Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex

Detalhes bibliográficos
Autor(a) principal: Sousa, Leijiane F.
Data de Publicação: 2013
Outros Autores: Nicolau, Carolina A., Peixoto, Pedro S., Bernardoni, Juliana L., Oliveira, Sâmella S., Portes Junior, José Antonio, Mourão, Rosa Helena V., Santos, Isa Lima dos, Martins, Ida S. Sano, Chalkidis, Hipócrates M., Valente, Richard H., Silva, Ana M. Moura da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11780
Resumo: Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.
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spelling Sousa, Leijiane F.Nicolau, Carolina A.Peixoto, Pedro S.Bernardoni, Juliana L.Oliveira, Sâmella S.Portes Junior, José AntonioMourão, Rosa Helena V.Santos, Isa Lima dosMartins, Ida S. SanoChalkidis, Hipócrates M.Valente, Richard H.Silva, Ana M. Moura da2015-09-21T17:25:46Z2015-09-21T17:25:46Z2013SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013.https://www.arca.fiocruz.br/handle/icict/1178010.1371/journal.pntd.0002442engPlos OneSAB – soro antibotrópicoBothropsPhylogenyVenom compositionAntivenomBothropsFilogeniaComparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complexinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo CruzI. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.Universidade São Paulo. Instituto de Matemática e Estatística. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Instituto Butantan. Laboratório de Fisiopatologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Universidade Federal do Oeste do Pará. Santarém, Pará, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Instituto Butantan. Laboratório de Fisiopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Faculdades Integradas do Tapajó. Santarém, Pará, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo CruzI. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.nstituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB – soro antibotro´pico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which venoms should be used as immunizing antigens for the production of pan-specific Bothrops antivenoms. In this study, we simultaneously compared the composition and reactivity with SAB of venoms collected from six species of snakes, distributed in pairs from three distinct phylogenetic clades: Bothrops, Bothropoides and Rhinocerophis. We also evaluated the neutralization of Bothrops atrox venom, which is the species responsible for most snake bites in the Amazon region, but not included in the immunization antigen mixture used to produce SAB. Using mass spectrometric and chromatographic approaches, we observed a lack of similarity in protein composition between the venoms from closely related snakes and a high similarity between the venoms of phylogenetically more distant snakes, suggesting little connection between taxonomic position and venom composition. P-III snake venom metalloproteinases (SVMPs) are the most antigenic toxins in the venoms of snakes from the Bothrops complex, whereas class P-I SVMPs, snake venom serine proteinases and phospholipases A2 reacted with antibodies in lower levels. Low molecular size toxins, such as disintegrins and bradykinin-potentiating peptides, were poorly antigenic. Toxins from the same protein family showed antigenic cross-reactivity among venoms from different species; SAB was efficient in neutralizing the B. atrox venom major toxins. Thus, we suggest that it is possible to obtain pan-specific effective antivenoms for Bothrops envenomations through immunization with venoms from only a few species of snakes, if these venoms contain protein classes that are representative of all species to which the antivenom is targeted.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11780/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcarolina_nicolau_etal_IOC_2013.pdfapplication/pdf1668584https://www.arca.fiocruz.br/bitstream/icict/11780/2/carolina_nicolau_etal_IOC_2013.pdf6ae4f3b359524876188b347a8f43cd15MD52TEXTcarolina_nicolau_etal_IOC_2013.pdf.txtcarolina_nicolau_etal_IOC_2013.pdf.txtExtracted texttext/plain83056https://www.arca.fiocruz.br/bitstream/icict/11780/3/carolina_nicolau_etal_IOC_2013.pdf.txt0f8b734fa456a95e1cfbae01773528d6MD53icict/117802022-06-24 13:11:01.038oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:11:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
title Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
spellingShingle Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
Sousa, Leijiane F.
SAB – soro antibotrópico
Bothrops
Phylogeny
Venom composition
Antivenom
Bothrops
Filogenia
title_short Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
title_full Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
title_fullStr Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
title_full_unstemmed Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
title_sort Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
author Sousa, Leijiane F.
author_facet Sousa, Leijiane F.
Nicolau, Carolina A.
Peixoto, Pedro S.
Bernardoni, Juliana L.
Oliveira, Sâmella S.
Portes Junior, José Antonio
Mourão, Rosa Helena V.
Santos, Isa Lima dos
Martins, Ida S. Sano
Chalkidis, Hipócrates M.
Valente, Richard H.
Silva, Ana M. Moura da
author_role author
author2 Nicolau, Carolina A.
Peixoto, Pedro S.
Bernardoni, Juliana L.
Oliveira, Sâmella S.
Portes Junior, José Antonio
Mourão, Rosa Helena V.
Santos, Isa Lima dos
Martins, Ida S. Sano
Chalkidis, Hipócrates M.
Valente, Richard H.
Silva, Ana M. Moura da
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Leijiane F.
Nicolau, Carolina A.
Peixoto, Pedro S.
Bernardoni, Juliana L.
Oliveira, Sâmella S.
Portes Junior, José Antonio
Mourão, Rosa Helena V.
Santos, Isa Lima dos
Martins, Ida S. Sano
Chalkidis, Hipócrates M.
Valente, Richard H.
Silva, Ana M. Moura da
dc.subject.other.pt_BR.fl_str_mv SAB – soro antibotrópico
topic SAB – soro antibotrópico
Bothrops
Phylogeny
Venom composition
Antivenom
Bothrops
Filogenia
dc.subject.en.pt_BR.fl_str_mv Bothrops
Phylogeny
Venom composition
Antivenom
dc.subject.decs.pt_BR.fl_str_mv Bothrops
Filogenia
description Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:46Z
dc.date.available.fl_str_mv 2015-09-21T17:25:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11780
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0002442
identifier_str_mv SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013.
10.1371/journal.pntd.0002442
url https://www.arca.fiocruz.br/handle/icict/11780
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/11780/2/carolina_nicolau_etal_IOC_2013.pdf
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