Potential Therapeutic Agents for Feline Calicivirus Infection

Detalhes bibliográficos
Autor(a) principal: Fumian, Tulio M.
Data de Publicação: 2018
Outros Autores: Tuipulotu, Daniel Enosi, Netzler, Natalie E., Lun, Jennifer H., Russo, Alice G., Yan, Grace J. H., White, Peter A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31635
Resumo: University of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, Australia / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
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spelling Fumian, Tulio M.Tuipulotu, Daniel EnosiNetzler, Natalie E.Lun, Jennifer H.Russo, Alice G.Yan, Grace J. H.White, Peter A.2019-02-14T12:21:27Z2019-02-14T12:21:27Z2018FUMIAN, Tulio Machado; et al. Potential Therapeutic Agents for Feline Calicivirus Infection. Viruses, v.10, n.433, 16p, 2018.1999-4915https://www.arca.fiocruz.br/handle/icict/3163510.3390/v100804331999-4915engMDPIAntiviraisCalicivírus felinoInibidores não nucleósidosAnálogos nucleosídeosInibidores de proteaseFeline calicivirusAntiviralsNucleoside analoguesNon-nucleoside inhibitorsProtease inhibitorsPotential Therapeutic Agents for Feline Calicivirus Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, Australia / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.University of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.University of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, AustraliaUniversity of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, AustraliaUniversity of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, AustraliaUniversity of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, AustraliaUniversity of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, AustraliaFeline calicivirus (FCV) is a major cause of upper respiratory tract disease in cats, with widespread distribution in the feline population. Recently, virulent systemic diseases caused by FCV infection has been associated with mortality rates up to 50%. Currently, there are no direct-acting antivirals approved for the treatment of FCV infection. Here, we tested 15 compounds from different antiviral classes against FCV using in vitro protein and cell culture assays. After the expression of FCV protease-polymerase protein, we established two in vitro assays to assess the inhibitory activity of compounds directly against the FCV protease or polymerase. Using this recombinant enzyme, we identified quercetagetin and PPNDS as inhibitors of FCV polymerase activity (IC50 values of 2.8 μM and 2.7 μM, respectively). We also demonstrate the inhibition of FCV protease activity by GC376 (IC50 of 18 µM). Using cell culture assays, PPNDS, quercetagetin and GC376 did not display antivirals effects, however, we identified nitazoxanide and 2'-C-methylcytidine (2CMC) as potent inhibitors of FCV replication, with EC50 values in the low micromolar range (0.6 μM and 2.5 μM, respectively). In conclusion, we established two in vitro assays that will accelerate the research for FCV antivirals and can be used for the high-throughput screening of direct-acting antivirals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31635/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALtulio2_fumian_etal_IOC_2018.pdftulio2_fumian_etal_IOC_2018.pdfapplication/pdf8541693https://www.arca.fiocruz.br/bitstream/icict/31635/2/tulio2_fumian_etal_IOC_2018.pdf886a727e965d64d86d7679b29d9a5aabMD52TEXTtulio2_fumian_etal_IOC_2018.pdf.txttulio2_fumian_etal_IOC_2018.pdf.txtExtracted texttext/plain60072https://www.arca.fiocruz.br/bitstream/icict/31635/3/tulio2_fumian_etal_IOC_2018.pdf.txta2e55c178799fc4d5ee47a269d8ea151MD53icict/316352019-02-15 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dc.title.pt_BR.fl_str_mv Potential Therapeutic Agents for Feline Calicivirus Infection
title Potential Therapeutic Agents for Feline Calicivirus Infection
spellingShingle Potential Therapeutic Agents for Feline Calicivirus Infection
Fumian, Tulio M.
Antivirais
Calicivírus felino
Inibidores não nucleósidos
Análogos nucleosídeos
Inibidores de protease
Feline calicivirus
Antivirals
Nucleoside analogues
Non-nucleoside inhibitors
Protease inhibitors
title_short Potential Therapeutic Agents for Feline Calicivirus Infection
title_full Potential Therapeutic Agents for Feline Calicivirus Infection
title_fullStr Potential Therapeutic Agents for Feline Calicivirus Infection
title_full_unstemmed Potential Therapeutic Agents for Feline Calicivirus Infection
title_sort Potential Therapeutic Agents for Feline Calicivirus Infection
author Fumian, Tulio M.
author_facet Fumian, Tulio M.
Tuipulotu, Daniel Enosi
Netzler, Natalie E.
Lun, Jennifer H.
Russo, Alice G.
Yan, Grace J. H.
White, Peter A.
author_role author
author2 Tuipulotu, Daniel Enosi
Netzler, Natalie E.
Lun, Jennifer H.
Russo, Alice G.
Yan, Grace J. H.
White, Peter A.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fumian, Tulio M.
Tuipulotu, Daniel Enosi
Netzler, Natalie E.
Lun, Jennifer H.
Russo, Alice G.
Yan, Grace J. H.
White, Peter A.
dc.subject.other.pt_BR.fl_str_mv Antivirais
Calicivírus felino
Inibidores não nucleósidos
Análogos nucleosídeos
Inibidores de protease
topic Antivirais
Calicivírus felino
Inibidores não nucleósidos
Análogos nucleosídeos
Inibidores de protease
Feline calicivirus
Antivirals
Nucleoside analogues
Non-nucleoside inhibitors
Protease inhibitors
dc.subject.en.pt_BR.fl_str_mv Feline calicivirus
Antivirals
Nucleoside analogues
Non-nucleoside inhibitors
Protease inhibitors
description University of New South Wales. Faculty of Science. School of Biotechnology and Biomolecular Sciences. Sydney, Australia / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-14T12:21:27Z
dc.date.available.fl_str_mv 2019-02-14T12:21:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FUMIAN, Tulio Machado; et al. Potential Therapeutic Agents for Feline Calicivirus Infection. Viruses, v.10, n.433, 16p, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31635
dc.identifier.issn.pt_BR.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v10080433
dc.identifier.eissn.none.fl_str_mv 1999-4915
identifier_str_mv FUMIAN, Tulio Machado; et al. Potential Therapeutic Agents for Feline Calicivirus Infection. Viruses, v.10, n.433, 16p, 2018.
1999-4915
10.3390/v10080433
url https://www.arca.fiocruz.br/handle/icict/31635
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31635/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31635/2/tulio2_fumian_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/31635/3/tulio2_fumian_etal_IOC_2018.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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