Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13242 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
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Cruz, Helena MedinaScalioni, Letícia de PaulaPaula, Vanessa Salete deSilva, Elisângela Ferreira daÓ, Kycia Maria Rodrigues doMilagres, Flavio Augusto PáduaCruz, Marcelo SantosBastos, Francisco Inácio Pinkusfeld MonteiroPollo-Flores, PriscilaLeal, ErotildesCastro, Ana Rita Coimbra MottaPilotto, José HenriqueLewis-Ximenez, Lia LauraLampe, ElisabethVillar, Livia Melo2016-03-22T15:38:21Z2016-03-22T15:38:21Z2015CRUZ, Helena Medina; et al. Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations. BMC Infectious Disease, v.15:548, 10p, 2015.1471-2334https://www.arca.fiocruz.br/handle/icict/1324210.1186/s12879-015-1249-5engBioMed CentralTestes de desempenhoVírus da hepatite BTécnicas e Procedimentos DiagnósticosHepatitis B virusRapid testsPerformance of testsDiagnostic proceduresEvaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.São Lucas Hospital. Petrópolis, Rio de Janeiro, RJ, Brasil.Universidade Federal de Tocantins. Faculdade de Medicina. Palmas, TO, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Psiquiatria. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnológica em Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Hospital Universitário Antonio Pedro. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Campus Macaé. Macaé, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz-MS. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Background: Rapid tests (RTs) might have several advantages over standard laboratory procedures, increasing access to diagnosis, especially among vulnerable populations and/or those living in remote areas. The aim of this study was to evaluate the performance of RTs for the detection of hepatitis B virus surface antigen (HBsAg) in samples from different populations/settings. Methods: Three RTs for HBsAg detection (Vikia® HBsAg, HBsAg Teste Rápido®, and Imuno-Rápido HBsAg®) and different biological specimens (serum, whole blood, and saliva) were evaluated. Analyses comprised a reference panel and samples from field studies targeting suspected cases of hepatitis B virus (HBV) (G I), individuals living in deprived areas (G II), and highly vulnerable individuals (G III). Enzyme immunoassay (EIA) was defined as the gold standard in this study. Reproducibility, repeatability, and cross-reactivity with other infectious agents such as dengue, immunodeficiency (HIV), and hepatitis C (HCV) viruses and T. pallidum were determined. Results: For the reference panel, the sensitivity and specificity of all HBsAg RTs were higher than 93.00 %. G I presented the highest kappa values for all rapid assays using sera samples. When using serum, the sensitivity values were higher than 93.40 for G I, 60.00 % for G II and 66.77 % for G III, and the specificity values were higher than 99.50 for GI, 97.20 for G II and 99.10 % for G III for all tests. For whole blood samples & the Vikia® HBsAg assay, the best performance was achieved for GIII (k = 79.75 %). For saliva samples, the Imuno-Rápido HBsAg® assay showed the highest concordance values with EIA for G I (40.68 %) and G II (32.20 %). The reproducibility and repeatability of all RTs for serum and saliva were excellent, and the concordance between HBsAg EIAs and RTs using samples reactive with other infectious agents varied from 70.10 % to 100.00 %. Conclusions: The overall performance of RTs for HBsAg in serum was high/moderately high for all groups, thereby promoting increased access to HBV diagnosis among vulnerable populations as well as samples from individuals in emergency settings or remote areas. Rapid tests for HBsAg using whole blood could be used in prevalence studies, though these assays should not be used for saliva samples.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13242/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALleticia_saclioni_etal_IOC_2015.pdfleticia_saclioni_etal_IOC_2015.pdfapplication/pdf432581https://www.arca.fiocruz.br/bitstream/icict/13242/2/leticia_saclioni_etal_IOC_2015.pdf77770e34cf6da4970e8e1c774807f64fMD52TEXTleticia_saclioni_etal_IOC_2015.pdf.txtleticia_saclioni_etal_IOC_2015.pdf.txtExtracted texttext/plain43886https://www.arca.fiocruz.br/bitstream/icict/13242/3/leticia_saclioni_etal_IOC_2015.pdf.txtd61108dc1ce152b3b1e9f396e295a2e6MD53icict/132422023-09-05 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| dc.title.pt_BR.fl_str_mv |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| title |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| spellingShingle |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations Cruz, Helena Medina Testes de desempenho Vírus da hepatite B Técnicas e Procedimentos Diagnósticos Hepatitis B virus Rapid tests Performance of tests Diagnostic procedures |
| title_short |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| title_full |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| title_fullStr |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| title_full_unstemmed |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| title_sort |
Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations |
| author |
Cruz, Helena Medina |
| author_facet |
Cruz, Helena Medina Scalioni, Letícia de Paula Paula, Vanessa Salete de Silva, Elisângela Ferreira da Ó, Kycia Maria Rodrigues do Milagres, Flavio Augusto Pádua Cruz, Marcelo Santos Bastos, Francisco Inácio Pinkusfeld Monteiro Pollo-Flores, Priscila Leal, Erotildes Castro, Ana Rita Coimbra Motta Pilotto, José Henrique Lewis-Ximenez, Lia Laura Lampe, Elisabeth Villar, Livia Melo |
| author_role |
author |
| author2 |
Scalioni, Letícia de Paula Paula, Vanessa Salete de Silva, Elisângela Ferreira da Ó, Kycia Maria Rodrigues do Milagres, Flavio Augusto Pádua Cruz, Marcelo Santos Bastos, Francisco Inácio Pinkusfeld Monteiro Pollo-Flores, Priscila Leal, Erotildes Castro, Ana Rita Coimbra Motta Pilotto, José Henrique Lewis-Ximenez, Lia Laura Lampe, Elisabeth Villar, Livia Melo |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cruz, Helena Medina Scalioni, Letícia de Paula Paula, Vanessa Salete de Silva, Elisângela Ferreira da Ó, Kycia Maria Rodrigues do Milagres, Flavio Augusto Pádua Cruz, Marcelo Santos Bastos, Francisco Inácio Pinkusfeld Monteiro Pollo-Flores, Priscila Leal, Erotildes Castro, Ana Rita Coimbra Motta Pilotto, José Henrique Lewis-Ximenez, Lia Laura Lampe, Elisabeth Villar, Livia Melo |
| dc.subject.other.pt_BR.fl_str_mv |
Testes de desempenho Vírus da hepatite B Técnicas e Procedimentos Diagnósticos |
| topic |
Testes de desempenho Vírus da hepatite B Técnicas e Procedimentos Diagnósticos Hepatitis B virus Rapid tests Performance of tests Diagnostic procedures |
| dc.subject.en.pt_BR.fl_str_mv |
Hepatitis B virus Rapid tests Performance of tests Diagnostic procedures |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2016-03-22T15:38:21Z |
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2016-03-22T15:38:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CRUZ, Helena Medina; et al. Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations. BMC Infectious Disease, v.15:548, 10p, 2015. |
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https://www.arca.fiocruz.br/handle/icict/13242 |
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1471-2334 |
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10.1186/s12879-015-1249-5 |
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CRUZ, Helena Medina; et al. Evaluating HBsAg rapid test performance for different biological samples from low and high infection rate settings & populations. BMC Infectious Disease, v.15:548, 10p, 2015. 1471-2334 10.1186/s12879-015-1249-5 |
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BioMed Central |
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BioMed Central |
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