Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C

Detalhes bibliográficos
Autor(a) principal: Almeida, R. W. de
Data de Publicação: 2015
Outros Autores: Espirito Santo, M. P., Sousa, P. S. F., Almeida, A. J. de, Lampe, E., Lewis-Ximenez, L. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13523
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
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spelling Almeida, R. W. deEspirito Santo, M. P.Sousa, P. S. F.Almeida, A. J. deLampe, E.Lewis-Ximenez, L. L.2016-04-05T11:00:44Z2016-04-05T11:00:44Z2015ALMEIDA, R. W. de; et al. Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C. Braz J Med Biol Res, v.48, n.6, p.553-556, June 2015.1414-431Xhttps://www.arca.fiocruz.br/handle/icict/1352310.1590/1414-431X20144040engAssociação Brasileira de Divulgação CientíficaHepatitis B virus DNA stability in plasma samples under short-term storage at 426Cinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 426C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/ mL. These were incubated at 426C for up to 7 days and then frozen at –706C. Viral load testing for HBV DNA was performed for all samples using COBAS1 AmpliPrep/COBAS1 TaqMan1 HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of this study demonstrated a decrease in HBV DNA viral load ranging from 0.005 to 0.30 log(10) IU/mL after storage at 426C for up to 7 days, these values did not exceed 0.5 log(10), which is the estimated intra-assay variation for molecular tests. Thus, the insignificant decrease in viral load suggests that shipment of HBV in plasma samples at temperatures of up to 426C is permissible if they are frozen within 7 days.HBVStability studiesReal-time PCRVírus da Hepatite BReação em Cadeia da Polimeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13523/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrw_almeida_etal_IOC_2015.pdfrw_almeida_etal_IOC_2015.pdfapplication/pdf150580https://www.arca.fiocruz.br/bitstream/icict/13523/2/rw_almeida_etal_IOC_2015.pdf84dc4734906345809e5654b84df9a6fbMD52TEXTrw_almeida_etal_IOC_2015.pdf.txtrw_almeida_etal_IOC_2015.pdf.txtExtracted texttext/plain17074https://www.arca.fiocruz.br/bitstream/icict/13523/3/rw_almeida_etal_IOC_2015.pdf.txt9e0e0f81d29c5d6df2a170f5bd20106dMD53icict/135232023-09-05 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dc.title.pt_BR.fl_str_mv Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
title Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
spellingShingle Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
Almeida, R. W. de
HBV
Stability studies
Real-time PCR
Vírus da Hepatite B
Reação em Cadeia da Polimerase
title_short Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
title_full Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
title_fullStr Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
title_full_unstemmed Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
title_sort Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C
author Almeida, R. W. de
author_facet Almeida, R. W. de
Espirito Santo, M. P.
Sousa, P. S. F.
Almeida, A. J. de
Lampe, E.
Lewis-Ximenez, L. L.
author_role author
author2 Espirito Santo, M. P.
Sousa, P. S. F.
Almeida, A. J. de
Lampe, E.
Lewis-Ximenez, L. L.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, R. W. de
Espirito Santo, M. P.
Sousa, P. S. F.
Almeida, A. J. de
Lampe, E.
Lewis-Ximenez, L. L.
dc.subject.en.pt_BR.fl_str_mv HBV
Stability studies
Real-time PCR
topic HBV
Stability studies
Real-time PCR
Vírus da Hepatite B
Reação em Cadeia da Polimerase
dc.subject.decs.pt_BR.fl_str_mv Vírus da Hepatite B
Reação em Cadeia da Polimerase
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-05T11:00:44Z
dc.date.available.fl_str_mv 2016-04-05T11:00:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, R. W. de; et al. Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C. Braz J Med Biol Res, v.48, n.6, p.553-556, June 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13523
dc.identifier.issn.none.fl_str_mv 1414-431X
dc.identifier.doi.none.fl_str_mv 10.1590/1414-431X20144040
identifier_str_mv ALMEIDA, R. W. de; et al. Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C. Braz J Med Biol Res, v.48, n.6, p.553-556, June 2015.
1414-431X
10.1590/1414-431X20144040
url https://www.arca.fiocruz.br/handle/icict/13523
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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