
Alencar, Bruna C. G. de |
| author_facet |
Alencar, Bruna C. G. de Persechini, Pedro M. Haolla, Filipe A. Oliveira, Gabriel de Silverio, Jaline C. Lannes-Vieira, Joseli Machado, Alexandre V. Gazzinelli, Ricardo T. Bruna-Romero, Oscar Rodrigues, Mauricio M. |
| author_role |
author |
| author2 |
Persechini, Pedro M. Haolla, Filipe A. Oliveira, Gabriel de Silverio, Jaline C. Lannes-Vieira, Joseli Machado, Alexandre V. Gazzinelli, Ricardo T. Bruna-Romero, Oscar Rodrigues, Mauricio M. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Alencar, Bruna C. G. de Persechini, Pedro M. Haolla, Filipe A. Oliveira, Gabriel de Silverio, Jaline C. Lannes-Vieira, Joseli Machado, Alexandre V. Gazzinelli, Ricardo T. Bruna-Romero, Oscar Rodrigues, Mauricio M. |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Perforina Imunidade |
| topic |
Trypanosoma cruzi Perforina Imunidade Trypanosoma cruzi Perforin CD8 T-Cell immunity infection |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Perforin CD8 T-Cell immunity infection |
| description |
Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. |
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2009 |
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2009 |
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2017-08-29T14:12:36Z |
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2017-08-29T14:12:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009. |
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https://www.arca.fiocruz.br/handle/icict/20807 |
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0019-9567 |
| dc.identifier.eissn.none.fl_str_mv |
10.1128/IAI.01459-08 1098-5522 |
| identifier_str_mv |
ALENCAR, Bianca C. G. de; et al. Perforin and Gamma Interferon Expression Are Required for CD4 and CD8 T-Cell-Dependent Protective Immunity against a Human Parasite, Trypanosoma cruzi, Elicited by Heterologous Plasmid DNA Prime-Recombinant Adenovirus 5 Boost Vaccination. Infection and Immunity, v.77, n.10, p.4383-4395, Oct. 2009. 0019-9567 10.1128/IAI.01459-08 1098-5522 |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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