The neovaginal microbiome of transgender women post-gender reassignment surgery

Detalhes bibliográficos
Autor(a) principal: Birse, Kenzie D.
Data de Publicação: 2020
Outros Autores: Kratzer, Kateryna, Zuend, Christina Farr, Mutch, Sarah, Noël-Romas, Laura, Lamont, Alana, Abou, Max, Jalil, Emilia, Veloso, Valdiléa G., Grinsztejn, Beatriz, Friedman, Ruth Khalili, Broliden, Kristina, Bradley, Frideborg, Poliquin, Vanessa, Li, Fan, Yanavich, Carolyn, Burgener, Adam, Aldrovandi, Grace
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41252
Resumo: Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.
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spelling Birse, Kenzie D.Kratzer, KaterynaZuend, Christina FarrMutch, SarahNoël-Romas, LauraLamont, AlanaAbou, MaxJalil, EmiliaVeloso, Valdiléa G.Grinsztejn, BeatrizFriedman, Ruth KhaliliBroliden, KristinaBradley, FrideborgPoliquin, VanessaLi, FanYanavich, CarolynBurgener, AdamAldrovandi, Grace2020-05-14T22:01:28Z2020-05-14T22:01:28Z2020BIRSE, Kenzie D. et al. The neovaginal microbiome of transgender women post-gender reassignment surgery. Microbiome, v. 8, n. 61, p. 1-13, 2020.2049-2618https://www.arca.fiocruz.br/handle/icict/4125210.1186/s40168-020-00804-1engBMCThe neovaginal microbiome of transgender women post-gender reassignment surgeryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePublic Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Karolinska University Hospital. Karolinska Institutet. Unit of Infectious Diseases. Center for Molecular Medicine. Department of Medicine Solna. Stockholm, Sweden.Karolinska University Hospital. Karolinska Institutet. Unit of Infectious Diseases. Center for Molecular Medicine. Department of Medicine Solna. Stockholm, Sweden.University of Manitoba. Department of Obstetrics & Gynecology. Winnipeg, MB, Canada.University of California. Los Angeles, CA, USA.University of California. Los Angeles, CA, USA.Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada / Karolinska University Hospital. Karolinska Institutet. Unit of Infectious Diseases. Center for Molecular Medicine. Department of Medicine Solna. Stockholm, Sweden / University of Manitoba. Department of Obstetrics & Gynecology. Winnipeg, MB, Canada.University of California. Los Angeles, CA, USA.Background: Gender reassignment surgery is a procedure some transgender women (TW) undergo for genderaffirming purposes. This often includes the construction of a neovagina using existing penile and scrotal tissue and/or a sigmoid colon graft. There are limited data regarding the composition and function of the neovaginal microbiome representing a major gap in knowledge in neovaginal health. Results: Metaproteomics was performed on secretions collected from the neovaginas (n = 5) and rectums (n = 7) of TW surgically reassigned via penile inversion/scrotal graft with (n = 1) or without (n = 4) a sigmoid colon graft extension and compared with secretions from cis vaginas (n = 32). We identified 541 unique bacterial proteins from 38 taxa. The most abundant taxa in the neovaginas were Porphyromonas (30.2%), Peptostreptococcus (9.2%), Prevotella (9.0%), Mobiluncus (8.0%), and Jonquetella (7.2%), while cis vaginas were primarily Lactobacillus and Gardnerella. Rectal samples were mainly composed of Prevotella and Roseburia. Neovaginas (median Shannon’s H index = 1.33) had higher alpha diversity compared to cis vaginas (Shannon’s H = 0.35) (p = 7.2E−3, Mann-Whitney U test) and were more similar to the non-Lactobacillus dominant/polymicrobial cis vaginas based on beta diversity (perMANOVA, p = 0.001, r 2 = 0.342). In comparison to cis vaginas, toll-like receptor response, amino acid, and shortchain fatty acid metabolic pathways were increased (p < 0.01), while keratinization and cornification proteins were decreased (p < 0.001) in the neovaginal proteome. Conclusions: Penile skin-lined neovaginas have diverse, polymicrobial communities that show similarities in composition to uncircumcised penises and host responses to cis vaginas with bacterial vaginosis (BV) including increased immune activation pathways and decreased epithelial barrier function. Developing a better understanding of microbiome-associated inflammation in the neovaginal environment will be important for improving our knowledge of neovaginal health.Gender reassignment surgeryMetaproteomicsMicrobiomeNeovaginaTransgender womeninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/41252/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALThe neovaginal_Emilia_Jalil_INI_LapClin-AIDS_2020.pdfThe neovaginal_Emilia_Jalil_INI_LapClin-AIDS_2020.pdfapplication/pdf1836874https://www.arca.fiocruz.br/bitstream/icict/41252/2/The%20neovaginal_Emilia_Jalil_INI_LapClin-AIDS_2020.pdf718632a7f9d347fa181d50854c6cdd73MD52TEXTThe neovaginal_Emilia_Jalil_INI_LapClin-AIDS_2020.pdf.txtThe neovaginal_Emilia_Jalil_INI_LapClin-AIDS_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv The neovaginal microbiome of transgender women post-gender reassignment surgery
title The neovaginal microbiome of transgender women post-gender reassignment surgery
spellingShingle The neovaginal microbiome of transgender women post-gender reassignment surgery
Birse, Kenzie D.
