Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response

Detalhes bibliográficos
Autor(a) principal: Pinna, Raquel A.
Data de Publicação: 2016
Outros Autores: Santos, Danielle Silva dos, Silva, Daiana S. Perce da, Ferreira, Joseli Oliveira, Verde, Dea M. S. Vila, Luca, Paula M. de, Banic, Dalma M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16556
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Simulídeos, Oncocercose e Infecções Simpáticas: Mansonerlose e Malária. Rio de Janeiro, RJ, Brasil.
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spelling Pinna, Raquel A.Santos, Danielle Silva dosSilva, Daiana S. Perce daFerreira, Joseli OliveiraVerde, Dea M. S. VilaLuca, Paula M. deBanic, Dalma M.2016-12-20T10:57:31Z2016-12-20T10:57:31Z2016PINNA, Raquel A. et al. Malaria - Cutaneous Leishmaniasis Co-infection: influence on disease outcomes and immune response. Frontiers in Microbiology, v.7, Article 982, 15p, June 2016.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/1655610.3389/fmicb.2016.00982engFrontier MediaMaláriaCo-infecçãoLeishmanioseImunologiaCitocinaCo-infectionMalariaLeishmaniasisimmunologycytokinePlasmodium yoeliiLeishmania brasiliensisLeishmania amazonensisMalaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Responseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Simulídeos, Oncocercose e Infecções Simpáticas: Mansonerlose e Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Simulídeos, Oncocercose e Infecções Simpáticas: Mansonerlose e Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Simulídeos, Oncocercose e Infecções Simpáticas: Mansonerlose e Malária. Rio de Janeiro, RJ, Brasil.Malaria and Cutaneous Leishmaniasis (CL) are co-endemic throughout large regions in tropical countries and co-infection may impact the evolution of host-parasite interactions. In the present study, we evaluate Malaria/Leishmaniasis disease outcome, Th1/Th2 cytokine levels and the CD4 and CD8 T-cell profiles in a co-infection murine model (BALB/c) of Plasmodium yoelii 17XNL (Py) and Leishmania amazonensis (La) or L. braziliensis (Lb). Malaria parasitaemia was assessed through blood strains stained with Giemsa. Leishmania lesions were monitored with a digital caliper and parasite loads determined by limiting-dilution assay. Serum levels of IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, and IL-17 were determined using multiplexed bead assay and expression of CD3, CD4, and CD8 T-cells markers were determined by Flow Cytometry in the thymus, spleens and lymph nodes. Parasitaemia in Lb+Py co-infected group was lower than in Py single-infected group, suggesting a protective effect of Lb co-infection in Malaria progression. In contrast, La+Py co-infection increased parasitaemia, patent infection and induced mortality in non-lethal Malaria infection. Regarding Leishmaniasis, Lb+Py co-infected group presented smaller lesions and less ulceration than Lb single-infected animals. In contrast, La+Py co-infected group presented only a transitory delay on the development of lesions when compared to La single-infected mice. Decreased levels of IFN-γ, TNF, IL-6, and IL-10 were observed in the serum of co-infected groups, demonstrating a modulation of Malaria immune response by Leishmania co-infections. We observed an intense thymic atrophy in Py single-infected and co-infected groups, which recovered earlier in co-infected animals. The CD4 and CD8 T cell profiles in thymus, spleens and lymph nodes did not differ between Py single and co-infected groups, except for a decrease in CD4(+)CD8(+) T cells which also increased faster in co-infected mice. Our results suggest that Py and Leishmania co-infection may change disease outcome. Interestingly Malaria outcome can be altered according to the Leishmania specie involved. Alternatively Malaria infection reduced the severity or delayed the onset of leishmanial lesions. These alterations in Malaria and CL development seem to be closely related with changes in the immune response as demonstrated by alteration in serum cytokine levels and thymus/spleens T cell phenotypes dynamics during infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16556/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALraquel_pinna_etal_IOC_2016.pdfraquel_pinna_etal_IOC_2016.pdfapplication/pdf2383252https://www.arca.fiocruz.br/bitstream/icict/16556/2/raquel_pinna_etal_IOC_2016.pdf50aaccd9cf457d68842333845f6cd960MD52TEXTraquel_pinna_etal_IOC_2016.pdf.txtraquel_pinna_etal_IOC_2016.pdf.txtExtracted texttext/plain69198https://www.arca.fiocruz.br/bitstream/icict/16556/3/raquel_pinna_etal_IOC_2016.pdf.txt8f36da2ec49f9e5173cdc12f48bd34e2MD53icict/165562018-08-15 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dc.title.pt_BR.fl_str_mv Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
title Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
spellingShingle Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
Pinna, Raquel A.
Malária
Co-infecção
Leishmaniose
Imunologia
Citocina
Co-infection
Malaria
Leishmaniasis
immunology
cytokine
Plasmodium yoelii
Leishmania brasiliensis
Leishmania amazonensis
title_short Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
title_full Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
title_fullStr Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
title_full_unstemmed Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
title_sort Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response
author Pinna, Raquel A.
author_facet Pinna, Raquel A.
Santos, Danielle Silva dos
Silva, Daiana S. Perce da
Ferreira, Joseli Oliveira
Verde, Dea M. S. Vila
Luca, Paula M. de
Banic, Dalma M.
author_role author
author2 Santos, Danielle Silva dos
Silva, Daiana S. Perce da
Ferreira, Joseli Oliveira
Verde, Dea M. S. Vila
Luca, Paula M. de
Banic, Dalma M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinna, Raquel A.
Santos, Danielle Silva dos
Silva, Daiana S. Perce da
Ferreira, Joseli Oliveira
Verde, Dea M. S. Vila
Luca, Paula M. de
Banic, Dalma M.
dc.subject.other.pt_BR.fl_str_mv Malária
Co-infecção
Leishmaniose
Imunologia
Citocina
topic Malária
Co-infecção
Leishmaniose
Imunologia
Citocina
Co-infection
Malaria
Leishmaniasis
immunology
cytokine
Plasmodium yoelii
Leishmania brasiliensis
Leishmania amazonensis
dc.subject.en.pt_BR.fl_str_mv Co-infection
Malaria
Leishmaniasis
immunology
cytokine
Plasmodium yoelii
Leishmania brasiliensis
Leishmania amazonensis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Simulídeos, Oncocercose e Infecções Simpáticas: Mansonerlose e Malária. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-20T10:57:31Z
dc.date.available.fl_str_mv 2016-12-20T10:57:31Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINNA, Raquel A. et al. Malaria - Cutaneous Leishmaniasis Co-infection: influence on disease outcomes and immune response. Frontiers in Microbiology, v.7, Article 982, 15p, June 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16556
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2016.00982
identifier_str_mv PINNA, Raquel A. et al. Malaria - Cutaneous Leishmaniasis Co-infection: influence on disease outcomes and immune response. Frontiers in Microbiology, v.7, Article 982, 15p, June 2016.
1664-302X
10.3389/fmicb.2016.00982
url https://www.arca.fiocruz.br/handle/icict/16556
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontier Media
publisher.none.fl_str_mv Frontier Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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