IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells

Detalhes bibliográficos
Autor(a) principal: Barbosa, Bellisa Freitas de
Data de Publicação: 2015
Outros Autores: Maria, Janice Buiate Lopes, Gomes, Angelica Oliveira, Angeloni, Mariana Bodini, Castro, Andressa Silva, Franco, Priscila Silva, Fermino, Marise Lopes, Barreira, Maria Cristina Roque, Ietta, Francesca, Martins Filho, Olindo Assis, Silva, Deise Aparecida Oliveira, Mineo, José Roberto, Ferro, Eloisa Amália Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14745
Resumo: Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.
id CRUZ_eb38dee4d29ae9ff1a8f440bcbbb0b36
oai_identifier_str oai:www.arca.fiocruz.br:icict/14745
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Barbosa, Bellisa Freitas deMaria, Janice Buiate LopesGomes, Angelica OliveiraAngeloni, Mariana BodiniCastro, Andressa SilvaFranco, Priscila SilvaFermino, Marise LopesBarreira, Maria Cristina RoqueIetta, FrancescaMartins Filho, Olindo AssisSilva, Deise Aparecida OliveiraMineo, José RobertoFerro, Eloisa Amália Vieira2016-07-01T18:43:55Z2016-07-07T11:36:19Z2016-07-01T18:43:55Z2016-07-07T11:36:19Z2015BARBOSA, Bellisa Freitas de et al. IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells. Biology of Reproduction, v. 92, n. 3, p. 1-13, 2015.1529-7268https://www.arca.fiocruz.br/handle/icict/1474510.1095/biolreprod.114.124115engSociety for the Study of ReproductionIL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.University of Siena. Department of Life Science. Siena, Italy.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.Considering that interleukin 10 (IL10), transforming growth factor beta1 (TGFB1), and interferon gamma (IFNG) are involved in the susceptibility of BeWo trophoblast cells to Toxoplasma gondii infection, the aim of the present study was to investigate the effector mechanisms triggered by these cytokines in the control of T. gondii in BeWo cells. For this purpose, infected/uninfected BeWo cells were treated with IL10, TGFB1 (50 ng/ml), and IFNG (20 or 100 ng/ml) in order to verify the phosphorylation of signal transducers and activators of transcription 1 (STAT1), STAT3, and Smad2, parasite intracellular proliferation, as well as the Th1/Th2/IL17A cytokine production. The treatment of BeWo cells with IL10 and TGFB1 favored T. gondii proliferation, and these findings were associated with STAT3 and Smad2 phosphorylation, respectively (P < 0.05). Also, these cytokine treatments were able to down-modulate TNF alpha (TNFA) and IL6 production (P < 0.05). Low concentration of IFNG was unable to control T. gondii infection but was able to trigger STAT1 phosphorylation and up-regulate IL6 and IL17A production; whereas a high concentration of IFNG was unable to activate STAT1 but down-modulated IL6 and TNFA and increased T. gondii proliferation (P < 0.05). IL10, TGFB1, and IFNG regulate a differential T. gondii proliferation in BeWo cells because they distinctly trigger intracellular signaling pathways and cytokine production, especially IL6 and TNFA. Our data open new windows to understand the mechanisms triggered by IL10, TGFB1, and IFNG at the maternal-fetal interface in the presence of T. gondii, contributing to recognizing the importance of these effector mechanisms involved in the vertical transmission of this parasite.CytokinesSignaling pathwaysToxoplasma gondiiTrophoblast cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTve_Belissa_Barbosa_IL10_CPqRR_2015.pdf.txtve_Belissa_Barbosa_IL10_CPqRR_2015.pdf.txtExtracted texttext/plain13https://www.arca.fiocruz.br/bitstream/icict/14745/4/ve_Belissa_Barbosa_IL10_CPqRR_2015.pdf.txt67e866c735744ec6037b77b623ee022dMD54ORIGINALve_Belissa_Barbosa_IL10_CPqRR_2015.pdfapplication/pdf5264566https://www.arca.fiocruz.br/bitstream/icict/14745/2/ve_Belissa_Barbosa_IL10_CPqRR_2015.pdfa10099780ceca849c3f52302a6a6471dMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14745/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/147452019-11-12 11:20:23.572oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-11-12T14:20:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
title IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
spellingShingle IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
Barbosa, Bellisa Freitas de
Cytokines
Signaling pathways
Toxoplasma gondii
Trophoblast cells
title_short IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
title_full IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
title_fullStr IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
title_full_unstemmed IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
title_sort IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells
author Barbosa, Bellisa Freitas de
author_facet Barbosa, Bellisa Freitas de
Maria, Janice Buiate Lopes
Gomes, Angelica Oliveira
Angeloni, Mariana Bodini
Castro, Andressa Silva
Franco, Priscila Silva
Fermino, Marise Lopes
Barreira, Maria Cristina Roque
Ietta, Francesca
Martins Filho, Olindo Assis
Silva, Deise Aparecida Oliveira
Mineo, José Roberto
Ferro, Eloisa Amália Vieira
author_role author
author2 Maria, Janice Buiate Lopes
Gomes, Angelica Oliveira
Angeloni, Mariana Bodini
Castro, Andressa Silva
Franco, Priscila Silva
Fermino, Marise Lopes
Barreira, Maria Cristina Roque
Ietta, Francesca
Martins Filho, Olindo Assis
Silva, Deise Aparecida Oliveira
Mineo, José Roberto
Ferro, Eloisa Amália Vieira
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Bellisa Freitas de
Maria, Janice Buiate Lopes
Gomes, Angelica Oliveira
Angeloni, Mariana Bodini
Castro, Andressa Silva
Franco, Priscila Silva
Fermino, Marise Lopes
Barreira, Maria Cristina Roque
Ietta, Francesca
Martins Filho, Olindo Assis
Silva, Deise Aparecida Oliveira
Mineo, José Roberto
Ferro, Eloisa Amália Vieira
dc.subject.en.pt_BR.fl_str_mv Cytokines
Signaling pathways
Toxoplasma gondii
Trophoblast cells
topic Cytokines
Signaling pathways
Toxoplasma gondii
Trophoblast cells
description Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-07-01T18:43:55Z
2016-07-07T11:36:19Z
dc.date.available.fl_str_mv 2016-07-01T18:43:55Z
2016-07-07T11:36:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Bellisa Freitas de et al. IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells. Biology of Reproduction, v. 92, n. 3, p. 1-13, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14745
dc.identifier.issn.pt_BR.fl_str_mv 1529-7268
dc.identifier.doi.pt_BR.fl_str_mv 10.1095/biolreprod.114.124115
identifier_str_mv BARBOSA, Bellisa Freitas de et al. IL10, TGF beta1, and IFN gamma modulate intracellular signaling pathways and cytokine production to control Toxoplasma gondii infection in BeWo trophoblast cells. Biology of Reproduction, v. 92, n. 3, p. 1-13, 2015.
1529-7268
10.1095/biolreprod.114.124115
url https://www.arca.fiocruz.br/handle/icict/14745
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/14745/4/ve_Belissa_Barbosa_IL10_CPqRR_2015.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/14745/2/ve_Belissa_Barbosa_IL10_CPqRR_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/14745/3/license.txt
bitstream.checksum.fl_str_mv 67e866c735744ec6037b77b623ee022d
a10099780ceca849c3f52302a6a6471d
5a560609d32a3863062d77ff32785d58
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324651714150400

Registros relacionados