Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11780 |
Resumo: | Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil. |
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Sousa, Leijiane F.Nicolau, Carolina A.Peixoto, Pedro S.Bernardoni, Juliana L.Oliveira, Sâmella S.Portes Junior, José AntonioMourão, Rosa Helena V.Santos, Isa Lima dosMartins, Ida S. SanoChalkidis, Hipócrates M.Valente, Richard H.Silva, Ana M. Moura da2015-09-21T17:25:46Z2015-09-21T17:25:46Z2013SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013.https://www.arca.fiocruz.br/handle/icict/1178010.1371/journal.pntd.0002442engPlos OneSAB – soro antibotrópicoBothropsPhylogenyVenom compositionAntivenomBothropsFilogeniaComparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complexinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo CruzI. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.Universidade São Paulo. Instituto de Matemática e Estatística. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Instituto Butantan. Laboratório de Fisiopatologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Universidade Federal do Oeste do Pará. Santarém, Pará, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Instituto Butantan. Laboratório de Fisiopatologia. São Paulo, SP, Brasil.Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil / Faculdades Integradas do Tapajó. Santarém, Pará, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo CruzI. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.nstituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas (INCTTox/CNPq). Brasil.In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB – soro antibotro´pico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which venoms should be used as immunizing antigens for the production of pan-specific Bothrops antivenoms. In this study, we simultaneously compared the composition and reactivity with SAB of venoms collected from six species of snakes, distributed in pairs from three distinct phylogenetic clades: Bothrops, Bothropoides and Rhinocerophis. We also evaluated the neutralization of Bothrops atrox venom, which is the species responsible for most snake bites in the Amazon region, but not included in the immunization antigen mixture used to produce SAB. Using mass spectrometric and chromatographic approaches, we observed a lack of similarity in protein composition between the venoms from closely related snakes and a high similarity between the venoms of phylogenetically more distant snakes, suggesting little connection between taxonomic position and venom composition. P-III snake venom metalloproteinases (SVMPs) are the most antigenic toxins in the venoms of snakes from the Bothrops complex, whereas class P-I SVMPs, snake venom serine proteinases and phospholipases A2 reacted with antibodies in lower levels. Low molecular size toxins, such as disintegrins and bradykinin-potentiating peptides, were poorly antigenic. Toxins from the same protein family showed antigenic cross-reactivity among venoms from different species; SAB was efficient in neutralizing the B. atrox venom major toxins. Thus, we suggest that it is possible to obtain pan-specific effective antivenoms for Bothrops envenomations through immunization with venoms from only a few species of snakes, if these venoms contain protein classes that are representative of all species to which the antivenom is targeted.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11780/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcarolina_nicolau_etal_IOC_2013.pdfapplication/pdf1668584https://www.arca.fiocruz.br/bitstream/icict/11780/2/carolina_nicolau_etal_IOC_2013.pdf6ae4f3b359524876188b347a8f43cd15MD52TEXTcarolina_nicolau_etal_IOC_2013.pdf.txtcarolina_nicolau_etal_IOC_2013.pdf.txtExtracted texttext/plain83056https://www.arca.fiocruz.br/bitstream/icict/11780/3/carolina_nicolau_etal_IOC_2013.pdf.txt0f8b734fa456a95e1cfbae01773528d6MD53icict/117802022-06-24 13:11:01.038oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:11:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
title |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
spellingShingle |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex Sousa, Leijiane F. SAB – soro antibotrópico Bothrops Phylogeny Venom composition Antivenom Bothrops Filogenia |
title_short |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
title_full |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
title_fullStr |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
title_full_unstemmed |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
title_sort |
Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex |
author |
Sousa, Leijiane F. |
author_facet |
Sousa, Leijiane F. Nicolau, Carolina A. Peixoto, Pedro S. Bernardoni, Juliana L. Oliveira, Sâmella S. Portes Junior, José Antonio Mourão, Rosa Helena V. Santos, Isa Lima dos Martins, Ida S. Sano Chalkidis, Hipócrates M. Valente, Richard H. Silva, Ana M. Moura da |
author_role |
author |
author2 |
Nicolau, Carolina A. Peixoto, Pedro S. Bernardoni, Juliana L. Oliveira, Sâmella S. Portes Junior, José Antonio Mourão, Rosa Helena V. Santos, Isa Lima dos Martins, Ida S. Sano Chalkidis, Hipócrates M. Valente, Richard H. Silva, Ana M. Moura da |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Leijiane F. Nicolau, Carolina A. Peixoto, Pedro S. Bernardoni, Juliana L. Oliveira, Sâmella S. Portes Junior, José Antonio Mourão, Rosa Helena V. Santos, Isa Lima dos Martins, Ida S. Sano Chalkidis, Hipócrates M. Valente, Richard H. Silva, Ana M. Moura da |
dc.subject.other.pt_BR.fl_str_mv |
SAB – soro antibotrópico |
topic |
SAB – soro antibotrópico Bothrops Phylogeny Venom composition Antivenom Bothrops Filogenia |
dc.subject.en.pt_BR.fl_str_mv |
Bothrops Phylogeny Venom composition Antivenom |
dc.subject.decs.pt_BR.fl_str_mv |
Bothrops Filogenia |
description |
Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2015-09-21T17:25:46Z |
dc.date.available.fl_str_mv |
2015-09-21T17:25:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/11780 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pntd.0002442 |
identifier_str_mv |
SOUSA, Leijiane F.; et al. Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex. PLoS Negl Trop Dis., v.7, n.9, e2442, 16p, Sept. 2013. 10.1371/journal.pntd.0002442 |
url |
https://www.arca.fiocruz.br/handle/icict/11780 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Plos One |
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Plos One |
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Repositório Institucional da FIOCRUZ (ARCA) |
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