Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection

Detalhes bibliográficos
Autor(a) principal: Martins Júnior, Ronaldo Bragança
Data de Publicação: 2014
Outros Autores: Carney, Sharon, Goldemberg, Daniel, Bonine, Lucas, Spano, Liliana Cruz, Siqueira, Marilda Agudo Mendonça Teixeira de, Checon, Rita Elizabeth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10828
Resumo: Universidade Federal do Espírito Santo.Núcleo de Doenças Infecciosas. Laboratório de Vírus Respiratórios. Vitória, ES. Brasil.
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spelling Martins Júnior, Ronaldo BragançaCarney, SharonGoldemberg, DanielBonine, LucasSpano, Liliana CruzSiqueira, Marilda Agudo Mendonça Teixeira deChecon, Rita Elizabeth2015-06-12T13:57:53Z2015-06-12T13:57:53Z2014MARTINS JÚNIOR, Ronaldo Bragança et al. Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection. Mem Inst Oswaldo Cruz, v.109, n.6, p.716-721, Sept. 2014.1678-8060https://www.arca.fiocruz.br/handle/icict/1082810.1590/0074-0276140046engFundação Oswaldo CruzDetection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo.Núcleo de Doenças Infecciosas. Laboratório de Vírus Respiratórios. Vitória, ES. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública do Espírito Santo. Vitória, ES, Brasil.Universidade Federal do Espírito Santo.Núcleo de Doenças Infecciosas. Laboratório de Vírus Respiratórios. Vitória, ES. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios. Rio de Janeiro, RJ, Brasil.Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirates were obtained from 162 individuals between August 2007-August 2009. Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). Through IIF, 33 (20.4%) specimens with respiratory virus were recognised, with influenza virus representing over half of the positive samples. Through a multiplex real-time RT-PCR assay, 88 (54.3%) positive samples were detected; the most prevalent respiratory viral pathogens were influenza, human rhinovirus and respiratory syncytial virus (RSV). Six cases of viral co-detection were observed, mainly involving RSV. The use of multiplex real-time RT-PCR increased the viral detection by 33.9% and revealed a larger number of respiratory viruses implicated in ARI cases, including the most recently described respiratory viruses [human bocavirus, human metapneumovirus, influenza A (H1N1) pdm09 virus, human coronavirus (HCoV) NL63 and HCoV HKU1].Respiratory infectionsReal-time PCRMultiplexVirusesReação em Cadeia da PolimeraseVirusinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10828/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALsharon_carneyetal_IOC-2014.pdfapplication/pdf337612https://www.arca.fiocruz.br/bitstream/icict/10828/2/sharon_carneyetal_IOC-2014.pdf4eed4ed197556f73cbedf15341cf0889MD52TEXTsharon_carneyetal_IOC-2014.pdf.txtsharon_carneyetal_IOC-2014.pdf.txtExtracted texttext/plain29006https://www.arca.fiocruz.br/bitstream/icict/10828/3/sharon_carneyetal_IOC-2014.pdf.txtc8077fcb7711ab3c2c4215ae771afd12MD53icict/108282022-06-24 12:19:40.455oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
title Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
spellingShingle Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
Martins Júnior, Ronaldo Bragança
Respiratory infections
Real-time PCR
Multiplex
Viruses
Reação em Cadeia da Polimerase
Virus
title_short Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
title_full Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
title_fullStr Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
title_full_unstemmed Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
title_sort Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
author Martins Júnior, Ronaldo Bragança
author_facet Martins Júnior, Ronaldo Bragança
Carney, Sharon
Goldemberg, Daniel
Bonine, Lucas
Spano, Liliana Cruz
Siqueira, Marilda Agudo Mendonça Teixeira de
Checon, Rita Elizabeth
author_role author
author2 Carney, Sharon
Goldemberg, Daniel
Bonine, Lucas
Spano, Liliana Cruz
Siqueira, Marilda Agudo Mendonça Teixeira de
Checon, Rita Elizabeth
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins Júnior, Ronaldo Bragança
Carney, Sharon
Goldemberg, Daniel
Bonine, Lucas
Spano, Liliana Cruz
Siqueira, Marilda Agudo Mendonça Teixeira de
Checon, Rita Elizabeth
dc.subject.en.pt_BR.fl_str_mv Respiratory infections
Real-time PCR
Multiplex
Viruses
topic Respiratory infections
Real-time PCR
Multiplex
Viruses
Reação em Cadeia da Polimerase
Virus
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase
Virus
description Universidade Federal do Espírito Santo.Núcleo de Doenças Infecciosas. Laboratório de Vírus Respiratórios. Vitória, ES. Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-12T13:57:53Z
dc.date.available.fl_str_mv 2015-06-12T13:57:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS JÚNIOR, Ronaldo Bragança et al. Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection. Mem Inst Oswaldo Cruz, v.109, n.6, p.716-721, Sept. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10828
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-0276140046
identifier_str_mv MARTINS JÚNIOR, Ronaldo Bragança et al. Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection. Mem Inst Oswaldo Cruz, v.109, n.6, p.716-721, Sept. 2014.
1678-8060
10.1590/0074-0276140046
url https://www.arca.fiocruz.br/handle/icict/10828
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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