The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23272 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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Aliança, Amanda Silva dos SantosAnjos, Keicyanne Fernanda Lessa dosReis, Thiago Nogueira de VasconcelosHigino, Taciana Mirely MacielBrelaz-de-Castro, Maria Carolina AcciolyBianco, Éverson MiguelFigueiredo, Regina Celia Bressan Queiroz de2017-11-20T14:30:32Z2017-11-20T14:30:32Z2014ALIANÇA, Amanda Silva dos S. et al. The in Vitro Biological Activity of the Brazilian Brown Seaweed Dictyota mertensii against Leishmania amazonensis. Molecules, v. 19, n. 9, p. 14052–14065, 9 set. 2014.1420-3049https://www.arca.fiocruz.br/handle/icict/2327210.3390/molecules190914052engDictyota mertensiiquimioterapiaLeishmania amazonensiultraestruturaDictyota mertensiichemotherapyLeishmania amazonensiultrastructureAntiprotozoáriosfarmacologiaLeishmaniaefeitos de drogasLeishmaniosetratamento farmacológicoFeófitasquímicaCélulas CultivadasanimaisAntiprotozoáriosisolamento & purificaçãoAvaliação Pré-Clínica de MedicamentosConcentração Inibidora 50Macrófagos PeritoneaismetabolismoThe in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Seaweeds present a wide variety of interesting bioactive molecules. In the present work we evaluated the biological activity of the dichloromethane/methanol (2:1) extract (DME) from the brown seaweed Dictyota mertensii against Leishmania amazonensis and its cytotoxic potential on mammalian cells. The extract showed significant inhibitory effect on the growth of promastigote forms (IC50=71.60 μg/mL) and low toxicity against mammalian cells (CC50=233.10 μg/mL). The DME was also efficient in inhibiting the infection in macrophages, with CC50 of 81.4 μg/mL and significantly decreased the survival of amastigote forms within these cells. The selectivity index showed that DME was more toxic to both promastigote (SI=3.25) and amastigote (SI=2.86) forms than to macrophages. Increased NO production was observed in treated macrophages suggesting that besides acting directly on the parasites, the DME also shows an immunomodulatory effect on macrophages. Drastic ultrastructural alterations consistent with loss of viability and cell death were observed in treated parasites. Confocal microscopy and cytometry analyzes showed no significant impairment of plasma membrane integrity, whereas an intense depolarization of mitochondrial membrane could be observed by using propidium iodide and rhodamine 123 staining, respectively. The low toxicity to mammalian cells and the effective activity against promastigotes and amastigotes, point to the use of DME as a promising agent for the treatment of cutaneous leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23272/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. The in Vitro Biological Activity - aliança.pdfart. 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| dc.title.pt_BR.fl_str_mv |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| title |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| spellingShingle |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis Aliança, Amanda Silva dos Santos Dictyota mertensii quimioterapia Leishmania amazonensi ultraestrutura Dictyota mertensii chemotherapy Leishmania amazonensi ultrastructure Antiprotozoários farmacologia Leishmania efeitos de drogas Leishmaniose tratamento farmacológico Feófitas química Células Cultivadas animais Antiprotozoários isolamento & purificação Avaliação Pré-Clínica de Medicamentos Concentração Inibidora 50 Macrófagos Peritoneais metabolismo |
| title_short |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| title_full |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| title_fullStr |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| title_full_unstemmed |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| title_sort |
The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis |
| author |
Aliança, Amanda Silva dos Santos |
| author_facet |
Aliança, Amanda Silva dos Santos Anjos, Keicyanne Fernanda Lessa dos Reis, Thiago Nogueira de Vasconcelos Higino, Taciana Mirely Maciel Brelaz-de-Castro, Maria Carolina Accioly Bianco, Éverson Miguel Figueiredo, Regina Celia Bressan Queiroz de |
| author_role |
author |
| author2 |
Anjos, Keicyanne Fernanda Lessa dos Reis, Thiago Nogueira de Vasconcelos Higino, Taciana Mirely Maciel Brelaz-de-Castro, Maria Carolina Accioly Bianco, Éverson Miguel Figueiredo, Regina Celia Bressan Queiroz de |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Aliança, Amanda Silva dos Santos Anjos, Keicyanne Fernanda Lessa dos Reis, Thiago Nogueira de Vasconcelos Higino, Taciana Mirely Maciel Brelaz-de-Castro, Maria Carolina Accioly Bianco, Éverson Miguel Figueiredo, Regina Celia Bressan Queiroz de |
| dc.subject.other.pt_BR.fl_str_mv |
Dictyota mertensii quimioterapia Leishmania amazonensi ultraestrutura |
| topic |
Dictyota mertensii quimioterapia Leishmania amazonensi ultraestrutura Dictyota mertensii chemotherapy Leishmania amazonensi ultrastructure Antiprotozoários farmacologia Leishmania efeitos de drogas Leishmaniose tratamento farmacológico Feófitas química Células Cultivadas animais Antiprotozoários isolamento & purificação Avaliação Pré-Clínica de Medicamentos Concentração Inibidora 50 Macrófagos Peritoneais metabolismo |
| dc.subject.en.pt_BR.fl_str_mv |
Dictyota mertensii chemotherapy Leishmania amazonensi ultrastructure |
| dc.subject.decs.pt_BR.fl_str_mv |
Antiprotozoários farmacologia Leishmania efeitos de drogas Leishmaniose tratamento farmacológico Feófitas química Células Cultivadas animais Antiprotozoários isolamento & purificação Avaliação Pré-Clínica de Medicamentos Concentração Inibidora 50 Macrófagos Peritoneais metabolismo |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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2014 |
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2014 |
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2017-11-20T14:30:32Z |
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2017-11-20T14:30:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
ALIANÇA, Amanda Silva dos S. et al. The in Vitro Biological Activity of the Brazilian Brown Seaweed Dictyota mertensii against Leishmania amazonensis. Molecules, v. 19, n. 9, p. 14052–14065, 9 set. 2014. |
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https://www.arca.fiocruz.br/handle/icict/23272 |
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1420-3049 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/molecules190914052 |
| identifier_str_mv |
ALIANÇA, Amanda Silva dos S. et al. The in Vitro Biological Activity of the Brazilian Brown Seaweed Dictyota mertensii against Leishmania amazonensis. Molecules, v. 19, n. 9, p. 14052–14065, 9 set. 2014. 1420-3049 10.3390/molecules190914052 |
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