In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract

Detalhes bibliográficos
Autor(a) principal: Mota, Fabio Faria
Data de Publicação: 2019
Outros Autores: Castro, Daniele Pereira, Vieira, Cecilia Stahl, Gumiel, Marcia, Albuquerque, Julia Peixoto de, Carels, Nicolas, Azambuja, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fmicb.2018.03205
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32851
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Mota, Fabio FariaCastro, Daniele PereiraVieira, Cecilia StahlGumiel, MarciaAlbuquerque, Julia Peixoto deCarels, NicolasAzambuja, Patricia2019-04-29T17:41:25Z2019-04-29T17:41:25Z2019MOTA, Fabio Faria da et al. In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract. Frontiers in Microbiology, v. 9, p. 1-17, Jan. 2019.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3285110.3389/fmicb.2018.03205engFrontiers MediaSerratia marcescensRhodnius prolixusTrypanosoma cruziGenes AntagonistasAtividade tripanocidaSerratia marcescensRhodnius prolixusTrypanosoma cruziAntagonistic genesTrypanocidal activityIn vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tractinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia em Inovação em Doenças Negligenciadas. Laboratório de Modelagem de Sistemas Biológicos. Rio de Janeiro, RJ. Brasil.Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Serratia marcescens is a bacterium with the ability to colonize several niches, including some eukaryotic hosts. S. marcescens have been recently found in the gut of hematophagous insects that act as parasite vectors, such as Anopheles, Rhodnius, and Triatoma. While some S. marcescens strains have been reported as symbiotic or pathogenic to other insects, the role of S. marcescens populations from the gut microbiota of Rhodnius prolixus, a vector of Chagas' disease, remains unknown. Bacterial colonies from R. prolixus gut were isolated on BHI agar. After BOX-PCR fingerprinting, the genomic sequences of two isolates RPA1 and RPH1 were compared to others S. marcescens from the NCBI database in other to estimate their evolutionary divergence. The in vitro trypanolytic activity of these two bacterial isolates against Trypanosoma cruzi (DM28c clone and Y strain) was assessed by microscopy. In addition, the gene expression of type VI secretion system (T6SS) was detected in vivo by RT-PCR. Comparative genomics of RPA1 and RPH1 revealed, besides plasmid presence and genomic islands, genes related to motility, attachment, and quorum sensing in both genomes while genes for urea hydrolysis and type II secretion system (T2SS) were found only in the RPA1 genome. The in vitro trypanolytic activity of both S. marcescens strains was stronger in their stationary phases of growth than in their exponential ones, with 65-70 and 85-90% of epimastigotes (Dm28c clone and Y strain, respectively) being lysed after incubation with RPA1 or RPH1 in stationary phase. Although T6SS transcripts were detected in guts up to 40 days after feeding (DAF), R. prolixus morbidity or mortality did not appear to be affected. In this report, we made available two trypanolytic S. marcescens strains from R. prolixus gut to the scientific community together with their genomic sequences. Here, we describe their genomic features with the purpose of bringing new insights into the S. marcescens adaptations for colonization of the specific niche of triatomine guts. This study provides the basis for a better understanding of the role of S. marcescens in the microbiota of R. prolixus gut as a potential antagonist of T. cruzi in this complex system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32851/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFabioFaria_Mota_etal_IOC_2019.pdfFabioFaria_Mota_etal_IOC_2019.pdfapplication/pdf4237942https://www.arca.fiocruz.br/bitstream/icict/32851/2/FabioFaria_Mota_etal_IOC_2019.pdfd580927bcebfc83c0dcafba666d7274bMD52TEXTFabioFaria_Mota_etal_IOC_2019.pdf.txtFabioFaria_Mota_etal_IOC_2019.pdf.txtExtracted texttext/plain91700https://www.arca.fiocruz.br/bitstream/icict/32851/3/FabioFaria_Mota_etal_IOC_2019.pdf.txt076c25b6725b7ca3166d63e47e4c38ccMD53icict/328512023-07-13 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dc.title.pt_BR.fl_str_mv In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
title In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
spellingShingle In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
Mota, Fabio Faria
Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Genes Antagonistas
Atividade tripanocida
Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Antagonistic genes
Trypanocidal activity
title_short In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
title_full In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
title_fullStr In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
title_full_unstemmed In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
title_sort In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
author Mota, Fabio Faria
author_facet Mota, Fabio Faria
Castro, Daniele Pereira
Vieira, Cecilia Stahl
Gumiel, Marcia
Albuquerque, Julia Peixoto de
Carels, Nicolas
Azambuja, Patricia
author_role author
author2 Castro, Daniele Pereira
Vieira, Cecilia Stahl
Gumiel, Marcia
Albuquerque, Julia Peixoto de
Carels, Nicolas
Azambuja, Patricia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mota, Fabio Faria
Castro, Daniele Pereira
Vieira, Cecilia Stahl
Gumiel, Marcia
Albuquerque, Julia Peixoto de
Carels, Nicolas
Azambuja, Patricia
dc.subject.other.pt_BR.fl_str_mv Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Genes Antagonistas
Atividade tripanocida
topic Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Genes Antagonistas
Atividade tripanocida
Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Antagonistic genes
Trypanocidal activity
dc.subject.en.pt_BR.fl_str_mv Serratia marcescens
Rhodnius prolixus
Trypanosoma cruzi
Antagonistic genes
Trypanocidal activity
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-04-29T17:41:25Z
dc.date.available.fl_str_mv 2019-04-29T17:41:25Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MOTA, Fabio Faria da et al. In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract. Frontiers in Microbiology, v. 9, p. 1-17, Jan. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32851
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.03205
identifier_str_mv MOTA, Fabio Faria da et al. In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract. Frontiers in Microbiology, v. 9, p. 1-17, Jan. 2019.
1664-302X
10.3389/fmicb.2018.03205
url https://www.arca.fiocruz.br/handle/icict/32851
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Frontiers Media
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