Gender reassignment surgery
Metaproteomics
Microbiome
Neovagina
Transgender women
title_short The neovaginal microbiome of transgender women post-gender reassignment surgery
title_full The neovaginal microbiome of transgender women post-gender reassignment surgery
title_fullStr The neovaginal microbiome of transgender women post-gender reassignment surgery
title_full_unstemmed The neovaginal microbiome of transgender women post-gender reassignment surgery
title_sort The neovaginal microbiome of transgender women post-gender reassignment surgery
author Birse, Kenzie D.
author_facet Birse, Kenzie D.
Kratzer, Kateryna
Zuend, Christina Farr
Mutch, Sarah
Noël-Romas, Laura
Lamont, Alana
Abou, Max
Jalil, Emilia
Veloso, Valdiléa G.
Grinsztejn, Beatriz
Friedman, Ruth Khalili
Broliden, Kristina
Bradley, Frideborg
Poliquin, Vanessa
Li, Fan
Yanavich, Carolyn
Burgener, Adam
Aldrovandi, Grace
author_role author
author2 Kratzer, Kateryna
Zuend, Christina Farr
Mutch, Sarah
Noël-Romas, Laura
Lamont, Alana
Abou, Max
Jalil, Emilia
Veloso, Valdiléa G.
Grinsztejn, Beatriz
Friedman, Ruth Khalili
Broliden, Kristina
Bradley, Frideborg
Poliquin, Vanessa
Li, Fan
Yanavich, Carolyn
Burgener, Adam
Aldrovandi, Grace
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Birse, Kenzie D.
Kratzer, Kateryna
Zuend, Christina Farr
Mutch, Sarah
Noël-Romas, Laura
Lamont, Alana
Abou, Max
Jalil, Emilia
Veloso, Valdiléa G.
Grinsztejn, Beatriz
Friedman, Ruth Khalili
Broliden, Kristina
Bradley, Frideborg
Poliquin, Vanessa
Li, Fan
Yanavich, Carolyn
Burgener, Adam
Aldrovandi, Grace
dc.subject.en.pt_BR.fl_str_mv Gender reassignment surgery
Metaproteomics
Microbiome
Neovagina
Transgender women
topic Gender reassignment surgery
Metaproteomics
Microbiome
Neovagina
Transgender women
description Public Health Agency of Canada. JC Wilt Infectious Disease Research Centre. National HIV and Retrovirology Labs. Winnipeg, MB, Canada / University of Manitoba. Departments of Medical Microbiology and Infectious Disease. Winnipeg, MB, Canada.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-05-14T22:01:28Z
dc.date.available.fl_str_mv 2020-05-14T22:01:28Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BIRSE, Kenzie D. et al. The neovaginal microbiome of transgender women post-gender reassignment surgery. Microbiome, v. 8, n. 61, p. 1-13, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41252
dc.identifier.issn.pt_BR.fl_str_mv 2049-2618
dc.identifier.doi.none.fl_str_mv 10.1186/s40168-020-00804-1
identifier_str_mv BIRSE, Kenzie D. et al. The neovaginal microbiome of transgender women post-gender reassignment surgery. Microbiome, v. 8, n. 61, p. 1-13, 2020.
2049-2618
10.1186/s40168-020-00804-1
url https://www.arca.fiocruz.br/handle/icict/41252
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dc.publisher.none.fl_str_mv BMC
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