Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.

Detalhes bibliográficos
Autor(a) principal: Fiuza, Jacqueline Araújo
Data de Publicação: 2013
Outros Autores: Santiago, Helton da Costa, Gannavaram, Angamuthu Selvapandiyane Sreenivas, Ricci, Natasha Delaqua, Bueno, Lilian Lacerda, Bartholomeu, Daniella Castanheira, Oliveira, Rodrigo Correa de, Nakhasi, Hira Lal, Fujiwara, Ricardo Toshio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30738
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Division of Emerging and Transfusion Transmitted Diseases. Office of Blood Research and Review. Center for Biologics Evaluation and Research. Food and Drug Administration. Bethesda, MD, USA
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spelling Fiuza, Jacqueline AraújoSantiago, Helton da CostaGannavaram, Angamuthu Selvapandiyane SreenivasRicci, Natasha DelaquaBueno, Lilian LacerdaBartholomeu, Daniella CastanheiraOliveira, Rodrigo Correa deNakhasi, Hira LalFujiwara, Ricardo Toshio2018-12-21T18:18:47Z2018-12-21T18:18:47Z2013FIUZA, Jacqueline Araújo et al. Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs. Vaccine., v. 31, n. 14, p. 1785-1792, 20130264-410Xhttps://www.arca.fiocruz.br/handle/icict/3073810.1016/j.vaccine.2013.01.048engElsevier ScienceLeishmaniose visceralvisceral leishmaniasisInduction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Division of Emerging and Transfusion Transmitted Diseases. Office of Blood Research and Review. Center for Biologics Evaluation and Research. Food and Drug Administration. Bethesda, MD, USAUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilDivision of Emerging and Transfusion Transmitted Diseases. Office of Blood Research and Review. Center for Biologics Evaluation and Research. Food and Drug Administration. Bethesda, MD, USAUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilDivision of Emerging and Transfusion Transmitted Diseases. Office of Blood Research and Review. Center for Biologics Evaluation and Research. Food and Drug Administration. Bethesda, MD, USAFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilZoonotic visceral leishmaniasis, caused by the intracellular protozoan parasite Leishmania infantum, is a neglected tropical disease that is often fatal when untreated. Dogs are considered the main reservoir of L. infantum in zoonotic VL as the presence of infected dogs may increase the risk for human infection. Canine visceral leishmaniasis (CVL) is a major veterinary and public health problem in Southern Europe, Middle East and South America. Control of animal reservoirs relies on elimination of seropositive dogs in endemic areas. However, treatment of infected dogs is not considered a favorable approach as this can lead to emergence of drug resistance since the same drugs are used to treat human infections. Therefore, vaccination against CVL remains the best alternative in control of the animal reservoirs. In this study, we present data on the immunogenicity profile of a live attenuated parasite LdCen(-/-) in a canine infection model and compared it to that of Leishmune(®), a commercially available recombinant vaccine. The immunogenicity of the LdCen(-/-) parasites was evaluated by antibody secretion, production of intracytoplasmic and secreted cytokines, activation and proliferation of T cells. Vaccination with LdCen(-/-) resulted in high immunogenicity as revealed by the higher IgGTotal, IgG1, and IgG2 production and higher lymphoproliferative response. Further, LdCen(-/-) vaccinated dogs showed higher frequencies of activated CD4+ and CD8+ T cells, IFN-γ production by CD8+ T cells, increased secretion of TNF-α and IL-12/IL-23p40 and decreased secretion of IL-4. These results contribute to the understanding of immunogenicity elicited by live attenuated L. donovani parasites and, consequently, to the development of effective vaccines against visceral leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30738/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALInduction of immunogenicity by live attenuated Leishmania .pdfInduction of immunogenicity by live attenuated Leishmania .pdfapplication/pdf4710843https://www.arca.fiocruz.br/bitstream/icict/30738/2/Induction%20of%20immunogenicity%20by%20live%20attenuated%20Leishmania%20.pdf5d93f689eac12056562da506db679037MD52TEXTInduction of immunogenicity by live attenuated Leishmania .pdf.txtInduction of immunogenicity by live attenuated Leishmania .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
title Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
spellingShingle Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
Fiuza, Jacqueline Araújo
Leishmaniose visceral
visceral leishmaniasis
title_short Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
title_full Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
title_fullStr Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
title_full_unstemmed Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
title_sort Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs.
author Fiuza, Jacqueline Araújo
author_facet Fiuza, Jacqueline Araújo
Santiago, Helton da Costa
Gannavaram, Angamuthu Selvapandiyane Sreenivas
Ricci, Natasha Delaqua
Bueno, Lilian Lacerda
Bartholomeu, Daniella Castanheira
Oliveira, Rodrigo Correa de
Nakhasi, Hira Lal
Fujiwara, Ricardo Toshio
author_role author
author2 Santiago, Helton da Costa
Gannavaram, Angamuthu Selvapandiyane Sreenivas
Ricci, Natasha Delaqua
Bueno, Lilian Lacerda
Bartholomeu, Daniella Castanheira
Oliveira, Rodrigo Correa de
Nakhasi, Hira Lal
Fujiwara, Ricardo Toshio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fiuza, Jacqueline Araújo
Santiago, Helton da Costa
Gannavaram, Angamuthu Selvapandiyane Sreenivas
Ricci, Natasha Delaqua
Bueno, Lilian Lacerda
Bartholomeu, Daniella Castanheira
Oliveira, Rodrigo Correa de
Nakhasi, Hira Lal
Fujiwara, Ricardo Toshio
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
topic Leishmaniose visceral
visceral leishmaniasis
dc.subject.en.pt_BR.fl_str_mv visceral leishmaniasis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Division of Emerging and Transfusion Transmitted Diseases. Office of Blood Research and Review. Center for Biologics Evaluation and Research. Food and Drug Administration. Bethesda, MD, USA
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-12-21T18:18:47Z
dc.date.available.fl_str_mv 2018-12-21T18:18:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIUZA, Jacqueline Araújo et al. Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs. Vaccine., v. 31, n. 14, p. 1785-1792, 2013
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30738
dc.identifier.issn.pt_BR.fl_str_mv 0264-410X
dc.identifier.doi.none.fl_str_mv 10.1016/j.vaccine.2013.01.048
identifier_str_mv FIUZA, Jacqueline Araújo et al. Induction of immunogenicity by live attenuated Leishmania donovani centrin deleted parasites in dogs. Vaccine., v. 31, n. 14, p. 1785-1792, 2013
0264-410X
10.1016/j.vaccine.2013.01.048
url https://www.arca.fiocruz.br/handle/icict/30738
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30738/1/license.txt
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